INTRODUCTION
COMMONLY USED XENOPUS SPECIES IN LABORATORY
DIFFERENT TYPES OF XENOPUS CELL-FREE EXTRACTS
1 Xenopus cell-free extracts. Progesterone (PG) induces prophase I-arrested Xenopus oocyte to mature. At GVBD, white spots appear at the animal hemisphere of the maturing oocytes, and the mature oocytes arrest at metaphase II due to CSF, which is fertilizable. After fertilization, the CSF is released, and the mature eggs enter interphase and start synchronous embryonic cell division until blastula stage VIII when the synchrony is lost. In the laboratory, the fertilization is simulated by the addition of calcium ionophore A23187. Prophase I-arrested oocytes are used to prepare prophase I oocyte extracts. MI-arrested (by cold treatment) oocytes are used to prepare MI oocyte extracts. MII-arrested eggs are used to prepare CSF extracts, and interphase-mitosis extracts are prepared from the freshly prepared CSF extracts with the addition of calcium. Activated eggs are used to prepare cycling extracts. Early-stage embryos are used to prepare embryo extracts, which can be arrested in mitosis with the addition of cell cycle regulators |
Oocyte extract
Prophase I oocyte extract
MI oocyte extract
Egg extracts
CSF extracts
Fresh CSF extracts
2 Xenopus egg/embryo extracts. A MII-arrested Xenopus eggs are crushed by high-speed centrifugation to make CSF extract. B The CSF extract is added with calcium to release CSF arrest to allow the extract to enter interphase. With the addition of freshly prepared CSF extract, the system is driven back to and arrest at mitosis, and the extract is sometimes called interphase-mitosis extract. C MII-arrested Xenopus eggs are released to interphase with the addition of calcium ionophore A23187. Forty-five minutes after the calcium ionophore addition, the CSF-released eggs are used to prepare the cycling extract. D Mature Xenopus eggs are fertilized by sperm slurry prepared from Xenopus males and early embryonic development starts. These early Xenopus embryos at different development stages, e.g. Stage IV or Stage VIII, are used to prepare embryo extract. These extracts are added with cell cycle regulators such as Δ90-cyclin B and UbcH10-C114S to arrest the extracts at mitosis |
Frozen CSF extracsts
Interphase-mitosis extract
Cycling extract
Embryo extracts
INVESTIGATION OF SPINDLE ASSEMBLY IN XENOPUS CELL-FREE EXTRACTS
Spindle assembly in Xenopus cell-free extracts
3 Two pathways to spindle assembly in vitro. A In Xenopus CSF extract, each sperm nucleus contains one centrosome and directs the assembly of the one-half spindle. Two half spindles then fuse and form a bipolar spindle. B In extracts that go through interphase, the centrosome and DNA of the sperm nuclei will replicate, and each sperm nucleus is capable of the assembly of a bipolar spindle in this case |