Double Magnetic Separation-assisted Fluorescence Method for Sensitive Detection of Ochratoxin A

Abstract

A double magnetic separation-assisted fluorescence method was developed to rapidly detect ochratoxin A(OTA). The OTA aptamer functionalized magnetic nanomaterial(Fe₃O₄-Aptamer) and complementary DNA conjugated nitrogen-doped graphene quantum dots(NGQDs-cDNA) were used in this assay. Aptamer could hybridize with cDNA, which induced the NGQDs-cDNA to bind onto Fe₃O₄-Aptamer, and resulted in the fluorescence quenching of NGQDs. After the addition of OTA, the NGQDs-cDNA could release into the solution, and resulted in the recovery of fluorescence signal of NGQDs consequently. By utilizing the magnetic separation, the unbonded NGQDs-cDNA and residual Fe3O4-Aptamer were removed, which significantly increased the fluorescence signal intensity. OTA could be detected in the linear range of 10 nmol/L to 2000 nmol/L, with a limit of detection as 0.66 nmol/L. The advantages of this method include simple operation, good selectivity and high sensitivity, and this method can be used for the rapid detection of ochratoxin A in wheat and corn.

Keywords

Ochratoxin A / Fe₃O₄ / Magnetic separation / Fluorescence method / Aptamer

References

[1] Al-Anati L, Petzinger E. J. Vet. Pharmacol. Ther., 2006, 29: 79.

[2] Meulenberg E P. Toxins(Basel), 2012, 4: 244.

[3] Wu X, Hu J, Zhu B, Lu L, Huang X, Pang D. J. Chromatogr. A, 2011, 1218: 7341.

[4] Bazin I, Nabais E, Lopez-Ferber M. Toxins(Basel), 2010, 2: 2230.

[5] Pfohl-Leszkowicz A, Manderville R A. Mol. Nutr. Food. Res., 2007, 51: 61.

[6] Novo P, Moulas G, Prazeres D M F, Chu V, Conde J P. Sensors Actuators B: Chem., 2013, 176: 232.

[7] Jo E J, Mun H, Kim S J, Shim W B, Kim M G. Food Chem., 2016, 194: 1102.

[8] Pittet A, Royer D. J. Agric. Food Chem., 2002, 50: 243.

[9] Skarkova J, Ostry V, Malir F, Roubal T. Anal. Lett., 2013, 46: 1495.

[10] Zhou J, Xu J J, Huang B F, Cai Z X, Ren Y P. J. Sep. Sci., 2017, 40: 2141.

[11] Mo J, Gong Q, Zhou H, Bai X, Tan J, Peng Z, Huang Y. Journal of Food Safety and Quality, 2016, 7: 182.

[12] Andrade M A, Lanças F M. J. Chromatogr. A, 2017, 1493: 41.

[13] Bougrini M, Baraket A, Jamshaid T, El Aissari A, Bausells J, Za-bala M, El Bari N, Bouchikhi B, Jaffrezic-Renault N, Abdelhamid E. Sensors Actuators B: Chem., 2016, 234: 446.

[14] Karczmarczyk A, Baeumner A J, Feller K H. Electrochimica Acta, 2017, 243: 82.

[15] Zhou J, Rossi J. Nature Reviews Drug Discovery, 2017, 16: 181.

[16] Hamaguchi N, Ellington A, Stanton M. Anal. Biochem., 2001, 294: 126.

[17] Kolpashchikov D M. J. Am. Chem. Soc., 2005, 127: 12442.

[18] Bai H Y, Del Campo F J, Tsai Y C. Biosens. Bioelectron., 2013, 42: 17.

[19] Wang Q Y, Kang Y J. Nanoscale Research Letters, 2016, 11: 150.

[20] Wandtke T, Woźniak J, Kopiński P. Viruses, 2015, 7: 751.

