Voltammetric determination of epinephrine in the presence of uric acid based on aminated graphene and Ag NPs hybrid membrane modified electrode

Abstract

A hybrid membrane consisted of aminated graphene and Ag nanoparticles(Ag NPs) was prepared on the surface of glassy carbon electrode(GCE) by cyclic voltammetry(CV) with aminated graphene(GR-NH2) as matrix for immobilizing Ag NPs. The morphology and electrochemical properties of this hybrid membrane were characterized by scanning electron microscopy(SEM) and CV, respectively, and on this membrane, the voltammetric behaviors of epinephrine(EP) were studied in detail. The membrane exhibited excellent eletro-catalytic activities for the redox of EP, and could resolve the electrochemical response of EP and uric acid(UA) into two oxidation peaks. The peak current of EP was linear with its concentration in the ranges of 0.916–18.3 μmol/L and 18.3–184 μmol/L. The detection limit was 2.0 nmol/L(S/N=3). The proposed modified electrode retained the advantages of easy fabrication, high sensitivity and good repeatability for the determination of EP.

Keywords

Ag nanoparticle(Ag NP) / Aminated graphene(GR-NH2) / Epinephrine(EP) / Uric acid(UA) / Cyclic voltammetry(CV)

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