Collagen-based materials in male genitourinary diseases and tissue regeneration

Abstract

Male genitourinary dysfunction causes serious physical or mental distress, such as infertility and psychological harm, which leads to impaired quality of life. Current conventional treatments involving drug therapy, surgical repair, and tissue grafting have a limited effect on recovering the function and fertility of the genitourinary organs. To address these limitations, various biomaterials have been explored, with collagen-based materials increasingly gaining attention for reconstructing the male genitourinary system due to their superior biocompatibility, biodegradability, low antigenicity, biomimetic 3D matrix characteristics, hemostatic efficacy, and tissue regeneration capabilities. This review covers the recent biomedical applications of collagen-based materials including treatment of erectile dysfunction, premature ejaculation, penile girth enlargement, prostate cancer, Peyronie's disease, chronic kidney disease, etc. Although there are relatively few clinical trials, the promising results of the existing studies on animal models reveal a bright future for collagen-based materials in the treatment of male genitourinary diseases.

Graphic Abstract

References

[1] LiuX, ZhengC, LuoX, WangX, JiangH. Recent advances of collagen-based biomaterials: multi-hierarchical structure, modification and biomedical applications. Mater Sci Eng C Mater Biol Appl, 2019, 99: 1509-1522

[2] SongJE, ThangaveluM, ChoiJ, ChoH, MoonBK, YoonSJ, et al. . Bone regeneration using duck's feet-derived collagen scaffold as an alternative collagen source. Adv Exp Med Biol, 2020, 1250: 3-13

[3] RadaelliF, D'AlfonsoL, ColliniM, MingozziF, MarongiuL, GranucciF, et al. . μMAPPS: a novel phasor approach to second harmonic analysis for in vitro-in vivo investigation of collagen microstructure. Sci Rep, 2017, 7(1): 17468

[4] Ricard-BlumS. The collagen family. Cold Spring Harb Perspect Biol, 2011, 3(1): a004978

[5] SöderhällC, MarenholzI, KerscherT, RüschendorfF, Esparza-GordilloJ, WormM, et al. . Variants in a novel epidermal collagen gene (COL29A1) are associated with atopic dermatitis. PLoS Biol, 2007, 5(9): e242

[6] HirataY, MatsuiY, WadaI, HosokawaN. Endoplasmic reticulum–to–Golgi trafficking of procollagen III via conventional vesicular and tubular carriers. Mol Biol Cell, 2022

[7] TimplR. Structure and biological activity of basement membrane proteins. Eur J Biochem, 1989, 180(3): 487-502

[8] Rezvani GhomiE, NourbakhshN, Akbari KenariM, ZareM, RamakrishnaS. Collagen-based biomaterials for biomedical applications. J Biomed Mater Res B Appl Biomater, 2021, 109(12): 1986-1999

[9] Geanaliu-NicolaeRE, AndronescuE. Blended natural support materials-collagen based hydrogels used in biomedicine. Materials (Basel), 2020, 13(24): 5641

[10] LiuGY, AgarwalR, KoKR, RuthvenM, SarhanHT, FramptonJP. Templated assembly of collagen fibers directs cell growth in 2D and 3D. Sci Rep, 2017, 7: 9628

[11] BatistaMP, FernándezN, GasparFB, BronzeMDR, DuarteARC. Extraction of biocompatible collagen from blue shark skins through the conventional extraction process intensification using natural deep eutectic solvents. Front Chem, 2022, 10: 937036

[12] YarahmadiA, DoustiB, Karami-KhorramabadiM, AfkhamiH. Materials based on biodegradable polymers chitosan/gelatin: a review of potential applications. Front Bioeng Biotechnol, 2024, 12: 1397668

[13] Garcia-GarciaA, Muñana-GonzálezS, Lanceros-MendezS, Ruiz-RubioL, AlvarezLP, Vilas-VilelaJL. Biodegradable natural hydrogels for tissue engineering, controlled release, and soil remediation. Polymers (Basel), 2024, 16(18): 2599

[14] JiY, DouYN, ZhaoQW, ZhangJZ, YangY, WangT, et al. . Paeoniflorin suppresses TGF-β mediated epithelial-mesenchymal transition in pulmonary fibrosis through a Smad-dependent pathway. Acta Pharmacol Sin, 2016, 37(6): 794-804

[15] WolfMT, VodovotzY, TotteyS, BrownBN, BadylakSF. Predicting in vivo responses to biomaterials via combined in vitro and in silico analysis. Tissue Eng Part C Methods, 2015, 21(2): 148-159

[16] YariD, EbrahimzadehMH, MovaffaghJ, ShahroodiA, ShirzadM, QujeqD, et al. . Biochemical aspects of scaffolds for cartilage tissue engineering; from basic science to regenerative medicine. Arch Bone Jt Surg, 2022, 10(3): 229-244

[17] GattazzoF, UrciuoloA, BonaldoP. Extracellular matrix: a dynamic microenvironment for stem cell niche. Biochim Biophys Acta, 2014, 1840(8): 2506-2519

[18] Martínez-PuigD, Costa-LarriónE, Rubio-RodríguezN, Gálvez-MartínP. Collagen supplementation for joint health: the link between composition and scientific knowledge. Nutrients, 2023, 15(6): 1332

[19] ZhaoC, XiaoY, LingS, PeiY, RenJ. Structure of Collagen. Methods Mol Biol, 2021, 2347: 17-25

[20] Wosicka-FrąckowiakH, PoniedziałekK, WoźnyS, KuprianowiczM, NygaM, JadachB, MilanowskiB. Collagen and its derivatives serving biomedical purposes: a review. Polymers, 2024, 16(18): 2668

[21] RatherJA, MajidSD, DarAH, AminT, MakrooHA, MirSA, et al. . Extraction of gelatin from poultry byproduct: influence of drying method on structural, thermal, functional, and rheological characteristics of the dried gelatin powder. Front Nutr, 2022, 9: 895197

[22] Casas-ForeroN, Orellana-PalmaP, PetzoldG. Comparative study of the structural properties, color, bioactive compounds content and antioxidant capacity of aerated gelatin gels enriched with cryoconcentrated blueberry juice during storage. Polymers (Basel), 2020, 12(12): 2769

[23] El-MeligyMA, ValachováK, JuránekI, TamerTM, ŠoltésL. Preparation and physicochemical characterization of gelatin-aldehyde derivatives. Molecules, 2022, 27(20): 7003

[24] BaoL, LiC, TangM, ChenL, HongFF. Potential of a composite conduit with bacterial nanocellulose and fish gelatin for application as small-diameter artificial blood vessel. Polymers (Basel), 2022, 14(20): 4367

[25] ZhangY, DaiJ, YanL, SunY. Intra-articular injection of decellularized extracellular matrices in the treatment of osteoarthritis in rabbits. PeerJ, 2020, 8: e8972

[26] BadylakSF, FreytesDO, GilbertTW. Extracellular matrix as a biological scaffold material: structure and function. Acta Biomater, 2009, 5(1): 1-13

[27] WangX, ChenG, HuangC, TuH, ZouJ, YanJ. Bone marrow stem cells-derived extracellular matrix is a promising material. Oncotarget, 2017, 8(58): 98336-98347

