Determination of amino acids in Panax notoginseng by microwave hydrolysis and derivatization coupled with capillary zone electrophoresis detection

Abstract

The microwave hydrolysis and derivatization coupled with capillary electrophoresis detection were developed for the separation and determination of the amino acids in Panax notoginseng. The experimental conditions for the microwave hydrolysis and derivatization were examined and optimized. Several parameters of capillary electrophoresis, such as pH value of background electrolyte, borate concentration and applied voltage were optimized. Under the selected conditions, 11 amino acids were completely separated. The real sample was analyzed and the results were satisfactory. Compared with that of conventional heat hydrolysis and derivatization, the analytical time of this method was significantly shortened.

Keywords

Microwave hydrolysis / Derivatization / Amino acid / Capillary electrophoresis / Panax notoginseng / 2,4-Dinitrofluorobenene

References

[1] Wang C Z, McEntee E, Wicks S, Wu J A, Yuan C S. J. Nat. Med., 2006, 60(2): 97.

[2] Xu Q F, Fang X L, Chen D F. J. Ethnopharmacol, 2003, 84: 187.

[3] Wu J L, Wei H D, Sui X F, Lin J, Wang T Y, Fen G Q, Xue J. Bull. Environ. Contarn. Toxicol., 2010, 84: 469.

[4] Garcia A, Olmo B, Lopez-Gonzalvez A, Cornejo L, Ruperez F J, Barbas C. J. Pharm. Biomed. Anal., 2008, 46(2): 356.

[5] Ewing A G, Wallingford R A, Olefirowicz T M. Anal. Chem., 1989, 61(4): 275A.

[6] Li D, Wang Z M, Wang L, Qu C L, Zhang H Q. Chromatogr., 2009, 70: 825.

[7] Li H J, Liu X Q, Niu W X, Zhu S Y, Fan L S, Shi L H, Xu G B. Electrophoresis, 2008, 29(22): 475.

[8] Tagliaro F, Manettoa G, Crivellentea F, Smithb F P. Forensic. Sci. Int., 1998, 92: 75.

[9] Sun J Y, Xu X Y, Yu H, You T Y. Chem. Res. Chinese Universities, 2012, 28(3): 415.

[10] Yu C X, Yuan B Q, You T Y. Chem. Res. Chinese Universities, 2011, 27(1): 34.

[11] Wang T, Yang X B, Wang D Y, Jiao Y D, Wang Y, Zhao Y. Carbohydr. Polym., 2012, 88: 754.

[12] D’Eramo F, Marioli J M, Arévalo A H. Talanta, 2003, 61: 341.

[13] Larraín M A, Abugoch L, Quitral V, Vinagre J, Segovia C. Food Chem., 2002, 76: 377.

[14] Wang T L, Zhao S S, Shen C H, Tang J, Wang D. Food Chem., 2009, 112(1): 215.

[15] Rubinstein I, Bard A J. J. Am. Chem. Soc., 1980, 102: 6641.

[16] Lin L, Li S H, Jiang R H. Chem. Res. Chinese Universities, 2011, 27(4): 589.

[17] Sun Y, Huang D W, Li X H, Hu J E, Xiu Z L. Chem. Res. Chinese Universities, 2012, 28(1): 108.

[18] Zhou L, Yan N, Zhang H G, Zhou X M, Pu Q S, Hu Z D. Talanta, 2010, 82: 72.

[19] Wang Y T, You J Y, Yu Y, Qu C L, Zhang H R, Ding L, Zhang H Q, Li X W. Food Chem., 2008, 110: 161.

[20] Zhu S D, Wu Y X, Yu Z N, Zhang X, Wang C W, Yu F Q, Jin S W. Bioresource Technol., 2006, 97: 1964.

[21] Messia M C, Falco T D, Panfili G, Marconi E. Meat Sci., 2008, 80: 401.

[22] Lee J H, Choung M G. Food Chem., 2011, 129: 577.

[23] Strassnig S, Gfrerer M, Lankmayr E P. J. Chromatogr. B, 2004, 813: 151.

[24] Kouremenos K, Harynuk J J, Winniford W L, Morrison P D, Marriott P J. J. Chromatogr. B, 2010, 878: 1761.

[25] Derksen C, Walker A, Goodison B. Remote Sens. Environ., 2003, 88: 271.

[26] Jgrgensen N O G, Jensen R E. Mar. Chem., 1997, 57: 287.

[27] Zhang X, Yang L, Mester Z. Anal. Chim. Acta, 2012, 744: 54.

[28] Stenberg M, Marko-Varga G, Oste R. Food Chem., 2001, 774: 21.

[29] Kabaha K, Taralp A, Cakmak I, Ozturk L. J. Agric. Food Chem., 2011, 59: 2958.

[30] Li M J, You J Y, Yao S, Ding L, Liu Z Y, Zhang H Q. Chem. Res. Chinese Universities, 2004, 20(6): 703.

[31] Schwer C, Kenndler E. Anal. Chem., 1991, 63: 1801.

[32] Kopec S, Holzgrabe U. Electrophoresis, 2007, 28: 2154.