Influence of K⁺, Na⁺ or Ca²⁺ ions on the interaction between AmB and saturated phospholipids by Langmuir technique

Abstract

Interaction between AmB and cell membrane is influenced by different metal cations. In the presence of K⁺, Na⁺ or Ca²⁺ ions, the surface pressure-area isotherms and the elastic modulus of an amphotericin-dipalmitoyl-phosphatidylcholine(AmB-DPPC) mixed monolayer were discussed. And the excess free energy and entropies of mixing were calculated according to the surface pressure-area isotherms. The phase transition of the mixed monolayer needed a higher concentration of AmB in the sequence Na⁺>pure buffer>K⁺>Ca²⁺. When the molar fraction of AmB(x _AmB) was 0.5, the molecular interaction changed from attraction to repulsion and the mixed monolayer turned to ordered state from disorder state under the induction of K⁺ or Ca²⁺ ions at all surface pressure in our experiment. At high surface pressure, the disorder of monolayer enhanced in the presence of Na+ ions at x _AmB>0.1. At different molar ratios of AmB, the influences of these metal cations were discrepant. These cations may influence AmB molecules to form pores on the monolayer. It is helpful to understand the reduction of AmB’s toxicity as theoretical reference.

Keywords

Amphotericin B / Phase transition / Metal cation / Molecular interaction

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