[28] KimYH, ShimHS, LeeJ, KimSW. A prospective randomized controlled multicenter clinical trial comparing paste-type acellular dermal matrix to standard care for the treatment of chronic wounds. J Clin Med, 2022, 11(8): 2203

[29] ZhouW, ZhaoX, ShiX, ChenC, CaoY, LiuJ. Constructing tissue-engineered dressing membranes with adipose-derived stem cells and acellular dermal matrix for diabetic wound healing: a comparative study of hypoxia- or normoxia-culture modes. Stem Cells Int, 2022, 2022: 2976185

[30] BullockN, EllulT, BennettA, SteggallM, BrownG. The 100 most influential manuscripts in andrology: a bibliometric analysis. Basic Clin Androl, 2018, 28: 15

[31] LiMK, GarciaLA, RosenR. Lower urinary tract symptoms and male sexual dysfunction in Asia: a survey of ageing men from five Asian countries. BJU Int, 2005, 96(9): 1339-1354

[32] EladakS, MoisonD, GuerquinMJ, MatilionyteG, KilcoyneK, N'Tumba-BynT, et al. . Effects of environmental bisphenol a exposures on germ cell development and Leydig cell function in the human fetal testis. PLoS ONE, 2018, 13(1): e0191934

[33] GBD 2021 Fertility and Forecasting Collaborators. Global fertility in 204 countries and territories, 1950–2021, with forecasts to 2100: a comprehensive demographic analysis for the Global Burden of Disease Study 2021. Lancet. 2024; 403(10440): 2057–99. https://doi.org/10.1016/S0140-6736(24)00550-6

[34] JinXX, XuYF, YingX, QianYQ, JinPZ, DongMY. Clinical application of noninvasive prenatal testing for pregnant women with assisted reproductive pregnancy. Int J Womens Health, 2021, 13: 1167-1174

[35] Dehdari EbrahimiN, ParsaS, NozariF, ShahlaeeMA, MaktabiA, SayadiM, et al. . Protective effects of melatonin against the toxic effects of environmental pollutants and heavy metals on testicular tissue: a systematic review and meta-analysis of animal studies. Front Endocrinol (Lausanne), 2023, 14: 1119553

[36] AlmujaydilMS. The role of dietary nutrients in male infertility: a review. Life (Basel), 2023, 13(2): 519

[37] AgarwalA, MulgundA, HamadaA, ChyatteMR. A unique view on male infertility around the globe. Reprod Biol Endocrinol, 2015, 13: 37

[38] YangJ, LinD, YaoW, YunD, ZhouL, GaoS, SunF. NBMA promotes spermatogenesis by mediating Oct4 pathway. ChemistryOpen, 2022, 11(3): e202100219

[39] LiY, SuY, ZhouT, HuZ, WeiJ, WangW, et al. . Activation of the NLRP3 inflammasome pathway by prokineticin 2 in testicular macrophages of uropathogenic escherichia coli- induced orchitis. Front Immunol, 2019, 10: 1872

[40] LottiF, MaggiM. Sexual dysfunction and male infertility. Nat Rev Urol, 2018, 15(5): 287-307

[41] BurnettAL, NehraA, BreauRH, CulkinDJ, FaradayMM, HakimLS, et al. . Erectile dysfunction: AUA guideline. J Urol, 2018, 200(3): 633-641

[42] LewisRW, Fugl-MeyerKS, CoronaG, HayesRD, LaumannEO, MoreiraED Jr, et al. . Definitions/epidemiology/risk factors for sexual dysfunction. J Sex Med, 2010, 7(4 Pt 2): 1598-1607

[43] ShamloulR, GhanemH. Erectile dysfunction. Lancet, 2013, 381(9861): 153-165

[44] HargreaveTB. Genetic basis of male fertility. Br Med Bull, 2000, 56(3): 650-671

[45] DaneshmandpourY, BahmanpourZ, HamzeiyH, Mazaheri MoghaddamM, Mazaheri MoghaddamM, KhademiB, et al. . MicroRNAs association with azoospermia, oligospermia, asthenozoospermia, and teratozoospermia: a systematic review. J Assist Reprod Genet, 2020, 37(4): 763-775

[46] FangY, SuY, XuJ, HuZ, ZhaoK, LiuC, et al. . Varicocele-Mediated Male Infertility: from the perspective of Testicular Immunity and Inflammation. Front Immunol, 2021, 12: 729539

[47] LewisSEM. Revisiting the impact of varicocele and its treatments on male fertility. Reprod Biomed Online, 2022, 45(6): 1061-1063

[48] MaJ, LiC, QianH, ZhangY. MTA1: a vital modulator in prostate cancer. Curr Protein Pept Sci, 2022, 23(7): 456-464

[49] SanchezE, PastuszakAW, KheraM. Erectile dysfunction, metabolic syndrome, and cardiovascular risks: facts and controversies. Transl Androl Urol., 2017, 6(1): 28-36

[50] WangC, ZhangH, LiuZ, TuX, ZhangY. A modified procedure to diagnose erectile dysfunction using the international index of erectile function (IIEF-6) combined with the premature ejaculation diagnosis tool (PEDT) via an internet survey. Sex Med, 2022, 10(3): 100506

[51] HuangT, WangG, HuY, ShiH, WangK, YinL, et al. . Structural and functional abnormalities of penile cavernous endothelial cells result in erectile dysfunction at experimental autoimmune prostatitis rat. J Inflamm (Lond), 2019, 16: 20

[52] RomanowskaM, OknińskiT, PawlakJ. Hybrid technique for postoperative ventral hernias - own experience. Wideochir Inne Tech Maloinwazyjne, 2016, 10(4): 534-540

[53] LiX, ZhangX, HaoM, WangD, JiangZ, SunL, et al. . The application of collagen in the repair of peripheral nerve defect. Front Bioeng Biotechnol, 2022, 10: 973301

[54] AubertL, DubusM, RammalH, BourC, MongaretC, Boulagnon-RombiC, et al. . Collagen-based medical device as a stem cell carrier for regenerative medicine. Int J Mol Sci, 2017, 18(10): 2210

[55] WangQ, ChenX, RenC, XuZ, PanY, WangS, et al. . Combination of mesenchymal stem cells carried on artificial extracellular matrix with LIPUS is a potential therapy that may cure diabetes mellitus erectile dysfunction. Med Hypotheses, 2023, 180: 111164

[56] ArunA, MalrautuP, LahaA, LuoH, RamakrishnaS. Collagen nanoparticles in drug delivery systems and tissue engineering. Appl Sci, 2021, 11(23): 11369

[57] MoonHW, KimIG, KimMY, JungAR, ParkK, LeeJY. erectile dysfunction treatment using stem cell delivery patch in a cavernous nerve injury rat model. Bioengineering (Basel), 2023, 10(6): 635

[58] AndersonD, LaforgeJ, RossMM, VanlangendonckR, HasoonJ, ViswanathO, et al. . Male sexual dysfunction. Health Psychol Res, 2022, 10(3): 37533

[59] SubhanF, HussainZ, TauseefI, ShehzadA, WahidF. A review on recent advances and applications of fish collagen. Crit Rev Food Sci Nutr, 2021, 61(6): 1027-1037

[60] BillingsC, BowAJ, NewbySD, DonnellRL, DharM, AndersonDE. Effects on tissue integration of collagen scaffolds used for local delivery of gentamicin in a rat mandible defect model. Bioengineering (Basel), 2022, 9(7): 275

