Palladium Nanoparticles/Graphdiyne Oxide Nanocomposite with Excellent Peroxidase-like Activity and Its Application for Glutathione Detection

Abstract

In this study, palladium nanoparticles loaded graphdiyne oxide (Pd/GDYO) nanocomposite were fabricated by in-situ reduction of palladium chloride in the dispersion of GDYO, and characterized by Raman spectra, transmission electron microscopy, X-ray diffraction and X-ray photoelectron spectroscopy. The synthesized Pd/GDYO was first found to have catalytic activities similar to those of the peroxidase enzyme, which can catalyze the oxidation of peroxidase substrate 3,3′,5,5′-tetramethylbenzidine(TMB) in the presence of hydrogen peroxide(H₂O₂). Steady-state kinetic studies showed that the catalytic reaction of Pd/GDYO follows a ping-pong mechanism, and Pd/GDYO has a stronger activity to TMB with a Michaelis constant(K _m) value of 5.32×10^-4 mmol/L. Based on the shielding effect of glutathione(GSH) on the Pd/GDYO-H₂O₂-TMB reaction system, a colorimetric detection method for GSH was developed with a wide linear range from 0.1 μmol/L to 40 μmol/L and a limit of detection of 0.1 μmol/L. The method was successfully applied for fast and accurate detection of GSH in injection powder drugs. It was expected that this peroxidase-like Pd/GDYO nanocomposite would have wide applications in the fields of biomedicine and environmental chemistry.

Keywords

Palladium nanoparticle/graphdiyne oxide nanocomposite / Peroxidase-like activity / Colorimetric detection / Glutathione

References

[1] Chen J Z, Gridnev I D. iScience, 2020, 23: 100960.

[2] Liu M, Mou J S, Xu X H, Zhang F F, Xia J F, Wang Z H. Talanta, 2020, 220: 121374.

[3] Zhang Z J, Zhang X H, Liu B W, Liu J W. J. Am. Chem. Soc., 2017, 139: 5412.

[4] Lin X D, Liu Y Q, Tao Z H, Gao J T, Deng J K, Guo T, Yin J J, Wang S. Biosens. Bioelectron., 2017, 94: 471.

[5] Gao L Z, Zhuang J, Nie L, Zhang J B, Zhang Y, Gu N, Wang T H, Feng J, Yang D L, Perrett S, Yan X Y. Nat. Nanotechnol., 2007, 2: 577.

[6] Shi C, Li Y, Gu N. ChemBioChem, 2020, 21: 2722.

[7] Zhang J, Han D, Wang Y H, Wang L W, Chen X Y, Qiao X R, Yu X. Microchim. Acta, 2020, 187: 321.

[8] Liu H, Hua Y, Cai Y Y, Feng L P, Li S, Wang H. Anal. Chim. Acta, 2019, 1092: 57.

[9] Guo Y C, Tian X Q, Wang X F, Sun J. Sensor. Actuat. B: Chem., 2019, 293: 136.

[10] Zhou X B, Wang M K, Chen J Y, Xie X L, Su X G. Anal. Chim. Acta, 2020, 1128: 72.

[11] Zhang X L, Li W M, Zhou Y, Chai Y Q, Yuan R. Biosens. Bioelectron., 2019, 135: 8.

[12] Wang C, Li Y P, Zhou C. Microchim. Acta, 2019, 186: 1.

[13] Hu Q Y, Yang J Y, Zheng Z Y, Ding Y P, Chen Y W, Gao W H. Biosens. Bioelectron., 2019, 143: 111627.

[14] Lan J M, Xu W M, Wan Q P, Zhang X, Lin J, Chen J H, Chen J Z. Anal. Chim. Acta, 2014, 825: 63.

[15] Zhan F K, Yin J J, Zhang A Q, Zhou J X, Wang M L, Jiao T F. Appl. Surf. Sci., 2020, 527: 146719.

[16] Li Z, Liu T, Long J M, Wu Y, Yan B, Ma P, Cao Y. Chin. Chem. Lett., 2019, 30: 582.

[17] Xiao L, Chu W, Sun W J, Xue Y, Jiang C F. Chem. Res. Chinese Universities, 2017, 33(3): 422.

