A Sensitive Fluorescent Turn-on Probe NapP-deap Based on Naphthalimide Derivative to Detect Hg(II) Ions in HEPES Buffer Solution and Living Cells

Abstract

A highly sensitive fluorescent “turn-on” probe NapP-deap based on naphthalimide derivative was developed that bound Hg²⁺ ions rapidly in the N-2-hydroxyethylpiperazine-N-ethane-sulphonic acid(HEPES) buffer solution via photo-induced electron transfer(PET) being inhibited mechanism. The titration experiment displayed that the emission intensity of NapP-deap at 540 nm was almost linearly increased by about 3-fold. The Job’s plot showed a stoichiometry factor of 1:1 of the ligand-to-metal ratio. The detection limit of fluorescent probe was calculated to be 6.2×10⁻⁹ mol/L. ¹H NMR studies could confirm that one Hg²⁺ ion was bound by the N atoms(a, b) of piperazine or the N atom(c) of pyridine. The fluorescent probe could be used for the detection of Hg²⁺ ions in living cells.

Keywords

Fluorescent “turn-on” / Hg²⁺ / HEPES buffer solution / Living cell / PET mechanism

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