[61] WangL, LiW, QuY, WangK, LvK, HeX, et al. . Preparation of super absorbent and highly active fish collagen sponge and its hemostatic effect in vivo and in vitro. Front Bioeng Biotechnol, 2022, 10: 862532

[62] HatzichristodoulouG. Evolution of the surgical sealing patch TachoSil® in Peyronie's disease reconstructive surgery: technique and contemporary literature review. World J Urol, 2020, 38(2): 315-321

[63] PetrieK, CoxCT, BeckerBC, MacKayBJ. Clinical applications of acellular dermal matrices: a review. Scars Burn Heal, 2022, 8: 20595131211038313

[64] JiangH, YuanMZ, WuZG, FangDB, GuoW, ShaoGF, et al. . Experts’ consensus on penile augmentation surgery. Chin J Androl, 2024, 38(04): 3-9

[65] YangM, ZhangY, FangC, SongL, WangY, LuL, et al. . Urine-microenvironment-initiated composite hydrogel patch reconfiguration propels scarless memory repair and reinvigoration of the urethra. Adv Mater, 2022, 34(14): e2109522

[66] DoerschKM, BarnettD, ChaseA, JohnstonD, GabrielsenJS. The contribution of the immune system to genitourinary fibrosis. Exp Biol Med (Maywood), 2022, 247(9): 765-778

[67] KirkJF, RitterG, FingerI, SankarD, ReddyJD, TaltonJD, et al. . Mechanical and biocompatible characterization of a cross-linked collagen-hyaluronic acid wound dressing. Biomatter, 2013, 3(4): e25633

[68] FuH, ZhangD, ZengJ, FuQ, ChenZ, SunX, et al. . Application of 3D-printed tissue-engineered skin substitute using innovative biomaterial loaded with human adipose-derived stem cells in wound healing. Int J Bioprint, 2023, 9(2): 674

[69] MangeraA, ChappleCR. Tissue engineering in urethral reconstruction–an update. Asian J Androl, 2013, 15(1): 89-92

[70] SievertKD, DaumL, MaurerS, ToomeyP, VaeglerM, AufderklammS, et al. . Urethroplasty performed with an autologous urothelium-vegetated collagen fleece to treat urethral stricture in the minipig model. World J Urol, 2020, 38(9): 2123-2131

[71] ChanEC, KuoSM, KongAM, MorrisonWA, DustingGJ, MitchellGM, et al. . Three dimensional collagen scaffold promotes intrinsic vascularisation for tissue engineering applications. PLoS ONE, 2016, 11(2): e0149799

[72] Puertas-BartoloméM, Włodarczyk-BiegunMK, Del CampoA, Vázquez-LasaB, SanRJ. 3D printing of a reactive hydrogel bio-ink using a static mixing tool. Polymers (Basel), 2020, 12(9): 1986

[73] Garcia-VillenF, GuembeA, JoséMR, ZúñigaT, Ruiz-AlonsoS, Saenz-Del-BurgoL, et al. . Characterization and assessment of new fibrillar collagen inks and bioinks for 3D printing and bioprinting. Int J Bioprint, 2023, 9(3): 712

[74] SalemM, KhadiviF, JavanbakhtP, MojaverrostamiS, AbbasiM, FeizollahiN, et al. . Advances of three-dimensional (3D) culture systems for in vitro spermatogenesis. Stem Cell Res Ther, 2023, 14(1): 262

[75] SabettaS, VecchiottiD, ClementiL, Di VitoNM, ZazzeroniF, AngelucciA. Comparative analysis of dasatinib effect between 2D and 3D tumor cell cultures. Pharmaceutics, 2023, 15(2): 372

[76] KimMJ, ChiBH, YooJJ, JuYM, WhangYM, ChangIH. Structure establishment of three-dimensional (3D) cell culture printing model for bladder cancer. PLoS ONE, 2019, 14(10): e0223689

[77] van GenderenAM, ValverdeMG, CapendalePE, KerstenMV, GarvíES, SchuurmansCCL, et al. . Co-axial printing of convoluted proximal tubule for kidney disease modeling. Biofabrication, 2022

[78] AddarioG, Fernández-PérezJ, FormicaC, KaryniotakisK, HerkensL, DjudjajS, et al. . 3D humanized bioprinted tubulointerstitium model to emulate renal fibrosis in vitro. Adv Healthc Mater, 2024

[79] StarborgT, KalsonNS, LuY, MironovA, CootesTF, HolmesDF, et al. . Using transmission electron microscopy and 3View to determine collagen fibril size and three-dimensional organization. Nat Protoc, 2013, 8(7): 1433-1448

[80] MiyataT, TairaT, NoishikiY. Collagen engineering for biomaterial use. Clin Mater, 1992, 9(3–4): 139-148

[81] ShouldersMD, RainesRT. Collagen structure and stability. Annu Rev Biochem, 2009, 78: 929-958

[82] TanrikuluIC, WestlerWM, EllisonAJ, MarkleyJL, RainesRT. templated collagen "double helices" maintain their structure. J Am Chem Soc, 2020, 142(3): 1137-1141

[83] BełdowskiP, PrzybyłekM, SionkowskaA, CysewskiP, GadomskaM, MusiałK, et al. . Effect of chitosan deacetylation on its affinity to type III collagen: a molecular dynamics study. Materials (Basel), 2022, 15(2): 463

[84] MajumdarS, WangX, SommerfeldSD, ChaeJJ, AthanasopoulouEN, ShoresLS, et al. . Cyclodextrin modulated type I collagen self-assembly to engineer biomimetic cornea implants. Adv Funct Mater, 2018, 28(41): 1804076

[85] LeiM, QuX, WanH, JinD, WangS, ZhaoZ, et al. . Electro-assembly of a dynamically adaptive molten fibril state for collagen. Sci Adv, 2022, 8(5): eabl7506

[86] KimBS, DasS, JangJ, ChoDW. Decellularized extracellular matrix-based bioinks for engineering tissue- and organ-specific microenvironments. Chem Rev, 2020, 120(19): 10608-10661

[87] ZhangX, ChenX, HongH, HuR, LiuJ, LiuC. Decellularized extracellular matrix scaffolds: recent trends and emerging strategies in tissue engineering. Bioact Mater, 2021, 10: 15-31

[88] YaoQ, ZhengYW, LanQH, KouL, XuHL, ZhaoYZ. Recent development and biomedical applications of decellularized extracellular matrix biomaterials. Mater Sci Eng C Mater Biol Appl, 2019, 104: 109942

[89] SartS, JeskeR, ChenX, MaT, LiY. Engineering stem cell-derived extracellular matrices: decellularization, characterization, and biological function. Tissue Eng Part B Rev, 2020, 26(5): 402-422

[90] RanaD, ZreiqatH, Benkirane-JesselN, RamakrishnaS, RamalingamM. Development of decellularized scaffolds for stem cell-driven tissue engineering. J Tissue Eng Regen Med, 2017, 11(4): 942-965

[91] YangY, LinQ, ZhouC, LiQ, LiZ, CaoZ, et al. . A testis-derived hydrogel as an efficient feeder-free culture platform to promote mouse spermatogonial stem cell proliferation and differentiation. Front Cell Dev Biol, 2020, 8: 250

