Streamlined process for effective and strand-selective mitochondrial base editing using mitoBEs
Xiaoxue Zhang, Zongyi Yi, Wei Tang, Wensheng Wei
Streamlined process for effective and strand-selective mitochondrial base editing using mitoBEs
Mitochondrial base editing tools hold great promise for the investigation and treatment of mitochondrial diseases. Mitochondrial DNA base editors (mitoBEs) integrate a programmable transcription-activator-like effector (TALE) protein with single-stranded DNA deaminase (TadA8e-V106W, APOBEC1, etc.) and nickase (MutH, Nt.BspD6I(C), etc.) to achieve heightened precision and efficiency in mitochondrial base editing. This innovative mitochondrial base editing tool exhibits a number of advantages, including strand-selectivity for editing, high efficiency, and the capacity to perform diverse types of base editing on the mitochondrial genome by employing various deaminases. In this context, we provide a detailed experimental protocol for mitoBEs to assist others in achieving proficient mitochondrial base editing.
Mitochondrial DNA base editors (mitoBEs) / Transcription-activator-like effector (TALE) / Deaminase / Nickase
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