[21] Farokhzad O C, Jon S, Khademhosseini A, Tran T N T, LaVan D A, Langer R. Cancer Res., 2004, 64: 7668.

[22] Shim W B, Mun H, Joung H A, Ofori J A, Chung D H, Kim M G. Food Control, 2014, 36: 30.

[23] Wang C K, Tan R, Chen D. Talanta, 2018, 182: 363.

[24] Goud K Y, Hayat A, Satyanarayana M, Kumar V S, Catanante G, Gobi K V, Marty J L. Microchimica Acta, 2017, 184: 4401.

[25] Lim S Y, Shen W, Gao Z. Chem. Soc. Rev., 2015, 44: 362.

[26] Dey S, Govindaraj A, Biswas K, Rao C. Chem. Phys. Lett., 2014, 595: 203.

[27] Ju J, Chen W. Anal. Chem., 2015, 87: 1903.

[28] Tian Y, Wang F, Liu Y, Pang F, Zhang X. Electrochimica Acta, 2014, 146: 646.

[29] Thanh T D, Balamurugan J, Lee S H, Kim N H, Lee J H. Biosens. Bioelectron., 2016, 81: 259.

[30] Anuar N S, Basirun W J, Ladan M, Shalauddin M, Mehmood M S. Sensors Actuators B: Chem., 2018, 266: 375.

[31] Ju J, Chen W. Biosens. Bioelectron., 2014, 58: 219.

[32] Cai F, Liu X, Liu S, Liu H, Huang Y. RSC Advances, 2014, 4: 52016.

[33] Akin I, Arslan G, Tor A, Ersoz M, Cengeloglu Y. J. Hazard. Mater., 2012, 235: 62.

[34] Xu S, Zhao Y, Zheng F, Zhang Y. Journal of Materials Science, 2016, 51: 2550.

[35] Sun L, Li Y, Sun M, Wang H, Xu S, Zhang C, Yang Q. New J. Chem., 2011, 35: 2697.

[36] Tamer U, Gündoğdu Y, Boyacı İ H H, Pekmez K. J. Nanopart. Res., 2010, 12: 1187.

[37] Yang X, Zhang X, Ma Y, Huang Y, Wang Y, Chen Y. J. Mater. Chem., 2009, 19: 2710.

[38] Kohler N, Sun C, Fichtenholtz A, Gunn J, Fang C, Zhang M. Small, 2006, 2: 785.

[39] Wang S, Zhang Y, Pang G, Zhang Y, Guo S. Anal. Chem., 2017, 89: 1704.

[40] Zhao Y, Li J, Zhao L, Zhang S, Huang Y, Wu X, Wang X. Chem. Eng. J., 2014, 235: 275.

[41] Wang C, Qian J, Wang K, Yang X, Liu Q, Hao N, Wang C, Dong X, Huang X. Biosens. Bioelectron., 2016, 77: 1183.

[42] Li Y, Sun L, Qian J, Wang C, Liu Q, Han E, Hao N, Zhang L, Cai J, Wang K. Anal. Chim. Acta, 2016, 948: 90.

[43] Wang A J, Li Y F, Li Z H, Feng J J, Sun Y L, Chen J R. Materials Science and Engineering: C, 2012, 32: 1640.

[44] Zhu X, Zuo X, Hu R, Xiao X, Liang Y, Nan J. Mater. Chem. Phys., 2014, 147: 963.

[45] Castillo G, Lamberti I, Mosiello L, Hianik T. Electroanalysis, 2012, 24: 512.

[46] Wang C, Dong X, Liu Q, Wang K. Anal. Chim. Acta, 2015, 860: 83.

[47] Al-Taher F, Cappozzo J, Zweigenbaum J, Lee H J, Jackson L, Ryu D. Food Control, 2017, 72: 27.

[48] Soler M, Belushkin A, Cavallini A, Kebbi-Beghdadi C, Greub G, Altug H. Biosens. Bioelectron., 2017, 94: 560.