Characterization on Modification and Biocompatibility of PCL Scaffold Prepared with Near-field Direct-writing Melt Electrospinning

Abstract

In this study, orthogonal experiments were designed to explore the optimal process parameters for preparing polycaprolactone(PCL) scaffolds by the near-field direct-writing melt electrospinning(NFDWMES) technology. Based on the optimal process parameters, the PCL scaffolds with different thicknesses, gaps and structures were manufactured and the corresponding hydrophilicities were characterized. The PCL scaffolds were modified by chitosan (CS) and hyaluronic acid(HA) to improve biocompatibility and hydrophilicity. Both Fourier transform infrared spectroscopy(FTIR) analysis and antibacterial experimental results show that the chitosan and hyaluronic acid adhere to the surface of PCL scaffolds, suggesting that the modification plays a positive role in biocompatibility and antibacterial effect. The PCL scaffolds were then employed as a carrier to culture cells. The morphology and distribution of the cells observed by a fluorescence microscope demonstrate that the modified PCL scaffolds have good biocompatibility, and the porous structure of the scaffolds is conducive to adhesion and deep growth of cells.

Keywords

Melt electrospinning / Near-field direct-writing / Tissue engineering scaffold / Polycaprolactone / Modification

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