[92] Moreno-ManzanoV, Mellado-LópezM, Morera-EsteveMJ, Alastrue-AgudoA, Bisbal-VelascoV, Forteza-VilaJ, et al. . Human adipose-derived mesenchymal stem cells accelerate decellularized neobladder regeneration. Regen Biomater, 2020, 7(2): 161-169

[93] SuJ, SatchellSC, ShahRN, WertheimJA. Kidney decellularized extracellular matrix hydrogels: rheological characterization and human glomerular endothelial cell response to encapsulation. J Biomed Mater Res A, 2018, 106(9): 2448-2462

[94] BashiriZ, AmiriI, GholipourmalekabadiM, FalakR, AsgariH, MakiCB, et al. . Artificial testis: a testicular tissue extracellular matrix as a potential bio-ink for 3D printing. Biomater Sci, 2021, 9(9): 3465-3484

[95] HadleyMA, ByersSW, Suárez-QuianCA, KleinmanHK, DymM. Extracellular matrix regulates sertoli cell differentiation, testicular cord formation, and germ cell development in vitro. J Cell Biol, 1985, 101(4): 1511-1522

[96] NaeemiS, EidiA, KhanbabaeeR, Sadri-ArdekaniH, KajbafzadehAM. Differentiation and proliferation of spermatogonial stem cells using a three-dimensional decellularized testicular scaffold: a new method to study the testicular microenvironment in vitro. Int Urol Nephrol, 2021, 53(8): 1543-1550

[97] VermeulenM, Del VentoF, KanbarM, Pyr Dit RuysS, VertommenD, PoelsJ, et al. . Generation of organized porcine testicular organoids in solubilized hydrogels from decellularized extracellular matrix. Int J Mol Sci, 2019, 20(21): 5476

[98] ZhangW, DuA, LiuS, LvM, ChenS. Research progress in decellularized extracellular matrix-derived hydrogels. Regen Ther, 2021, 18: 88-96

[99] ZhuJ, MarchantRE. Design properties of hydrogel tissue-engineering scaffolds. Expert Rev Med Devices, 2011, 8(5): 607-626

[100] StepanovskaJ, SupovaM, HanzalekK, BrozA, MatejkaR. Collagen bioinks for bioprinting: a systematic review of hydrogel properties, bioprinting parameters, protocols, and bioprinted structure characteristics. Biomedicines, 2021, 9(9): 1137

[101] WangX, RonsinO, GravezB, FarmanN, BaumbergerT, JaisserF, et al. . Nanostructured dense collagen-polyester composite hydrogels as amphiphilic platforms for drug delivery. Adv Sci (Weinh), 2021, 8(7): 2004213

[102] BuganimY, ItskovichE, HuYC, ChengAW, GanzK, SarkarS, et al. . Direct reprogramming of fibroblasts into embryonic Sertoli-like cells by defined factors. Cell Stem Cell, 2012, 11(3): 373-386

[103] GaoH, LiuC, WuB, CuiH, ZhaoY, DuanY, et al. . Effects of different biomaterials and cellular status on testicular cell self-organization. Adv Biosyst, 2020, 4(7): e1900292

[104] VolkovaN, YukhtaM, GoltsevA. Biopolymer gels as a basis of cryoprotective medium for testicular tissue of rats. Cell Tissue Bank, 2018, 19(4): 819-826

[105] AhmadS, AhmadM, ManzoorK, PurwarR, IkramS. A review on latest innovations in natural gums based hydrogels: preparations & applications. Int J Biol Macromol, 2019, 136: 870-890

[106] SharmaS, TiwariS. A review on biomacromolecular hydrogel classification and its applications. Int J Biol Macromol, 2020, 162: 737-747

[107] RidzewskiC, LiM, DongB, MagdanzV. Gelatin microcartridges for onboard activation and antioxidant protection of sperm. ACS Appl Bio Mater, 2020, 3(3): 1616-1627

[108] VardianiM, Ghaffari NovinM, KorujiM, NazarianH, GoossensE, AghaeiA, et al. . Gelatin electrospun mat as a potential co-culture system for in vitro production of sperm cells from embryonic stem cells. ACS Biomater Sci Eng, 2020, 6(10): 5823-5832

[109] JinY, SunP, WuT, WangJ, HuangX, ZuoS, et al. . Preparation of Gelatin/polycaprolactone electrospun fibers loaded with cis-platinum and their potential application for the treatment of prostate cancer. J Nanomater, 2021

[110] RazaF, SiyuL, ZafarH, KamalZ, ZhengB, SuJ, et al. . Recent advances in gelatin-based nanomedicine for targeted delivery of anti-cancer drugs. Curr Pharm Des, 2022, 28(5): 380-394

[111] FleckCA, SimmanR. Modern collagen wound dressings: function and purpose. J Am Col Certif Wound Spec, 2011, 2(3): 50-54

[112] TenorováK, MasteikováR, PavlokováS, KostelanskáK, BernatonienėJ, VetchýD. Formulation and evaluation of novel film wound dressing based on collagen/microfibrillated carboxymethylcellulose blend. Pharmaceutics, 2022, 14(4): 782

[113] MovaniyaPN, MakwanaTR, DesaiNN, MakwanaKG, PatelHB. Efficacy of collagen membrane graft in intraoral surgery - an evaluative study. Ann Maxillofac Surg, 2021, 11(1): 42-48

[114] WangY, WangG, HouX, ZhaoY, ChenB, DaiJ, et al. . Urethral tissue reconstruction using the acellular dermal matrix patch modified with collagen-binding VEGF in beagle urethral injury models. Biomed Res Int, 2021

[115] WuY, ChenS, LuoP, DengS, ShanZ, FangJ, et al. . Optimizing the bio-degradability and biocompatibility of a biogenic collagen membrane through cross-linking and zinc-doped hydroxyapatite. Acta Biomater, 2022, 143: 159-172

[116] RungS, ZhaoX, ChuC, YangR, QuY, ManY. Application of Epigallocatechin-3-gallate (EGCG) Modified 1-Ethyl-3-(3-dimethylaminopropylcarbodiimide hydrochloride/N-hydroxy-succinimide (EDC/NHS) cross-linked collagen membrane to promote macrophage adhesion. Materials (Basel), 2021, 14(16): 4660

[117] ZegersM, de BruijneMC, de KeizerB, MertenH, GroenewegenPP, van der WalG, et al. . The incidence, root-causes, and outcomes of adverse events in surgical units: implication for potential prevention strategies. Patient Saf Surg, 2011, 5: 13

[118] LewisKM, IkemeS, OlubunmiT, KuntzeCE. Clinical effectiveness and versatility of a sealing hemostatic patch (HEMOPATCH) in multiple surgical specialties. Expert Rev Med Devices, 2018, 15(5): 367-376

[119] EngvallE, RuoslahtiE, MillerEJ. Affinity of fibronectin to collagens of different genetic types and to fibrinogen. J Exp Med, 1978, 147(6): 1584-1595

[120] UlrichF, EttorreGM, WeltertL, OberhofferM, KreuwelH, De SantisR, et al. . Intra-operative use of hemopatch®: interim results of a nationwide european survey of surgeons. Surg Technol Int, 2016, 28: 19-28

