Interfacing DNA and Aptamers with Gold Nanoparticles: From Fundamental Colloid and Interface Sciences to Biosensors

Abstract

Interfacing DNA oligonucleotides and DNA aptamers with gold nanoparticles has generated numerous functional hybrid materials for sensing, self-assembly and drug delivery applications. Our lab has been working in this area for 15 years. In this article, the current understanding of the adsorption of DNA to gold nanoparticles is summarized, and related applications in bioconjugation of DNA to gold surface is described. In addition, problems of using gold nanoparticles to signaling aptamer binding are discussed. Finally, re-selection of aptamers for previously reported targets using the library-immobilization method is reviewed.

Keywords

Aptamers / Biosensors / Gold nanoparticles / SELEX / Bioconjugation

References

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