Immobilization of hemoglobin on cobalt nanoparticles-modified indium tin oxide electrode: Direct electrochemistry and electrocatalytic activity

Abstract

A cobalt nanoparticles-attached indium tin oxide(CoNPs/ITO) electrode was applied to the immobilization of hemoglobin(Hb) and an Hb modified CoNPs/ITO electrode(Hb/CoNPs/ITO) was prepared. The direct electron transfer of Hb was shown by the well-behaved voltammetric responses for Hb/CoNPs/ITO electrode and the effects of scan rate and pH value were observed. Based on the catalytic activity of Hb immobilized on the CoNPs/ITO electrode toward the reduction of H₂O₂, a mediator-free H₂O₂ sensor was developed. A linear relationship existed between the catalytic current and the H₂O₂ concentration in a range of 1.0–100.0 μmol/L with a detection limit (S/N=3) of 0.2 μmol/L.

Keywords

Cobalt nanoparticle / Indium tin oxide electrode / Direct electron transfer / Hemoglobin / Electrocatalysis; Hydrogen peroxide

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