DNA-Silver Nanocluster Binary Probes for Ratiometric Fluorescent Detection of HPV-related DNA

Abstract

Herein, we described a ratiometric strategy based on “chameleon” DNA-silver nano-clusters(DNA-AgNCs) fluorescent binary probes. The strategy was applied to detect high-risk human papillomavirus(HPV) DNA sequences, HPV-16. First, DNA-AgNCs were synthesized by a simple reduction method. The obtained nanoprobes showed typical yellow and red fluorescence of AgNCs. Upon the addition of HPV-16 DNA, the yellow fluorescence of AgNCs was reduced greatly, whereas the red fluorescence of AgNCs was increased. The concentration of HPV-16 DNA in the samples was characterized by the ratio of fluorescence intensity at 570 and 630 nm. The ratiometric nanoprobes showed good selectivity for HPV-16 DNA, and the detection limit was 2 nmol/L. In addition, the practical applicability of this strategy was demonstrated by analysing the HPV-16 DNA in human serum, illustrating its potential promise for clinical diagnosis.

Keywords

DNA-silver nanocluster / Ratiometric / Binary probe / Human papillomavirus

References

[1] Fang B Y, Li C, An J, Zhao S D, Zhuang Z Y, Zhao Y D, Zhang Y X. Nanoscale, 2018, 10(12): 5532.

[2] Bonis-O’Donnell J T D, Thakrar A, Hirschberg J W, Vong D, Queenan B, Fygenson D, Pennathur S. ACS Chemical Neuroscience, 2018, 9(4): 849.

[3] Dong F Y, Feng E D, Zheng T T, Tian Y. ACS Applied Materials and Interfaces, 2018, 10(2): 2051.

[4] Zhang Y, Zhu C F, Zhang L, Tan C L, Yang J, Chen B, Wang L H, Zhang H. Small, 2015, 11(12): 1385.

[5] Miao X M, Cheng Z Y, Ma H Y, Li Z B, Xue N, Wang P. Analytical Chemistry, 2017, 90(2): 1098.

[6] Wu J J, Fu Y, He Z H, Han Y, Zheng L, Zhang J L, Li W. Journal of Physical Chemistry B, 2012, 116(5): 1655.

[7] Obliosca J M, Babin M C, Liu C, Liu Y L, Chen Y A, Batson R A, Ganguly M, Petty J T, Yeh H C. ACS Nano, 2014, 8(10): 10150.

[8] Copp S M, Schultz D, Swasey S M, Faris A, Gwinn E G. Nano Letters, 2016, 16(6): 3594.

[9] Kim H, Kang S Y, Park K S, Park H G. Sensors and Actuators B, 2018, 260: 140.

[10] New S Y, Lee S T, Su X D. Nanoscale, 2016, 8(41): 17729.

[11] Yin B C, Ma J L, Le H N, Wang S L, Xu Z G, Ye B C. Chemical Communications, 2014, 50: 15991.

[12] Li D, Qiao Z Z, Yu Y R, Tang J L, He X X, Shi H, Ye X S, Lei Y L, Wang K M. Chemical Communications, 2018, 54(9): 1089.

[13] Zhang X R, Chen F T, Song X J, He P, Zhang S S. Biosensors and Bioelectronics, 2018, 104: 27.

[14] Zhang S Q, Wang K, Li K B, Shi W, Jia W P, Chen X Y, Sun T, Han D M. Biosensors and Bioelectronics, 2017, 91: 374.

[15] Wang W X, Cheung Y W, Dirkzwager R M, Wong W C, Tanner J A, Li H W, Wu Y Q. Analyst, 2017, 142: 800.

[16] Petty J T, Nicholson D A, Sergev O O, Graham S K. Analytical Chemistry, 2014, 86(18): 9220.

[17] Bonis-O’Donnell J T, Vong D, Pennathu S R, Fygenson D K. Nanoscale, 2016, 8(30): 14489.

[18] MacLean J L, Morishita K, Liu J W. Biosensors and Bioelectronics, 2013, 48(31): 82.

[19] Ge L, Sun X M, Hong Q, Li F. ACS Applied Materials and Interfaces, 2017, 9(37): 32089.

[20] Ge L, Sun X M, Hong Q, Li F. ACS Applied Materials and Interfaces, 2017, 9(15): 13102.

[21] Zhou W J, Dong S J. Chemical Communications, 2017, 53(91): 12290.

[22] Zhou W J, Zhu J B, Fan D Q, Teng Y, Zhu X Q, Dong S J. Advanced Functional Material, 2017, 27(46): 1704092.

[23] Muñoz N M, Bosch F X, Sanjosé S D, Herrero R, Castellsagué X, Shah K V, Snijders P J F, Meijer C J L M. The New England Journal of Medicine, 2003, 348: 518.

[24] Global Burden of Disease Cancer Collaboration. JAMA Oncology, 2015, 1: 505.

[25] Godfroid E, Heinderyckx M, Mansy F, Fayt I, NoëJ l, Thiry L, Bollen A. Journal of Virological Methods, 1998, 75(1): 69.

[26] Jampasa S, Wonsawat W, Rodthongkum N, Siangproh W, Yanatatsaneejit P, Vilaivan T, Chailapakul O. Biosensors and Bioelectronics, 2014, 54: 428.

[27] Wen L, Xu F Z, He X X, Wang K M, He D G, Qing T P, Zou Z. Chinese Journal of Analytical Chemistry, 2015, 43(11): 1620.

[28] Qin S Y, Chen N D, Yang X H, Wang Q, Wang K M, Huang J, Liu J B, Zhou M G. ACS Sensors, 2017, 2(2): 308.