An efficient two-step subcellular fractionation method for the enrichment of insulin granules from INS-1 cells

Keywords

Insulin / Immature insulin secretary granules (iISGs) / Mature insulin secretary granules (mISGs) / Subcellular fractionation / Density gradient

References

Ahras M, Otto GP, Tooze SA (2006) Synaptotagmin IV is necessary for the maturation of secretory granules in PC12 cells. J Cell Biol 173:241-251
Bock JB, Lin RC, Scheller RH (1996) A new syntaxin family member implicated in targeting of intracellular transport vesicles. J Biol Chem 271:17961-17965
Borgonovo B, Ouwendijk J, Solimena M (2006) Biogenesis of secretory granules. Curr Opin Cell Biol 18:365-370
Brunner Y, Coute Y,Iezzi M, Foti M, Fukuda M, Hochstrasser DF, Wollheim CB, Sanchez JC (2007) Proteomics analysis of insulin secretory granules. Mol Cell Proteomics 6:1007-1017
Colomer V, Kicska GA, Rindler MJ (1996) Secretory granule content proteins and the luminal domains of granule mem-brane proteins aggregate in vitro at mildly acidic pH. J Biol Chem 271:48-55
D'Amico F, Skarmoutsou E, Imbesi RM, Sanfilippo S (2001) Early events of secretory granule formation in the rat parotid acinar cell under the influence of isoproterenol. An ultrastructural and lectin cytochemical study. Eur J Histochem 45:169-175
Davidson HW (2004) (Pro)insulin processing-a historical per-spective. Cell Biochem Biophys 35(1):143-157
Dittie AS, Tooze SA (1995) Characterization of the endopeptidase PC2 activity towards secretogranin II in stably transfected PC12 cells. Biochem J 310(Pt 3):777-787
Docherty K, Hutton JC (1983) Carboxypeptidase activity in the insulin secretory granule. FEBS Lett 162:137-141
Hendershot LM, Valentine VA, Lee AS, Morris SW, Shapiro DN (1994) Localization of the gene encoding human Bip/Grp78 the endoplasmic-reticulum cognate of the Hsp70 family, to chromosome-9q34. Genomics 20:281-284
Huber LA, Pfaller K, Vietor I (2003) Organelle proteomics-implications for subcellular fractionation in proteomics. Circ Res 92:962-968
Hutton JC, Hansen F, Peshavaria M (1985) Beta-granins: 21 kDa co-secreted peptides of the insulin granule closely related to adrenal medullary chromogranin A. FEBS Lett 188:336-340
Iezzi M, Escher G, Meda只Charollais A, Baldini G, DarchenE Wollheim CB, Regazzi R (1999) Subcellular distribution and function of Rab3A, B, C, and D isoforms in insulin-secreting cells. Mol Endocrinol 73:202-272
Kim T, Gondre-Lewis MC, Arnaoutova I, Loh YP (2006) Dense-core secretory granule biogenesis. Physiology 21:124-133
Klumperman J, Kuliawat R, Griffith JM, Geuze HJ, Arvan P (1998) Mannose 6-phosphate receptors are sorted from immature secretory granules via adaptor protein AP-1, clathrin, and syntaxin 6-positive vesicles. J Cell Biol 141:359-371
Konrad RJ, Young RA, Record RD, Smith RM, Butkerait P, Manning D, Jarett L, Wolf BA (1995) The heterotrimeric G-Protein G(I) is localized to the insulin secretory granules of beta-cells and is involved in insulin exocytosis. J Biol Chem 270:12869-12876
Mast S, Peng L, Jordan TW, Flint H, Phillips L, Donaldson L, Strabala TJ,wagner A (2010) Proteomic analysis of mem-brane preparations from developing Pinus radiata compres-sion wood. Tree Physiol 30:1456-1468
McCrossan M, Windsor M, Ponnambalam S, Armstrong J, Wileman T (2001) The trans Golgi network is lost from cells infected with African swine fever virus. J Virol 75:11755-11765
Mundy DI,Wamen G (1992) Mitosis and inhibition of intracellular transport stimulate palmitoylation of a 62-kD protein. J Cell Biol 116:135-146
Nakagawa M, Miranda AF (1987) A monoclonal antibody against cytochrome c oxidase distinguishes cardiac and skeletal muscle mitochondria. Exp Cell Res 168:44-52
Orci L, Ravazzola M, Amherdt M, Madsen 0, Perrelet A, Vassalli JD, Anderson RGW (1986) Conversion of proinsulin to insulin occurs coordinately with acidification of maturing secretory vesicles. J Cell Biol 103:2273-2281
Rhodes C (2000) Processing of the insulin molecule. In: Diabetes Mellitus: a fundamental and clinical text, p 27-50
Schvartz D, Brunner Y, Coute Y, Foti M, Wollheim CB, Sanchez JC (2012) Improved characterization of the insulin secretory granule proteomes. J Proteomics 75:4620-4631
Smeekens SP, Montag AG, Thomas G, Albigesrizo C, Carroll R, Benig M, Phillips LA, Martin S, Ohagi S, Gardner P et al (1992) Proinsulin processing by the subtilisin-related proprotein convertases furin, Pct, and Pc3. Proc Natl Acad Sci USA 89:8822-8826
Steiner DF, Park SY, Stoy J, Philipson LH, Bell GI (2009) A brief perspective on insulin production. Diabetes Obes Metab 11:189-196
Tooze SA, Stinchcombe JC (1992) Biogenesis of secretory granules. Semin Cell Riol 3:357-3hh
Tooze SA, Flatmark T, Tooze J, Huttner WB (1991) Characteriza-tion of the immature secretory granule, an intermediate in granule biogenesis. J Cell Biol 115:1491-1503
Wasmeier C, Hutton JC (1996) Molecular cloning of phogrin, a protein-tyrosine phosphatase homologue localized to insulin secretory granule membranes. J Biol Chem 271:18161-78770
Weir GC, Laybutt DR, Kaneto H, Bonner-Weir S, Sharma A (2001) Beta-cell adaptation and decompensation during the progression of diabetes. Diabetes 50(Suppl 1):5154-5159
Wendler F, Page L, Urbe S, Tooze SA (2001) Homotypic fusion of immature secretory granules during maturation requires syntaxin 6. Mol Biol Cell 12:1699-1709