DOPA-IGF-1 Coated HA/PLGA Microspheres Promoting Proliferation and Osteoclastic Differentiation of Rabbit Bone Mesenchymal Stem Cells

Abstract

To explore the ability of dihydroxyphenylalanine-insulin-like growth factor-1(DOPA-IGF-1) coated hydroxyapatite/poly(lactic-co-glycolic acid)(HA/PLGA) microspheres to promote the proliferation and osteoclastic differentiation of rabbit bone mesenchymal stem cells(rBMSCs), HA/PLGA microspheres with different HA content (10%, 30%, 50%, mass fraction) were prepared by electrospinning method and HA/PLGA microspheres with 50% HA were coated with IGF-1 and DOPA-IGF-1, respectively. They were co-cultured with rBMSCs, respctively. Cell counting kit-8(CCK-8) detection, confocal laser scanning microscopy(CLSM), alkaline phosphatase(ALP) detection and osteogenesis related genes COL1A1, Runx2 and bone morphogenetic protein-2(BMP-2) detection were conducted to detect the proliferation activity, cell morphology, differentiation ability and the expression level of osteogenesis-related genes of cells cultured on all microspheres groups. The results showed that rBMSCs proliferation increased in an HA content dependent manner, and cells proliferated more in the IGF-1 coated and DOPA-IGF-1 coated groups, in particular in DOPA-IGF-1 coated group, and the differences were more remarkable over time (P<0.05). HA/PLGA microspheres promoted the proliferation and osteogenic differentiation of rBMSCs, and DOPA-IGF-1 coating enhanced the proliferation and osteogenic differentiation of rBMSCs.

Keywords

Biomaterial / Electrospinning / Biocompatibility / Hydrophilic polymer

References

[1] Kalsbeek J H, van Walsum A D P, Vroemen J, Janzing H M J, Winkelhorst J T, Bertelink B P, Roerdink W H. Bone Joint J., 2018, 100-B(4): 443.

[2] Pura J A, Bobyn J D, Tanzer M. Clin. Orthop. Relat. Res., 2016, 474(5): 1224.

[3] King V, Swart A, Winder M J. J. Clin. Neurosci., 2016, 32: 91.

[4] Nguyen T H, Lee B T. Sci. Technol. Adv. Mater., 2012, 13(3): 035002.

[5] Nguyen T H, Lee B T. J. Mater. Sci.: Mater. M., 2010, 21(6): 1969.

[6] Ullah S, Zainol I, Idrus R H. Int. J. Biol. Macromol., 2017, 104: 1020.

[7] Agarwal T, Narayan R, Maji S, Behera S, Kulanthaivel S, Maiti T K, Banerjee I, Pal K, Giri S. Int. J. Biol. Macromol., 2016, 93: 1499.

[8] Shen H, Hu X X, Yang F, Bei J Z, Wang S G. Acta Biomaterialia., 2010, 6(2): 455.

[9] Chung H J, Kim I K, Kim T G, Park T G. Tissue Eng. Pt. A., 2008, 14(5): 607.

[10] Cebatariuniene A, Jarmalaviciute A, Tunaitis V, Puriene A, Venalis A, Pivoriunas A. J. Cranio. Maxill. Surg., 2017, 45(6): 845.

[11] Lee Y S, Lim K S, Oh J E, Yoon A R, Joo W S, Kim H S, Yun C O, Kim S. J. Control. Release, 2015, 205: 128.

[12] Sivandzade F, Mashayekhan S. J. Biomat. Sci. Polym. E., 2018, 29(6): 683.

[13] Kuterbekov M, Machillot P, Lhuissier P, Picart C, Jonas A M, Glinel K. Acta Biomater., 2018, 75: 300.

[14] Yan S, Xia P, Xu S, Zhang K, Li G, Cui L, Yin J. ACS Appl. Mater. Interfaces, 2018, 10(19): 16270.

[15] Gao T, Zhang N, Wang Z, Wang Y, Liu Y, Ito Y, Zhang P. Macromol. Biosci., 2015, 15(8): 1070.

