Facile Route to Enzyme Immobilization: Gloucose Oxidase Entrapped in Titania Under Mild Environmental Conditions and Consequent Electrochemical Sensor Response

Abstract

A facile one-step method of immobilization of the combination of glucose oxidase(GOD) and catalase(CAT) in mesostructured TiO₂ was proposed. Results obtained by transmission electron microspectroscopy and nitrogen adsorption-desorption analysis clearly show that the TiO₂ mediated by the combination of GOD and CAT(CGC) has a large surface area and a narrow pore-size distribution. The CGC immobilized on mesostructured TiO₂ exhibits direct electrochemistry and good electrocatalytic performance without any electron mediator.

Keywords

Direct electrochemistry / Glucose oxidase / Immobilization

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