[121] RickenbacherA, BreitensteinS, LesurtelM, FrillingA. Efficacy of TachoSil a fibrin-based haemostat in different fields of surgery–a systematic review. Expert Opin Biol Ther, 2009, 9(7): 897-907

[122] KrishnappaP, Fernandez-PascualE, CarballidoJ, MoncadaI, Lledo-GarciaE, Martinez-SalamancaJI. Surgical management of peyronie’s disease with co-existent erectile dysfunction. Sex Med, 2019, 7(4): 361-370

[123] SainiG, SegaranN, MayerJL, SainiA, AlbadawiH, OkluR. Applications of 3D bioprinting in tissue engineering and regenerative medicine. J Clin Med, 2021, 10(21): 4966

[124] MataiI, KaurG, SeyedsalehiA, McClintonA, LaurencinCT. Progress in 3D bioprinting technology for tissue/organ regenerative engineering. Biomaterials, 2020, 226: 119536

[125] DzoboK, MotaungKSCM, AdesidaA. Recent trends in decellularized extracellular matrix bioinks for 3D printing: an updated review. Int J Mol Sci, 2019, 20(18): 4628

[126] NohI, KimN, TranHN, LeeJ, LeeC. 3D printable hyaluronic acid-based hydrogel for its potential application as a bioink in tissue engineering. Biomater Res, 2019, 23: 3

[127] WangH, YuH, ZhouX, ZhangJ, ZhouH, HaoH, et al. . An overview of extracellular matrix-based bioinks for 3D bioprinting. Front Bioeng Biotechnol, 2022, 10: 905438

[128] Sobreiro-AlmeidaR, Gómez-FloritM, QuinteiraR, ReisRL, GomesME, NevesNM. Decellularized kidney extracellular matrix bioinks recapitulate renal 3D microenvironmentin vitro. Biofabrication, 2021

[129] AliM, PrAK, YooJJ, ZahranF, AtalaA, LeeSJ. A photo-crosslinkable kidney ECM-derived bioink accelerates renal tissue formation. Adv Healthc Mater, 2019, 8(7): e1800992

[130] MarquesCF, DiogoGS, PinaS, OliveiraJM, SilvaTH, ReisRL. Collagen-based bioinks for hard tissue engineering applications: a comprehensive review. J Mater Sci Mater Med, 2019, 30(3): 32

[131] RosetiL, CavalloC, DesandoG, ParisiV, PetrettaM, BartolottiI, et al. . Three-dimensional bioprinting of cartilage by the use of stem cells: a strategy to improve regeneration. Materials (Basel), 2018, 11(9): 1749

[132] ZhouF, HongY, LiangR, ZhangX, LiaoY, JiangD, et al. . Rapid printing of bio-inspired 3D tissue constructs for skin regeneration. Biomaterials, 2020, 258: 120287

[133] YangY, WangZ, XuY, XiaJ, XuZ, ZhuS, et al. . Preparation of chitosan/recombinant human collagen-based photo-responsive bioinks for 3D bioprinting. Gels, 2022, 8(5): 314

[134] JongprasitkulH, TurunenS, PariharVS, KellomäkiM. Sequential cross-linking of Gallic acid-functionalized GelMA-based bioinks with enhanced printability for extrusion-based 3D bioprinting. Biomacromol, 2023, 24(1): 502-514

[135] SaloniaA, BettocchiC, BoeriL, CapogrossoP, CarvalhoJ, CilesizNC, et al. . European association of urology guidelines on sexual and reproductive health-2021 update: male sexual dysfunction. Eur Urol, 2021, 80(3): 333-357

[136] RyuJK, ChoKS, KimSJ, OhKJ, KamSC, SeoKK, et al. . Korean society for Sexual medicine and andrology (KSSMA) guideline on erectile dysfunction. World J Mens Health, 2013, 31(2): 83-102

[137] FeldmanHA, GoldsteinI, HatzichristouDG, KraneRJ, McKinlayJB. Impotence and its medical and psychosocial correlates: results of the Massachusetts male aging study. J Urol, 1994, 151(1): 54-61

[138] HerutiR, ShochatT, Tekes-ManovaD, AshkenaziI, JustoD. Prevalence of erectile dysfunction among young adults: results of a large-scale survey. J Sex Med, 2004, 1(3): 284-291

[139] FengC, YangY, ChenL, GuoR, LiuH, LiC, et al. . Prevalence and characteristics of erectile dysfunction in obstructive sleep Apnea patients. Front Endocrinol (Lausanne), 2022, 13: 812974

[140] EardleyI, DonatucciC, CorbinJ, El-MeliegyA, HatzimouratidisK, McVaryK, et al. . Pharmacotherapy for erectile dysfunction. J Sex Me, 2010, 7: 524-540

[141] ConconiMT, NicoB, MangieriD, TommasiniM, di LiddoR, ParnigottoPP, et al. . Angiogenic response induced by acellular aortic matrix in vivo. Anat Rec A Discov Mol Cell Evol Biol, 2004, 281(2): 1303-1307

[142] YaguchiA, OshikawaM, WatanabeG, HiramatsuH, UchidaN, HaraC, et al. . Efficient protein incorporation and release by a jigsaw-shaped self-assembling peptide hydrogel for injured brain regeneration. Nat Commun, 2021, 12(1): 6623

[143] WangB, QinglaiT, YangQ, LiM, ZengS, YangX, et al. . Functional acellular matrix for tissue repair. Mater Today Bio., 2022, 18: 100530

[144] JemalA, SiegelR, XuJ, WardE. Cancer statistics. CA Cancer J Clin, 2010, 60(5): 277-300

[145] PotoskyAL, DavisWW, HoffmanRM, StanfordJL, StephensonRA, PensonDF, et al. . Five-year outcomes after prostatectomy or radiotherapy for prostate cancer: the prostate cancer outcomes study. J Natl Cancer Inst, 2004, 96(18): 1358-1367

[146] XieX, DuX, LiK, ChenY, GuanY, ZhaoX, et al. . Construction of engineered corpus cavernosum with primary mesenchymal stem cells in vitro. Sci Rep, 2017, 7(1): 18053

[147] KwonTG, YooJJ, AtalaA. Autologous penile corpora cavernosa replacement using tissue engineering techniques. J Urol, 2002, 168(4 Pt 2): 1754-1758

[148] ConnollySS, YooJJ, AbouhebaM, SokerS, McDougalWS, AtalaA. Cavernous nerve regeneration using acellular nerve grafts. World J Urol, 2008, 26(4): 333-339

[149] LinG, AlbersenM, HarrazAM, FandelTM, GarciaM, McGrathMH, et al. . Cavernous nerve repair with allogenic adipose matrix and autologous adipose-derived stem cells. Urology, 2011, 77(6): 1509.e1-8

[150] ChoMC, SongWH, PaickJS. Suppression of cavernosal fibrosis in a rat model. Sex Med Rev, 2018, 6(4): 572-582

[151] Qinyu-ZengS-H, Fengzhi-ChenL-W, Liren-ZhongJ-H, et al. . Administration of H2S improves erectile dysfunction by inhibiting phenotypic modulation of corpus cavernosum smooth muscle in bilateral cavernous nerve injury rats. Nitric Oxide, 2021