[18] Liu Q, Zhu D B, Guo M L, Yu Y, Cao Y J. Chin. Chem. Lett., 2019, 30: 1639.

[19] Niavol S S, Budde M, Papadogianni A, Heilmann M, Schipani F. Sensor. Actuat. B: Chem., 2020, 325: 128797.

[20] Lan L. Y., Le X. H., Dong H. Y., Xie J., Ying Y. B., Ping J. F., Biosens. Bioelectron., 2020, 165

[21] Dong X, Guo H, Lv C L, Shen G Y. Chem. Bull., 2019, 82: 659.

[22] Yang Z T, Qian J, Yang X W, Jiang D, Du X J, Wang K, Mao H P, Wang K. Biosens. Bioelectron., 2015, 65: 39.

[23] Lin X Y, Wang Y F, Zou M M, Lan T X, Ni Y N. Chin. Chem. Lett., 2019, 30: 1157.

[24] Darabdharaa G, Sharmaa B, Das M R, Boukherroub R, Szunerits S. Sensor. Actuat. B: Chem., 2017, 238: 842.

[25] Ragavana KV, Rastogi N K. Sensor. Actuat. B: Chem., 201, 229: 570.

[26] Liu F P, Tang J Q, Xu J, Shu Y, Xun Q, Wang H M, Hu X Y. Biosens. Bioelectron., 201, 86: 871.

[27] Lin X Q, Deng H H, Wu G W, Peng H P, Liu A L, Lin X H, Xia X H, Chen W. Analyst, 2015, 140: 5251.

[28] Zheng X J, Zhu Q, Song H Q, Zhao X R, Yi T, Chen H L, Chen X G. ACS Appl. Mater. Inter., 2015, 7: 3480.

[29] Zou H Y, Yang T, Lan J, Huang C Z. Anal. Methods, 2017, 9: 841.

[30] Li G X, Li Y L, Liu H B, Guo Y B, Li Y J, Zhu D B. Chem. Commun., 2010, 46: 3256.

[31] Liu J M, Shen X M, Baimanov D, Wang L M, Xiao Y T, Liu H B, Li Y L, Gao X F, Zhao Y L, Chen C Y. ACS. Appl. Mater. Inter., 2019, 11: 2647.

[32] Qi H T, Yu P, Wang Y X, Han G C, Liu H B, Yi Y P, Li Y L, Mao L Q. J. Am. Chem. Soc., 2015, 137: 5260.

[33] Liu R, Gao X, Zhou J Y, Xu H, Li Z Z, Zhang S Q, Xie Z Q, Zhang J, Liu Z F. Adv. Mater., 2017, 29: 1604665.

[34] Ren H, Shao H, Zhang L J, Guo D, Jin Q, Yu R B, Wang L, Li Y L, Wang Y, Zhao H J, Wang D. Adv. Energy Mater., 2015, 5: 1500296.

[35] Chen T M, Xiao J, Yang G W. RSC Adv., 201, 6: 70124.

[36] Xu H H, Deng H H, Chen W, Lin X Q, Wu Y Y, Lin X L, Peng H P, Liu A L, Xia X H, Chen W. Microchim. Acta, 2017, 184: 3945.

[37] Song Y, Qu K, Zhao C, Ren J, Qu X. Adv. Mater., 2010, 22: 2206.

[38] Jiang X, Sun C J, Guo Y, Nie G J, Xu L. Biosens. Bioelectron., 2015, 64: 165.

[39] Wu G W, He S B, Peng H P, Deng H H, Liu A L, Lin X H, Xia X H, Chen W. Anal. Chem., 2014, 86: 10955.

[40] Zhang L N, Deng H H, Lin F L, Xu X W, Weng S H, Liu A L, Lin X H, Xia X H, Chen W. Anal. Chem., 2014, 86: 2711.

[41] Wang Y, Lu J, Tang L H, Chang H X, Li J H. Anal. Chem., 2009, 81: 9710.

[42] Liu X, Wang Q, Zhang Y, Zhang L, Su Y, Lv Y. New J. Chem., 2013, 37: 2174.