Chemotransformation of bacterial cells without heat-shock

Abstract

Transformation of DNA into microbial cells via heat-shock approach has been well established in the field of molecular biology for decades. Herein we described an unexpected finding that heat-shock may not play an essential role in the transformation process. This observation was verified via UV-Vis and fluorescence spectroscopies, and confocal microscopy images for various DNAs and bacterial cells. The non-heat-shock approach proposed in this study can be a convenient and beneficial modification for DNA transformation, especially for those laboratories lacking ice machine and heat-shock equipment.

Keywords

Heat-shock / Chemotransformation / DNA / Bacteria

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Jilin University, The Editorial Department of Chemical Research in Chinese Universities and Springer-Verlag GmbH