Studies on the interaction between vanillin and β-Amyloid protein via fluorescence spectroscopy and atomic force microscopy

Abstract

β-Amyloid(Aβ) plaques and intracellular neurofibrillary lesions in the brain are markers of Alzheimer’s disease(AD). The ability to safely decrease Aβ concentrations is potentially important as a preventive strategy for AD. The interactions between vanillin and Aβ polypeptide were investigated via fluorescence spectroscopy and atomic force microscopy(AFM). The results of fluorescence and synchronous spectroscopies illustrate that the intrinsic fluorescence of tyrosine(Tyr) residues in Aβ _1-42 aggregates can be quenched strongly upon the formation of vanillin-Aβ _1-42 complex. Thioflavine T(ThT)-induced fluorescence changes indicated that Aβ _1-42 aggregates could be disaggregated by vanillin, and the AFM images of Aβ _1-42 enunciated the depolymerization of Aβ _1-42 aggregates by vanillin in a dose-dependent manner. Vanillin may be a potential pharmacological agent for the treatment of AD.

Keywords

β-Amyloid fibril / Vanillin / Interaction / Fluorescence spectroscopy / Atomic force microscopy

References

[1] Yan L., Kevin M., Noor K. Int. J. Biochem. Cell Biol., 2003, 35: 1505.

[2] Wang Z., Wan L., Zhou C., Fang X., Wang C., Bai C. Chin. Sci. Bull., 2003, 48: 437.

[3] Hardy J. A., Higgins G. A. Science, 1992, 256: 184.

[4] Selkoe D. J. Neuron., 1991, 6: 487.

[5] Saraiva A. M., Cardoso I., Pereira M. C., Coelho M. A. N., Saraiva M. J., Möhwald H., Brezesinski G. Chem. Bio. Chem., 2010, 11: 1905.

[6] de Felice F. G., Houzel J., Garcia-Abreu J., Louzada P. R. F., Afonso R. C., Meirelles M. N., Lent R., Neto V. M., Ferreira S. T. FASEB J., 2001, 15: 1297.

[7] Gestwicki J. E., Crabtree G. R., Graef I. A. Science, 2004, 306: 865.

[8] McLaurin J., Cecal R., Kierstead M. E., Tian X., Phinney A. L., Manea M., French J. E., Lambermon M. H. L., Darabie A. A., Brown M. E., Janus C., Chishti M. A., Horne P., Westaway D., Fraser P. E., Mount H. T. J., Przybylski M., St George-Hyslop P. Nat. Med., 2002, 8: 1263.

[9] Liu R., Yuan B., Emadi S., Zameer A., Schulz P., McAllister C., Lyubchenko Y., Goud G., Sierks M. R. Biochemistry, 2004, 43: 6959.

[10] Kierstead M., Brown M., McLaurin J. Neurobiol. Aging, 2002, 23(1): 413.

[11] Tjernberg L. O., Naslund J., Lindqvist F., Johannsson J., Karlstrom A. R., Thyberg J., Terenius L., Nordstedt C. J. Biol. Chem., 1996, 271: 8545.

[12] Song S., Wang Y., Xiong L., Qu L., Xu M. Chem. Res. Chinese Universities, 2013, 29(1): 20.

[13] Song S., Wang Y., Xiong L., Qu L., Xu M. Chinese Chem. Lett., 2012, 23: 595.

[14] Yang E. J., Kim S. I., Ku H. Y., Lee D. S., Lee J. W., Kim Y. S., Seong Y. H., Song K. S. Arch. Pharm. Res., 2010, 33(4): 531.

[15] Kim M. J., Lee Y. H., Kwak J. E., Na Y. H., Yoon H. G. BMB Rep., 2011, 44(11): 730.

[16] Song K. S., Jeong W. S., Jun M. Food Sci. Biotechnol., 2012, 21(3): 845.

[17] Kim J., Kim S. H., Lee D. S., Lee D. J., Kim S. H., Chung S., Yang H. O. J. Gins. Res., 2013, 37(1): 100.

[18] Yan J. J., Ahn W. G., Jung J. S., Kim H. S., Hasan M. A., Song D. K. Nutr. Res. Pract., 2014, 8(4): 386.

[19] Choi S. M., Kim B. C., Cho Y. H., Choi K. H., Chang J., Park M. S., Kim M. K., Cho K. H., Kim J. K. Chonnam. Med. J., 2014, 50(2): 45.

[20] Orhan I. E., Daglia M., Nabavi S. F., Loizzo M. R., Sobarzo-Sánchez E., Nabavi S. M. Curr. Med. Chem., 2015, 22: 1004.

[21] Folk D. S., Franz K. J. J. Am. Chem. Soc., 2010, 132: 4994.

[22] Ho K., Yazan L. S., Ismail N., Ismail M. Cancer Epidemiol., 2009, 33: 155.

[23] Ho K., Yazan L. S., Ismail N., Ismail M. Food Chem. Toxicol., 2011, 49: 25.

[24] Chou D. S., Lee J. J., Hsiao G., Hsieh C. Y., Tsai Y. J., Chen T. F., Sheu J. R. J. Agric. Food Chem., 2007, 55: 649.

[25] Abraham D. J., Harris L. S., Meade B. J., Munson A. E., Swerdlow P. S., Patrick G. A. Method of Calming or Sedating an Animal with a Hydroxyl Benzaldehyde Compound, 1997.

[26] Lirdprapamongkol K., Kramb J. P., Suthiphongchai T., Surarit R., Srisomsap C., Dannhardt G., Svasti J. J. Agric. Food Chem., 2009, 57: 3055.

[27] Panisello C., Peña B., Gilabert O. G., Constantí M., Gumí T., Garcia-Valls R. Ind. Eng. Chem. Res., 2013, 52: 9995.

[28] Zhou G., Ruhan A., Ge H., Wang L., Liu M., Wang B., Su H., Yan M., Xi Y., Fan Y. Burns, 2014, 40: 1668.

[29] Xu J., Xu H. Li.u.Y., He H., Li G. Psychiat. Res., 2015, 225: 509.

[30] Zhu M., Han S., Zhou F., Carter S. A., Fink A. L. J. Biol. Chem., 2004, 279: 24452.

[31] Lakowicz J. R. Principles of Fluorescence Spectroscopy, 3rd Ed., 2006, Berlin: Springer Press, 278.

[32] Sharma A., Schulman S. G. Introduction of Fluorescence Spectroscopy, 1999, New York: Wiley, 58.

[33] Lakowica J. R., Weber G. Biochemistry, 1973, 12: 4161.

[34] Ware W. R. J. Phys. Chem., 1962, 66: 455.

[35] Levine H. Protein Sci., 1993, 2: 404.