Studies on Surface Properties and Cell Adhesion Properties of BSA Modified DBM Scaffold

Abstract

As a scaffold material for bone tissue engineering, demineralized bone matrix(DBM) has such a limited ability to load cells and growth factors that the surface of the DBM scaffold was modified with bovine serum albumin( BSA) with different concentrations to improve the protein structure and physicochemical properties of the scaffold surface so as to enhance the adhesion of the cells. And the appropriate BSA concentration was explored. Compared with DBM, the scaffold with BSA coating had a smaller pore size and a lower porosity, also, the degradation rate was accelerated and the hydrophilic property was improved. Cells adhesion was observed inside the DBM sca ffold before and after it had been modified, and the BSA modified scaffold had a good cell compatibility. When the concentration of BSA was 20 mg/mL, the adhesion ability of the cells to modified scaffold was significantly increased, and the cell proliferation was facilitated.

Keywords

Scaffold / Demineralized bone matrix / Bovine serum albumin / Cell adhesion

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