Biophysics Reports >> 2023, Vol.9 >> Issue (4) : 188 - 194 https://doi.org/10.52601/bpr.2023.230011
 

Reconstitution of membrane contact by unilamellar vesicles

Shulin Li, Min Zhang, Liang Ge
1 State Key Laboratory of Membrane Biology, Beijing 100101, China
2 Tsinghua-Peking Center for Life Sciences, Beijing 100084, China
3 School of Life Sciences, Tsinghua University, Beijing 100084, China
4 School of Pharmaceutical Sciences, Tsinghua University, Beijing 100084, China
 
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Abstract

Eukaryotic cells compartmentalize diverse biochemical functions within organelles defined by intracellular membranes. Recent focus has intensified on studying the interactions among organelles and the role of membrane contacts in maintaining cellular balance. While analyzing these contacts mainly involves fluorescence and electron microscopy, as well as biochemical cell fractionation, understanding their mechanisms and responses to genetic and environmental changes remains challenging. Here we describe an approach employing in vitro reconstitution of membrane contacts using unilamellar vesicles. This technique offers insights into contact mechanisms when combined with established methods like fluorescence imaging and mass spectrometry, potentially deepening our understanding of membrane contacts and organelle networks.

Keywords : Membrane contacts, Organelle interaction network, Giant unilamellar vesicle (GUV), Liposome, ER-Golgi intermediate compartment (ERGIC), ER-exit sites (ERES), COPII, TMED9, SEC12, Autophagy, Autophagosome
Corresponding Author(s): Liang Ge
Issue Date: 31 Aug 2023
 
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