PDF(3032 KB)
Non-invasive micro-test technology and applications
Kai Sun, Yunqi Liu, Yanshu Pan, Dongwei Di, Jianfang Li, Feiyun Xu, Li Li, Yoshiharu Mimata, Yingying Chen, Lixia Xie, Siqi Wang, Wenqian Qi, Yan Tang, Huachun Sheng, Bing Wang, Ruixue Sun, Dingquan Tan, Daohong Fu, Ye Yin, Ao Xue, Yichao Shi, Wenjing Shao, Lei Gong, Zhijian Jiang, Wei Zhang, Qiangsheng Wu, Yaosheng Wang, Minglin Lang, Wenxiu Ye, Weifeng Xu, Shuhe Wei, Weiming Shi, Yue Jeff Xu
Biophysics Reports ›› 2025, Vol. 11 ›› Issue (2) : 96-111.
PDF(3032 KB)
PDF(3032 KB)
Non-invasive micro-test technology and applications
Non-invasive micro-test technology (NMT) reveals dynamic ionic/molecular concentration gradients by measuring fluxes of ions and small molecules in liquid media in 1D, 2D or 3D fashions with sensitivity up to pico- (10−12) or femto- (10−15) moles per cm2 per second. NMT has been applied to study metabolism, signal transduction, genes and/or proteins physiological functions related to transmembrane ionic/molecular activities with live samples under normal conditions or stress. Data on ion and/or molecule homeostasis (IMH) by NMT in biomedical sciences, plant and crop sciences, environmental sciences, marine and space biology as well as traditional Chinese medicine are reviewed.
Many thanks to Prof. Tingyun Kuang (Institute of Botany, Chinese Academy of Sciences), Prof Lionel Jaffe (Marine Biological Laboratory, The University of Chicago), Prof. Fuyu Yang (Institute of Biophysics, Chinese Academy of Sciences), Prof. Kechun Lin (Peking University), Prof. Weiping Zhao (Capital Normal University), Prof. Zesheng Qiu (Capital Normal University), Prof. Weihua Wu (China Agricultural University), Prof. Kang Chong (Institute of Botany, the Chinese Academy of Sciences), Prof. Weicai Yang (Institute of Genetics and Developmental Biology, Chinese Academy of Sciences), Prof. Yan Guo (China Agricultural University), Prof. Tao Xu (Institute of Biophysics, Chinese Academy of Sciences), Prof. Shaoliang Chen (Beijing Forestry University), Prof. Zhibin Luo (Institute of Forestry, Chinese Academy of Forestry Sciences), Prof. Liwei Wang (Jinan University), Prof. Lixin Chen (Jinan University), Prof. Xingjie Liang (National Center for Nanoscience and Technology), Prof. Jian Sun (Jiangsu Normal University), Prof. Jinxia Zhu (Capital Medical University), Prof. Jinshun Qi (Shanxi Medical University), Prof. Marshall Porterfield (Purdue University), Prof. Sergey Shabala (The University of Western Australia), Prof. Lana Shabala (University of Tasmania). We thank Prof. Li Zhang (Peking Union Medical College Hospital), Prof. Ning Yu (Chinese People's Liberation Army (PLA) General Hospital), Prof. Jingqian Feng (Medical School, Quzhou College of Technology), Prof. Lihong Wang (Scholl of Environment and Civil Engineering, Jiangnan University), Dr. Heping Wan (School of Life Sciences, Jianghan University), Ms. Wen Zhang (School of Life Sciences, Shandong University) and Mr. Jianfei Song (College of Horticulture Science and Engineering, Shandong Agricultural University) for critical reading of the manuscript.
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