DNA Synthesis and Assembly Technologies: From Oligonucleotides to Complete Genomes

Wei Tan; Xuemei Jia; Ying Liu; Chi Yao; Dayong Yang

doi:10.1002/cjoc.202401134

Chinese Journal of Chemistry ›› 2025, Vol. 43 ›› Issue (16) : 2069 -2081. DOI: 10.1002/cjoc.202401134

Critical Review

DNA Synthesis and Assembly Technologies: From Oligonucleotides to Complete Genomes

Wei Tan ¹^,²
, Xuemei Jia ²
, Ying Liu ²
, Chi Yao ¹^,²
, Dayong Yang ¹^,²

Author information +

History +

PDF

Abstract

DNA synthesis and assembly technology is the fundamental enabling technology of synthetic biology, providing methods for humans to understand and modify organisms. Oligonucleotide chains and long DNA fragments synthesized through DNA synthesis and assembly technology are becoming increasingly widely used in fields such as biomedicine, energy, new materials, and information storage, with strong application prospects. This review provides a comprehensive and systematic introduction and exposition of current DNA synthesis and assembly technologies, discussing current challenges and future research prospects.

Keywords

DNA synthesis / DNA assembly / Synthetic biology / Synthetic genomics / DNA / Biosynthesis / Green chemistry

Cite this article

Download citation ▾

Wei Tan, Xuemei Jia, Ying Liu, Chi Yao, Dayong Yang. DNA Synthesis and Assembly Technologies: From Oligonucleotides to Complete Genomes. Chinese Journal of Chemistry, 2025, 43(16): 2069-2081 DOI:10.1002/cjoc.202401134