Preparation and investigation of arsenic trioxide-loaded polylactic acid/magnetic hybrid nanoparticles

Abstract

In order to improve the therapeutic index and reduce the side effects of As₂O₃ so as to expand its clinical utility to solid tumors, As₂O₃-loaded polylactic acid(PLA)/magnetic nanoparticles(PAMNs) were prepared and the preparation conditions were optimized by the improved double emulsion solvent-evaporation method to obtain as a drug delivery system with a high As₂O₃ encapsulation efficiency and homogeneous nanosized particles. Successful loading of magnetic nanoparticles into the nanoparticles was confirmed by Fourier transform infrared(FTIR) spectroscopy. The drug loading, encapsulation efficiency and releasing properties of As₂O₃ in PAMNs were investigated by atomic fluorescence spectrometer analysis. Subsequently, the in vitro antitumor efficacy of free As₂O₃ and As₂O₃-PAMNs against SMMC-7721 cell line was investigated. Finally, confocal fluorescence microscopy was conducted to reveal the celluar uptake of As₂O₃-loaded PAMNs by SMMC-7721 cell line.

Keywords

As₂O₃ / Magnetism / Polylactic acid nanoparticle / Drug release

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