Ultrasound-assisted extraction of kamebakaurin from Rabdosia excisa by response surface methodology

Abstract

For the efficient extraction of kamebakaurin(KA), the ultrasound-assisted extraction(UAE) of KA from Rabdosia excisa(R. excisa) via response surface methodology(RSM) was investigated with high-performance liquid chromatography(HPLC). Effects of the experimental parameters such as extraction solvent, ratio of liquid to plant material, extraction time and extraction temperature on the extracting efficiency of KA from R. excisa were evaluated, and the purity of KA in residual was calculated. The optimized conditions were 65.5%(volume fraction) acetone, 35 °C, time of 24.6 min with ultrasound of 80 W/L, 40 kHz, ratio of liquid to plant material at 30:1(mL/g). The maximum yield of KA is 0.708 mg/kg, with mean purity of 6.09%, indicating that ultrasound-assisted extraction is a feasible and useful method for extracting KA from R. excisa.

Keywords

Ultrasound-assisted extraction / Kamebakaurin / Rabdosia excisa / Response surface methodology

References

[1] Editorial Board of Flora of China of Chinese Academy of Sciences. Flora of China, 1977, Beijing: Science Press 490.

[2] Yan Z K, Li W L. Flora of Medicinal Plants in Changbai Mountain, 1996, Beijing: People’s Medical Publishing House 55.

[3] Gui M Y, Aoyagi Y, Jin Y R, Li X W, Hasuda T, Takeya K. J. Nat. Prod., 2004, 67: 373.

[4] Sun H D, Huang S X, Han Q B. Nat. Prod. Rep., 2006, 23: 673.

[5] Zhu X H. Investigation on the Chemical and Bioactive Constituents of Isodon Excisa, 2010, Shanghai: East China Normal University.

[6] Piao Y. The Chemistry Research of Bioactive Constituents of Isodon excia in Changbai Mountain, 2011, Yanbian: Yanbian University.

[7] Lee J H, Choi J K, Noh M S, Hwang B Y, Hong Y S, Lee J J. Planta Med., 2004, 70: 526.

[8] Kim B W, Koppula S, Kim I S, Lim H W, Hong S M, Han S D, Hwang B Y, Choi D K. J. Pharmacol. Sci., 2011, 116: 296.

[9] Hwang B Y, Lee J H, Koo T H, Kim H S, Hong Y S, Ro J S, Lee K S, Lee J J. Planta Med., 2001, 67: 406.

[10] Lee J H, Koo T H, Hwang B Y, Lee J J. J. Biolog. Chem., 2002, 277: 18411.

[11] Paris D, Patel N, Quadros A, Linan M, Bakshi P, Ait-Ghezala G, Mullan M. Neurosci. Lett., 2007, 415: 11.

[12] Zhao Q. C., Hao Y. F., Nan M. L., Li Q. J., Zhao X. J., New Preparing Method of Total Diterpenoids from Rabdosia excisa, CN201010262110.7, 2010

[13] Wang Z B, Zhang J, Wang S Q, Ma H L, Wang Q Q, Chen X Y. Trans. Chin. Soc. Agri. Mach., 2011, 42: 150.

[14] Vinatoru M. Ultrason. Sonochem., 2001, 8: 303.

[15] Li Y W, Jiang G C, Zhang C G, Wei W L. Chem. J. Chinese Universities, 2012, 33(1): 66.

[16] Sun H D, Xu Y L, Jiang B. Diterpenoids from Isodon Species, 2001, Beijing: Science Press 19.

[17] Kim S J, Murthy H N, Hahn E J, Lee H L, Paek K Y. Sep. Purif. Technol., 2007, 56: 401.