Fabrication of a magnetite nanoparticle-loaded polymeric nanoplatform for magnetically guided drug delivery

Abstract

We developed a magnetite nanoparticle-loaded polymeric nanoplatform for magnetically guided 10-hydroxycamptothecin(HCPT) delivery. The nanoplatform was fabricated by simultaneously incorporating magnetite nanoparticles(NPs) and HCPT into the polymer micelle self-assembled from methoxy polyethylene glycol-poly(D,L-lactide-co-glycolide)(MPEG-PLGA) copolymer. Successful loading of HCPT into the nanoplatform was confirmed by Fourier transform infrared(FTIR) spectroscopy. Subsequently, we examined the in vitro antitumor efficacy of free HCPT and nanoplatform against three different cancer cell lines—HeLa, A549 and HepG2. Flow cytometric analysis was conducted to reveal the cell apoptosis caused by free HCPT and nanoplatform. Finally, the magnetic targeting property of the nanoplatform was evaluated by a self-designed in vitro experiment.

Keywords

Magnetite nanoparticle / 10-Hydroxycamptothecin(HCPT) / Nanoplatform / Magnetic targeting

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