ZFP541 and KCTD19 regulate chromatin organization and transcription programs for male meiotic progression
Xu Zhou , Kailun Fang , Yanlei Liu , Weidong Li , Yingjin Tan , Jiaming Zhang , Xiaoxia Yu , Guoqiang Wang , Yanan Zhang , Yongliang Shang , Liangran Zhang , Charlie Degui Chen , Shunxin Wang
Cell Proliferation ›› 2024, Vol. 57 ›› Issue (4) : e13567
ZFP541 and KCTD19 regulate chromatin organization and transcription programs for male meiotic progression
The successful progression of meiosis prophase I requires integrating information from the structural and molecular levels. In this study, we show that ZFP541 and KCTD19 work in the same genetic pathway to regulate the progression of male meiosis and thus fertility. The Zfp541 and/or Kctd19 knockout male mice show various structural and recombination defects including detached chromosome ends, aberrant localization of chromosome axis components and recombination proteins, and globally altered histone modifications. Further analyses on RNA-seq, ChIP-seq, and ATAC-seq data provide molecular evidence for the above defects and reveal that ZFP541/KCTD19 activates the expression of many genes by repressing several major transcription repressors. More importantly, we reveal an unexpected role of ZFP541/KCTD19 in directly modulating chromatin organization. These results suggest that ZFP541/KCTD19 simultaneously regulates the transcription cascade and chromatin organization to ensure the coordinated progression of multiple events at chromosome structural and biochemical levels during meiosis prophase I.
2023 The Authors. Cell Proliferation published by Beijing Institute for Stem Cell and Regenerative Medicine and John Wiley & Sons Ltd.
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