[152] YangS, ZhangY, LyuX, GuY, ZhangG, LiuP, et al. . The association between fgf21 and diabetic erectile dysfunction: evidence from clinical and animal studies. Front Endocrinol (Lausanne), 2022, 13: 874796

[153] SakakibaraY, TambaraK, SakaguchiG, LuF, YamamotoM, NishimuraK, et al. . Toward surgical angiogenesis using slow-released basic fibroblast growth factor. Eur J Cardiothorac Surg, 2003, 24(1): 105-111

[154] MaoAS, MooneyDJ. Regenerative medicine: current therapies and future directions. Proc Natl Acad Sci USA, 2015, 112(47): 14452-14459

[155] SuetomiT, HisasueS, SatoY, TabataY, AkazaH, TsukamotoT. Effect of basic fibroblast growth factor incorporating gelatin microspheres on erectile function in the diabetic rat. J Urol, 2005, 173(4): 1423-1428

[156] JungAR, ParkYH, JeonSH, KimGE, KimMY, SonJY, et al. . Therapeutic effect of controlled release of dual growth factor using heparin-pluronic hydrogel/gelatin-poly (ethylene glycol)-tyramine hydrogel system in a rat model of cavernous nerve injury. Tissue Eng Part A, 2018, 24(23–24): 1705-1714

[157] KimIG, PiaoS, LeeJY, HongSH, HwangTK, KimSW, et al. . Effect of an adipose-derived stem cell and nerve growth factor-incorporated hydrogel on recovery of erectile function in a rat model of cavernous nerve injury. Tissue Eng Part A, 2013, 19(1–2): 14-23

[158] GaoJ, PengD, ZhangX, HaoZ, ZhouJ, FanS, et al. . Prevalence and associated factors of premature ejaculation in the Anhui male population in China: evidence-based unified definition of lifelong and acquired premature ejaculation. Sex Med, 2017, 5(1): e37-43

[159] GulM, BocuK, SerefogluEC. Current and emerging treatment options for premature ejaculation. Nat Rev Urol, 2022, 19(11): 659-680

[160] McMahonCG, AlthofSE, WaldingerMD, PorstH, DeanJ, SharlipID, et al. . An evidence-based definition of lifelong premature ejaculation: report of the International society for sexual medicine (ISSM) ad hoc committee for the definition of premature ejaculation. J Sex Med, 2008, 5(7): 1590-1606

[161] ZuckerI, NackeeranS, KulkarniN, CartoC, MadhusoodananV, RamasamyR. Majority of men with premature ejaculation do not receive pharmacotherapy. Int J Impot, 2023, 35(6): 544-547

[162] CoskunerER, OzkanB. Premature ejaculation and endocrine disorders: a literature review. World J Mens Health, 2022, 40(1): 38-51

[163] WaldingerMD. Drug treatment options for premature ejaculation. Expert Opin Pharmacother, 2018, 19(10): 1077-1085

[164] AbourehabMA, AhmedOA, BalataGF, AlmalkiWH. Self-assembled biodegradable polymeric micelles to improve dapoxetine delivery across the blood-brain barrier. Int J Nanomedicine, 2018, 13: 3679-3687

[165] MantovaniF. Pharmacological/dynamic rehabilitative behavioural therapy for premature ejaculation: results of a pilot study. Arch Ital Urol Androl, 2017, 89(2): 148-150

[166] ZhangX, WuY, ZhangM, YinH, LiQ, BaiW, et al. . Acellular dermal matrix in premature ejaculation: a preliminary study. Medicine (Baltimore), 2018, 97(45): e13135

[167] WangH, ZhangH, BaiM, ZengA. Surgical treatment for primary premature ejaculation with inner condom technique. J Med Res, 2017, 46(9): 104-106

[168] MaZ, LiM, WangXS, WangQ, YuanMZ. Application of micronised acellular dermal matrix for primary premature ejaculation: a preliminary study. Andrologia, 2021, 53(4): e13994

[169] VealeD, MilesS, ReadJ, TrogliaA, WylieK, MuirG. Sexual functioning and behavior of men with body dysmorphic disorder concerning penis size compared with men anxious about penis size and with controls: a cohort study. Sex Med, 2015, 3(3): 147-155

[170] ChristensenJD, OtterbringT, LagerkvistCJ. Smaller prize, bigger size? Exploring the impact of money on men's self-reported markers of masculinity. Front Psychol, 2023, 14: 1105423

[171] VardiY. Is penile enlargement an ethical procedure for patients with a normal-sized penis?. Eur Urol, 2006, 49(4): 609-611

[172] XingMH, HouSW, RaheemOA. Aesthetic penile augmentation procedures: a comprehensive and current perspective. Curr Urol Rep, 2022, 23(12): 355-361

[173] CoskunerER, CanterHI. Desire for penile girth enhancement and the effects of the self-injection of hyaluronic Acid gel. J Cutan Aesthet Surg, 2012, 5(3): 198-200

[174] HehemannMC, ToweM, HuynhLM, El-KhatibFM, YafiFA. Penile girth enlargement strategies: what's the evidence?. Sex Med Rev, 2019, 7(3): 535-547

[175] FurrJ, HebertK, WisenbaughE, GelmanJ. Complications of genital enlargement surgery. J Sex Med, 2018, 15(12): 1811-1817

[176] ZhangCL, LiH, LiQ, BaiWJ, XuT, ZhangXW. Decision regret analysis among Chinese patients receiving penile girth enhancement with acellular dermal matrix. Beijing Da Xue Xue Bao Yi Xue Ban, 2020, 52(4): 678-683

[177] AleiG, LetiziaP, RicottilliF, SimoneP, AleiL, MassoniF, et al. . Original technique for penile girth augmentation through porcine dermal acellular grafts: results in a 69-patient series. J Sex Med, 2012, 9(7): 1945-1953

[178] ZhangH, JinC, ZhangP, WuY, ZhangM, BaiW, et al. . Human acellular dermal matrix augmentation phalloplasty surgery. Plast Surg (Oakv), 2020, 28(3): 161-166

[179] XuT, ZhangG, BaiW, LiQ, YangA, LinQ, et al. . Complications and management of penile girth enhancement with acellular dermal matrix. J Sex Med, 2019, 16(12): 2011-2017

[180] SiegelRL, MillerKD, WagleNS, JemalA. Cancer statistics, 2023. CA Cancer J Clin, 2023, 73(1): 17-48

[181] Nguyen-NielsenM, BorreM. Diagnostic and therapeutic strategies for prostate cancer. Semin Nucl Med, 2016, 46(6): 484-490

[182] AlivizatosG, SkolarikosA. Incontinence and erectile dysfunction following radical prostatectomy: a review. ScientificWorldJournal, 2005, 5: 747-758

[183] IyerR, NguyenT, PadanilamD, XuC, SahaD, NguyenKT, et al. . Glutathione-responsive biodegradable polyurethane nanoparticles for lung cancer treatment. J Control Release, 2020, 321: 363-371

[184] HeP, XuS, GuoZ, YuanP, LiuY, ChenY, et al. . Pharmacodynamics and pharmacokinetics of PLGA-based doxorubicin-loaded implants for tumor therapy. Drug Deliv, 2022, 29(1): 478-488

[185] FrankeK, BaurM, DaumL, VaeglerM, SievertKD, SchlosshauerB. Prostate carcinoma cell growth-inhibiting hydrogel supports axonal regeneration in vitro. Neurosci Lett, 2013, 541: 248-252

