Engineering a Floxuridine-integrated RNA Prism as Precise Nanomedicine for Drug Delivery

Abstract

Herein, we report a geometrically well-defined prism assembled by two floxuridine(F) integrated RNA strands as a precise nanomedicine for chemodrug delivery. Owing to the similarity between chemotherapeutic F and uridine(U), all uridines in the component RNA strands are replaced by F during their in vitro transcription syntheses. By specifically designing their sequences, F-containing S-shape tiles with the single-stranded loops are constructed and then further assemble into the RNA prism through T-junction interactions and sticky-end cohesions. The present study demonstrates that the F-integrated RNA prism can serve as an efficient platform for chemodrug delivery and anticancer treatment. We believe that the study would help the future development of RNA-based nanomedicine in various applications.

Keywords

Self-assembly / Floxuridine / RNA prism / Precise nanodrug / Drug delivery

References

[1] Torre L, Bray F, Siegel R, Ferlay J, Lortet-Tieulent J, Jemal A. CA Cancer J. Clin., 2015, 65(2): 87.

[2] Allen T. Cullis P., Adv. Drug Delivery Rev., 2013, 65(1): 36.

[3] Soppimath K, Aminabhavi T, Kulkarni A, Rudzinski W. J. Controlled Release, 2001, 70(1/2): 1.

[4] Lim H, Cho E, Shim J, Kim D, An E, Kim J. J. Colloid Interface. Sci., 2008, 320(2): 460.

[5] Meng E, Zhong Z, Feijen J. Biomacromolecules, 2009, 10: 197.

[6] Garcia-Fuentes M, Alonso M. J. Controlled Release, 2012, 767(2): 496.

[7] Dorniani D, Kura A, Hussein-Al-Ali S, Hussein M, Fakurazi S, Shaari A, Ahmad Z. Sci. World J., 2014, 2014: 416354.

[8] Li Y, Gao G, Lee D. Adv. Healthc. Mater, 2013, 2(3): 388.

[9] Pan G, Jin X, Mou Q, Zhang C. Chin. Chem. Lett, 2017, 28(9): 1822.

[10] Duncan B, Kim C, Rotello V. J. Controlled Release, 2010, 148(1): 122.

[11] Liu Z, Robinson J, Tabakman S, Yang K, Dai H. Mater. Today, 2011, 74(7/8): 316.

[12] Petros R, DeSimone J. Nat. Rev. Drug Discovery, 2010, 9(8): 615.

[13] Keles E, Song Y, Du D, Dong W, Lin Y. Biomater. Sci., 2016, 4(9): 1291.

[14] Lv H, Zhang S, Wang B, Cui S, Yan J. J. Controlled Release, 2006, 774(1): 100.

[15] Zelphati O, Uyechi L, Barron L, Szoka F Jr. Biochim. Biophys. Acta, 1998, 7390(2): 119.

[16] Jong W, Hagens W, Krystek P, Burger M, Sips A, Geertsma R. Biomaterials, 2008, 29(12): 1912.

[17] Wu X, Wu C, Zhang C. Chin. J. Polym. Sci, 2017, 35(1): 1.

[18] Fan C. Chin. Sci. Bull., 2019, 64(10): 987.

[19] Yan J, Hu C, Wang P, Zhao B, Ouyang X, Zhou J, Liu R, He D, Fan C, Song S. Angew. Chem., Int. Ed., 2015, 54(8): 2431.

[20] Pei H, Zuo X, Zhu D, Huang Q, Fan C. Acc. Chem. Res., 2014, 47(2): 550.

[21] You M, Peng L, Shao N, Zhang L, Qiu L, Cui C, Tan W. J. Am. Chem. Soc., 2014, 736(4): 1256.

[22] Hu Q, Li H, Wang L, Gu H, Fan C. Chem. Rev., 2019, 779(10): 6459.

[23] Zhang Q, Jiang Q, Li N, Dai L, Liu Q, Song L, Wang J, Li Y, Tian J, Ding B, Du Y. ACS Nano, 2014, 8(1): 6633.

[24] Li S, Jiang Q, Liu S, Zhang Y, Tian Y, Song C, Wang J, Zou Y, Anderson G, Han J, Chang Y, Liu Y, Zhang C, Chen L, Zhou G, Nie G, Yan H, Ding B, Zhao Y. Nat. Biotechnol, 2018, 36(3): 258.

[25] Lu X, Liu J, Wu X, Ding B. Chem. Asian J., 2019, 74(13): 2193.

[26] Jiang Q, Liu S, Liu J, Wang Z, Ding B. Adv. Mater, 2019, 37(45): 1804785.

[27] Liu J, Song L, Liu S, Jiang Q, Liu Q, Li N, Wang Z, Ding B. Nano Lett., 2018, 18(6): 3328.

[28] Douglas S, Bachelet I, Church G. Science, 2012, 335(6070): 831.

[29] Mou Q, Ma Y, Pan G, Xue B, Yan D, Zhang C, Zhu X Angew. Chem., Int. Ed, 2017, 56(41): 12528.

[30] Ma Y, Liu H, Mou Q, Yan D, Zhu X, Zhang C. Nanoscale, 2018, 70(18): 8367.

[31] Ma Y, Mou Q, Zhu L, Su Y, Jin X, Feng J, Yan D, Zhu X, Zhang C. Chem. Commun., 2019, 55(46): 6603.

[32] Guo Y, Zhang J, Ding F, Pan G, Li J, Feng J, Zhu X, Zhang C. Adv. Mater, 2019, 37(16): 1807533.

[33] Zhang J, Guo Y, Ding F, Pan G, Zhu X, Zhang C Angew Chem. Int. Ed., 2019, 58(39): 13794.

[34] Jasinski D, Haque F, Binzel D, Guo P. ACS Nano, 2017, 77(2): 1142.

[35] Zuo H, Wu S, Li M, Li Y, Jiang W, Mao C. Angew. Chem., Int. Ed., 2015, 54(50): 15118.

[36] Guo P. Nat. Nanotechnol, 2010, 5(12): 833.

[37] Delebecque C, Lindner A, Silver P, Aldaye F. Science, 2011, 333(6041): 470.

[38] Lee J, Hong J, Bonner D, Poon Z, Hammond P. Nat. Mater, 2012, 77(4): 316.

[39] Kruspe S, Hahn U. Angew. Chem., Int. Ed, 2014, 53(39): 10541.

[40] Yu J, Liu Z, Jiang W, Wang G, Mao C. Nat. Commun, 2015, 6(1): 5724.

[41] Choi C, Hao L, Narayan S, Auyeung E, Mirkin C. Proc. Natl., 2013, 770(19): 7625.

[42] Wang P, Rahman M, Zhao Z, Weiss K, Zhang C, Chen Z, Hurwitz S, Shin D, Ke Y. J. Am. Chem. Soc., 2018, 740(7): 2478.

[43] Wang D, Yu C, Xu L, Shi L, Tong G, Wu J, Liu H, Yan D, Zhu X. J. Am. Chem. Soc, 2018, 740(28): 8797.