Characterization of HpnG as a Purine Nucleoside Phosphorylase in Bacteriohopanepolyol Biosynthesis

Xinhui Li; Xiaoyu Zhu; Yuting Zhong; Wei Zhang; Fener Chen; Wenning Wang; Wei Ding; Qi Zhang

doi:10.1002/cjoc.202400233

Chinese Journal of Chemistry ›› 2024, Vol. 42 ›› Issue (19) : 2351 -2356. DOI: 10.1002/cjoc.202400233

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Characterization of HpnG as a Purine Nucleoside Phosphorylase in Bacteriohopanepolyol Biosynthesis

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Abstract

HpnG plays a crucial role in the production of ribosylhopane, a key intermediate in the biosynthesis of bacteriohopanepolyol. Despite early extensive studies, the precise function of HpnG has remained elusive. Here, we report functional characterization of HpnG as a purine nucleoside phosphorylase, which converts adenosylhopane to phosphoribosylhopane in the presence of phosphate. HpnG demonstrates broad substrate specificity and impressive stability, making it a valuable enzymatic tool for applications in nucleoside processing and related biotechnology.

Keywords

Enzymes / Enzyme catalysis / Nucleosides / Biosynthesis / Natural products / Lipids / Phosphorolysis / Biotransformations

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Xinhui Li, Xiaoyu Zhu, Yuting Zhong, Wei Zhang, Fener Chen, Wenning Wang, Wei Ding, Qi Zhang. Characterization of HpnG as a Purine Nucleoside Phosphorylase in Bacteriohopanepolyol Biosynthesis. Chinese Journal of Chemistry, 2024, 42(19): 2351-2356 DOI:10.1002/cjoc.202400233