Spectral Study on the Interactions Among Cu(II), Doxorubicin and CopC

Abstract

Interactions among Cu(II), doxorubicin and CopC have been investigated in detail by means of fluorescence, UV-Vis, IR spectra, isothermal titration calorimetry(ITC) and molecular docking in Tris-HCl buffer(50 mmol/L, pH=7.4, 25 °C). The results suggest that Cu(II)-doxorubicin is formed in a Cu(II) to doxorubicin ratio of 1:2, and the conditional stability constant, K _{[Cu(II)-doxorubicin]} is 1.90×10⁹ L²/mol², CopC and doxorubicin can form a 1:1 complex, the conditional stability constant is greater than 10⁵ L/mol. Binding of doxorubicin causes a conformational change in CopC with the reduction of β-sheet and increase of random coil, and the stability of CopC is decreased. Cu(II), doxorubicin and CopC can form a CopC-Cu(II)-doxorubicin ternary complex. The formation of CopC-Cu(II)-doxorubicin reduced greatly the reduction rate of Cu(II) by ascorbate(Vc), i.e. the binding of doxorubicin affects the action of CopC as redox switch.

Keywords

Doxorubicin / CopC / Cu(II) Interaction

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