[16] Wang H, Li P, Zhang L, Zhang J, Ling X, Ran H. China Journal of Chinese Materia Medica, 2009, 34(23): 3021.

[17] Singh S K, Vuddanda P R, Singh S, Srivastava A K. Biomed. Research International, 2013, 2013: 909045.

[18] Li C, Qian Y, Zhao S, Yin Y, Li J. Mater. Sci. Eng. C, Mater. Biol. Appl., 2016, 64: 43.

[19] Malik S A, Ng W H, Bowen J, Tang J, Gomez A, Kenyon A J, Day R M. J. Colloid Interf. Sci., 2016, 467: 220.

[20] Fu C, Yang X, Tan S, Song L. Sci. Rep., 2017, 7(1): 12549.

[21] Li Y, Zhang Z, Zhang Z. Cell Tissues Organs, 2018, 205(1): 20.

[22] Mao Z, Li Y, Yang Y, Fang Z, Chen X, Wang Y, Kang J, Qu X, Yuan W, Dai K, Yue B. Front Pharmacol., 2018, 9: 368.

[23] Poudel S B, Bhattarai G, Kook S H, Shin Y J, Kwon T H, Lee S Y, Lee J C. Growth HormIgf Res., 2017, 36: 1.

[24] Rico-Llanos G A, Becerra J, Visser R. J. Biomed. Mater. Res. A., 2017, 105(7): 1867.

[25] Reible B, Schmidmaier G, Moghaddam A, Westhauser F. Int. J. Mol. Sci., 2018, 19(6): 1674.

[26] Shu X L, Feng J, Feng J, Huang X M, Li L Q, Shi Q S. J. Biomater. Appl., 2017, 32(5): 547.

[27] Xian L, Wu X, Pang L, Lou M, Rosen C J, Qiu T, Crane J, Frassica F, Zhang L, Rodriguez J P, Xiaofeng J, Shoshana Y, Shouhong X, Argiris E, Mei W, Xu C. Nat. Med., 2012, 18(7): 1095.

[28] Hawkins-Carranza F G, Munoz-Calvo M T, Martos-Moreno G A, Allo-Miguel G, Del Rio L, Pozo J, Chowen J A, Perez-Jurado L A, Argente J. Horm. Res. Paediat., 2018, 89(3): 200.

[29] Liu C, Li Y, Wang J, Liu C, Liu W, Jian X. Molecules, 2018, 23(7): 1643.

[30] Cho H J, Perikamana S K M, Lee J H, Lee J, Lee K M, Shin C S, Shin H. Acs. Appl. Mater. Inter., 2014, 6(14): 11225.

[31] Wang R, Xu B, Xu H G. Cell Physiol. Biochem., 2017, 41(1): 213.

[32] Shi P J, Wang Q, Yu C P, Fan F J, Liu M, Tu M L, Lu W H, Du M. Colloids Surf. B Biointerfaces, 2017, 155: 477.

[33] Zhou P, Wu J, Xia Y, Yuan Y, Zhang H, Xu S, Lin K. Int. J. Nanomedicine, 2018, 13: 4083.

[34] Mostafa N Z, Uludag H, Varkey M, Dederich D N, Doschak M R, El-Bialy T H. Open Dent. J., 2011, 5: 139.

[35] Zhang G, Na Z, Ren B, Zhao X, Liu W. Int. J. Clin. Exp. Med., 2015, 8(8): 12172.

[36] Reible B, Schmidmaier G, Moghaddam A, Westhauser F. Int. J. Mol. Sci., 2018, 19(6): 1674.

[37] Zhang Z, Li L, Yang W, Cao Y, Shi Y, Li X, Zhang Q. Osteoarthritis Cartilage, 2017, 25(2): 309.

[38] Zhang C, Miyatake H, Wang Y, Inaba T, Wang Y, Zhang P B, Ito Y. Angew. Chem. Int. Edit., 2016, 55(38): 11447.