[186] TabataK, WatanabeM, NaruishiK, EdamuraK, SatohT, YangG, et al. . Therapeutic effects of gelatin matrix-embedded IL-12 gene-modified macrophages in a mouse model of residual prostate cancer. Prostate Cancer Prostatic Dis, 2009, 12(3): 301-309

[187] FitzgeraldKA, GuoJ, TierneyEG, CurtinCM, MalhotraM, DarcyR, et al. . The use of collagen-based scaffolds to simulate prostate cancer bone metastases with potential for evaluating delivery of nanoparticulate gene therapeutics. Biomaterials, 2015, 66: 53-66

[188] SongWH, LimYS, KimJE, KangHY, LeeC, RajbongshiL, et al. . A marine collagen-based 3D scaffold for in vitro modeling of human prostate cancer niche and anti-cancer therapeutic discovery. Mar Drugs, 2024, 22(7): 295

[189] LiEV, EsterquestR, PhamMN, PankenEJ, AmarasekeraC, SiebertA, et al. . Peyronie's disease: pharmacological treatments and limitations. Expert Rev Clin Pharmacol, 2021, 14(6): 703-713

[190] RandhawaK, ShuklaCJ. Non-invasive treatment in the management of Peyronie's disease. Ther Adv Urol, 2019, 11: 1756287218823671

[191] AllamehF, RazzaghiM, RayeganiSM, Fallah-KarkanM, RanjbarA, RahavianA, et al. . Laser therapy for peyronie's disease: a randomized control double-blind pilot study. J Lasers Med Sci, 2019, 10(Suppl 1): S37-42

[192] Bal-OzturkA, CecenB, Avci-AdaliM, TopkayaSN, AlarcinE, YasayanG, et al. . Tissue adhesives: from research to clinical translation. Nano Today, 2021, 36: 101049

[193] GocołR, BisJ, HudziakD, MorkiszŁ, DejaMA. Aortic root reconstruction with tachosil fibrin sealant patch in acute type a aortic dissection. Ann Thorac Cardiovasc Surg, 2021, 27(4): 267-272

[194] LahmeS, GötzT, BichlerKH. Collagen fleece for defect coverage following plaque excision in patients with Peyronie's disease. Eur Urol, 2002, 41(4): 401-405

[195] Fernández-PascualE, Gonzalez-GarcíaFJ, Rodríguez-MonsalveM, TuroJ, Martínez-BallesterosC, CarballidoJ, et al. . Surgical technique for complex cases of peyronie's disease with implantation of penile prosthesis, multiple corporeal incisions, and grafting with collagen fleece. J Sex Med, 2019, 16(2): 323-332

[196] SeyamRM, MandourahHM, KattanMS, Al-HussainT, AltaweelWM, KattanSA. A study of the histopathology of collagen fleece (TachoSil) patching of tunica albuginea in the rat penis and a literature review of penile graft materials in experimental animals. Transl Androl Urol, 2023, 12(8): 1238-1249

[197] HatzichristodoulouG, YangDY, RingJD, HebertKJ, ZiegelmanMJ, KöhlerTS. Multicenter experience using collagen fleece for plaque incision with grafting to correct residual curvature at the time of inflatable penile prosthesis placement in patients with Peyronie's disease. J Sex Med, 2020, 17(6): 1168-1174

[198] WuB, LuNX, XiaYK, GuAH, LuCC, WangW, et al. . A frequent Y chromosome b2/b3 subdeletion shows strong association with male infertility in Han-Chinese population. Hum Reprod, 2007, 22(4): 1107-1113

[199] CioppiF, RostaV, KrauszC. Genetics of azoospermia. Int J Mol Sci, 2021, 22(6): 3264

[200] KatzDJ, KolonTF, FeldmanDR, MulhallJP. Fertility preservation strategies for male patients with cancer. Nat Rev Urol, 2013, 10(8): 463-472

[201] RobinsonM, SparaneseS, WitherspoonL, FlanniganR. Human in vitro spermatogenesis as a regenerative therapy - where do we stand?. Nat Rev Urol, 2023, 20(8): 461-479

[202] LeeJH, GyeMC, ChoiKW, HongJY, LeeYB, ParkDW, et al. . In vitro differentiation of germ cells from nonobstructive azoospermic patients using three-dimensional culture in a collagen gel matrix. Fertil Steril, 2007, 87(4): 824-833

[203] StreetM, ThambyahA, DrayM, AmirapuS, TuariD, CallonKE, et al. . Augmentation with an ovine forestomach matrix scaffold improves histological outcomes of rotator cuff repair in a rat model. J Orthop Surg Res, 2015, 10: 165

[204] SiuMK, ChengCY. Extracellular matrix and its role in spermatogenesis. Adv Exp Med Biol, 2008, 636: 74-91

[205] HynesRO. The extracellular matrix: not just pretty fibrils. Science, 2009, 326(5957): 1216-1219

[206] BashiriZ, GholipourmalekabadiM, FalakR, AmiriI, AsgariH, ChauhanNPS, et al. . In vitro production of mouse morphological sperm in artificial testis bioengineered by 3D printing of extracellular matrix. Int J Biol Macromol, 2022, 217: 824-841

[207] NoghaniAE, AsadpourR, SaberivandA, MazaheriZ, HamidianG. Effect of NMDA receptor agonist and antagonist on spermatogonial stem cells proliferation in 2- and 3- dimensional culture systems. Mol Biol Rep, 2022, 49(3): 2197-2207

[208] WuJ, KangK, LiuS, MaY, YuM, ZhaoX. Recent progress of in vitro 3d culture of male germ stem cells. J Funct Biomater, 2023, 14(11): 543

[209] Ashouri MovassaghS, Ashouri MovassaghS, Banitalebi DehkordiM, PourmandG, GholamiK, TalebiA, et al. . Isolation, identification and differentiation of human spermatogonial cells on three-dimensional decellularized sheep testis. Acta Histochem, 2020, 122(8): 151623

[210] RicherG, HobbsRM, LovelandKL, GoossensE, BaertY. Long-term maintenance and meiotic entry of early germ cells in murine testicular organoids functionalized by 3d printed scaffolds and air-medium interface cultivation. Front Physiol, 2021, 12: 757565

[211] Majidi GharenazN, MovahedinM, MazaheriZ. Three-dimensional culture of mouse spermatogonial stem cells using a decellularised testicular scaffold. Cell J, 2020, 21(4): 410-418

[212] BaertY, StukenborgJB, LandrehM, De KockJ, JörnvallH, SöderO, et al. . Derivation and characterization of a cytocompatible scaffold from human testis. Hum Reprod, 2015, 30(2): 256-267

[213] Kargar-AbarghoueiE, VojdaniZ, HassanpourA, AlaeeS, Talaei-KhozaniT. Characterization, recellularization, and transplantation of rat decellularized testis scaffold with bone marrow-derived mesenchymal stem cells. Stem Cell Res Ther, 2018, 9(1): 324

[214] HeY, WangC, WangC, XiaoY, LinW. An overview on collagen and Gelatin-based cryogels: fabrication, classification, properties and biomedical applications. Polymers (Basel), 2021, 13(14): 2299

[215] PlanteG, ManjunathP. Epididymal binder of SPerm genes and proteins: what do we know a decade later?. Andrology, 2015, 3(5): 817-824

[216] ManjunathP, LefebvreJ, JoisPS, FanJ, WrightMW. New nomenclature for mammalian BSP genes. Biol Reprod, 2009, 80(3): 394-397

[217] Shimokawa KiK, KatayamaM, MatsudaY, TakahashiH, HaraI, SatoH, et al. . Matrix metalloproteinase (MMP)-2 and MMP-9 activities in human seminal plasma. Mol Hum Reprod, 2002, 8(1): 32-36

[218] LeeR, ParkHJ, LeeWY, ChoiY, SongH. Nanoscale level gelatin-based scaffolds enhance colony formation of porcine testicular germ cells. Theriogenology, 2023, 202: 125-135

[219] VardianiM, GholipourmalekabadiM, Ghaffari NovinM, KorujiM, Ghasemi HamidabadiH, SalimiM, et al. . Three-dimensional electrospun gelatin scaffold coseeded with embryonic stem cells and sertoli cells: a promising substrate for in vitro coculture system. J Cell Biochem, 2019, 120(8): 12508-12518

[220] BashiriZ, ZahiriM, AllahyariH, EsmaeilzadeB. Proliferation of human spermatogonial stem cells on optimized PCL/Gelatin nanofibrous scaffolds. Andrologia, 2022, 54(5): e14380

[221] XuX, FengQ, MaX, DengY, ZhangK, OoiHS, et al. . Dynamic gelatin-based hydrogels promote the proliferation and self-renewal of embryonic stem cells in long-term 3D culture. Biomaterials, 2022, 289: 121802

[222] Abu ElhijaM, LunenfeldE, SchlattS, HuleihelM. Differentiation of murine male germ cells to spermatozoa in a soft agar culture system. Asian J Androl, 2012, 14(2): 285-293

[223] KhadiviF, KorujiM, AkbariM, JabariA, TalebiA, Ashouri MovassaghS, et al. . Application of platelet-rich plasma (PRP) improves self-renewal of human spermatogonial stem cells in two-dimensional and three-dimensional culture systems. Acta Histochem, 2020, 122(8): 151627

[224] KhajaviN, AkbariM, AbdolsamadiHR, AbolhassaniF, DehpourAR, KorujiM, et al. . Role of somatic testicular cells during mouse spermatogenesis in three-dimensional collagen gel culture system. Cell J, 2014, 16(1): 79-90

[225] LeeJH, KimHJ, KimH, LeeSJ, GyeMC. In vitro spermatogenesis by three-dimensional culture of rat testicular cells in collagen gel matrix. Biomaterials, 2006, 27(14): 2845-2853

[226] DolkH, LoaneM, GarneE. The prevalence of congenital anomalies in Europe. Adv Exp Med Biol, 2010, 686: 349-364

[227] Arenas da SilvaLF, MicolL, TiemessenD, van KuppeveltTH, FreyP, OosterwijkE, et al. . Is there a need for smooth muscle cell transplantation in urethral reconstruction. Tissue Eng Part A, 2014, 20(9–10): 1542-1549

[228] De FilippoRE, KornitzerBS, YooJJ, AtalaA. Penile urethra replacement with autologous cell-seeded tubularized collagen matrices. J Tissue Eng Regen Med, 2015, 9(3): 257-264

[229] OrabiH, AbouShwarebT, ZhangY, YooJJ, AtalaA. Cell-seeded tubularized scaffolds for reconstruction of long urethral defects: a preclinical study. Eur Urol, 2013, 63(3): 531-538

[230] PinnagodaK, LarssonHM, VythilingamG, VardarE, EngelhardtEM, ThambidoraiRC, et al. . Engineered acellular collagen scaffold for endogenous cell guidance, a novel approach in urethral regeneration. Acta Biomater, 2016, 43: 208-217

[231] WuS, YeC, YangH, ChenB, NieH, LiS. Application of allogeneic human acellular dermal matrix reduces the incidence of fistula in hypospadias repair. Front Pediatr, 2022, 10: 774973

[232] LinD, WangG, SongH, QuY, LiuP, LiangH, et al. . Use of acellular dermal matrix for urethroplasty coverage in proximal hypospadias repair: a pilot study. Adv Ther, 2020, 37(4): 1425-1435

[233] TangX, ZhangX, WuY, YinH, DuY, ZhangX, et al. . The clinical effects of utilizing allogeneic acellular dermal matrix in the surgical therapy of anterior urethral stricture. Urol Int, 2020, 104(11–12): 933-938

[234] LinJ, HaoJR, JinJ, DengSM, HuJ, NaYQ. Homologous dermal acellular matrix graft for urethral reconstruction in man (report of 16 cases). Zhonghua Yi Xue Za Zhi, 2005, 5(15): 1057-1059

[235] El KassabyA, AbouShwarebT, AtalaA. Randomized comparative study between buccal mucosal and acellular bladder matrix grafts in complex anterior urethral strictures. J urol, 2008, 179(4): 1432-1436

[236] MandalTK, DhanukaS, ChoudhuryS, MukhopadhyayBC, KayalA, MajhiTK, et al. . Tissue engineered indigenous pericardial patch urethroplasty: a promising solution to a nagging problem. Asian J Urol., 2020, 7(1): 56-60

[237] XuK, HanY, HuangY, WeiP, YinJ, JiangJ. The application of 3D bioprinting in urological diseases. Mater Today Bio, 2022, 16: 100388

[238] WangB, LiZL, ZhangYL, WenY, GaoYM, LiuBC. Hypoxia and chronic kidney disease. EBioMedicine, 2022, 77: 103942

[239] JansenK, SchuurmansCCL, JansenJ, MasereeuwR, VermondenT. Hydrogel-based cell therapies for kidney regeneration: current trends in biofabrication and in vivo repair. Curr Pharm Des, 2017, 23(26): 3845-3857

[240] GBD Chronic Kidney Disease Collaboration. Global, regional, and national burden of chronic kidney disease, 1990–2017: a systematic analysis for the global burden of disease study 2017. Lancet, 2020, 395(10225): 709-733

[241] ZhaoW, HuC, XuT. In vivo bioprinting: Broadening the therapeutic horizon for tissue injuries. Bioact Mater., 2023, 25: 201-222

[242] HomanKA, KoleskyDB, Skylar-ScottMA, HerrmannJ, ObuobiH, MoisanA, et al. . Bioprinting of 3D convoluted renal proximal tubules on perfusable chips. Sci Rep, 2016, 6: 34845

[243] LinNYC, HomanKA, RobinsonSS, KoleskyDB, DuarteN, MoisanA, et al. . Renal reabsorption in 3D vascularized proximal tubule models. Proc Natl Acad Sci U S A, 2019, 116(12): 5399-5404

[244] SinghNK, HanW, NamSA, KimJW, KimJY, KimYK, et al. . Three-dimensional cell-printing of advanced renal tubular tissue analogue. Biomaterials, 2020, 232: 119734

Funding

National Natural Science Foundation of China(82305302)

Chian Postodoctoral Science Foundation(2022M722295)

Key Research Institute of Humanities and Social Sciences in Sichuan Province(2022YFS0380)

RIGHTS & PERMISSIONS

The Author(s)