Functional immunopeptides: advancing prevention and therapeutic strategies against animal diseases

Abstract

Peptide-based therapies have emerged as groundbreaking advancements in both therapeutic and preventive strategies against infectious diseases. These approaches utilize innovative functional immunopeptides—such as antigenic peptides, antimicrobial, immune modulation, and delivery peptides derived from pathogens or hosts—to target specific immune mechanisms. In addition to their simplicity of use, peptide-based approaches provide several advantages. These include improved specificity and immunogenicity by targeting specific antigenic peptides and enhanced delivery of particular proteins or vaccines to targeted immune cells, which increases the efficiency of antigen presentation and provides a self-adjuvant effect and therapeutic properties. The most recent developments in peptide-based systems to increase vaccine efficacy and therapeutic interventions for animal diseases are investigated in this review. It encompasses fundamental ideas, immunomodulating functions, and peptide production techniques. Additionally, the improvements and synergistic advantages attained by combining these functional immunopeptides with vaccines or using them as stand-alone therapeutic agents are emphasized. This review demonstrates how peptide-based treatments in veterinary medicine enhance immune responses and inhibit or eliminate pathogens.

Keywords

Immune regulation / Peptide-based strategies / Vaccine optimization / Medical and Health Sciences / Immunology

References

[1] AdhikariS, AlahmadiTI, GongZ, KarlssonAJ. Expression of Cell-Penetrating Peptides Fused to Protein Cargo. Journal of Molecular Microbiology and Biotechnology, 2018, 284159-168.

[2] AggarwalS, SomaniVK, GuptaS, GargR, BhatnagarR. Development of a novel multiepitope chimeric vaccine against anthrax. Medical Microbiology and Immunology, 2019, 2082185-195.

[3] AhmedS, RahmanMN, HasanM, HasanMA, MiaMM. Immunogenic multiepitope-based vaccine development to combat cyclosporiasis of immunocompromised patients applying computational biology method. Experimental Parasitology, 2023, 248. 108497

[4] AlahyaribeikS, NazarpourM. Peptide recovery from chicken feather keratin and their anti-biofilm properties against methicillin-resistant Staphylococcus aureus (MRSA). World Journal of Microbiology & Biotechnology, 2024, 404123.

[5] AlavarseAC, FrachiniECG, da SilvaRLCG, LimaVH, ShavandiA, PetriDFS. Crosslinkers for polysaccharides and proteins: Synthesis conditions, mechanisms, and crosslinking efficiency, a review. International Journal of Biological Macromolecules, 2022, 202: 558-596.

[6] Al-HalifaS, ZottigX, BabychM, Côté-CyrM, BourgaultS, ArchambaultD. Harnessing the Activation of Toll-Like Receptor 2/6 by Self-Assembled Cross-β Fibrils to Design Adjuvanted Nanovaccines. Nanomaterials (Basel, Switzerland), 2020, 10101981.

[7] AllahyariM, GolkarM, Fard-EsfahaniP, Dimier-PoissonI, MévélecM-N. Codelivery of PLGA nanoparticles loaded with rSAG1 antigen and TLR ligands: An efficient vaccine against chronic toxoplasmosis. Microbial Pathogenesis, 2022, 162. 105312

[8] AloisioA, NisticòN, MimmiS, MaisanoD, VecchioE, FiumeG, IaccinoE, QuintoI. Phage-Displayed Peptides for Targeting Tyrosine Kinase Membrane Receptors in Cancer Therapy. Viruses, 2021, 134649.

[9] ApteSH, StephensonRJ, SimerskaP, GrovesPL, AljohaniS, EskandariS, TothI, DoolanDL. Systematic evaluation of self-adjuvanting lipopeptide nanovaccine platforms for the induction of potent CD8(+) T-cell responses. Nanomedicine (London, England), 2016, 112137-152.

[10] Aziz, S., Almajhdi, F. N., Waqas, M., Ullah, I., Salim, M. A., Khan, N. A., & Ali, A. (2022). Contriving multiepitope vaccine ensemble for monkeypox disease using an immunoinformatics approach. Frontiers in Immunology, 13. https://doi.org/10.3389/fimmu.2022.1004804

[11] AzmiF, Ahmad FuaadAAH, SkwarczynskiM, TothI. Recent progress in adjuvant discovery for peptide-based subunit vaccines. Human Vaccines & Immunotherapeutics, 2014, 103778-796.

[12] BacklundCM, HoldenRL, MoynihanKD, GarafolaD, FarquharC, MehtaNK, MaiorinoL, PhamS, IorgulescuJB, ReardonDA, et al. . Cell-penetrating peptides enhance peptide vaccine accumulation and persistence in lymph nodes to drive immunogenicity. Proceedings of the National Academy of Sciences, 2022, 11932. e2204078119

[13] BaoR, MaZ, StanfordK, McAllisterTA, NiuYD. Antimicrobial Activities of α-Helix and β-Sheet Peptides against the Major Bovine Respiratory Disease Agent, Mannheimia hemolytica. International Journal of Molecular Sciences, 2024, 2584164.

[14] BhardwajG, MulliganVK, BahlCD, GilmoreJM, HarveyPJ, ChenevalO, BuchkoGW, PulavartiSVSRK, KaasQ, EletskyA, et al. . Accurate de novo design of hyperstable constrained peptides. Nature, 2016, 5387625329-335.

[15] BlancoE, CubillosC, MorenoN, BárcenaJ, De La TorreBG, AndreuD, SobrinoF. B Epitope Multiplicity and B/T Epitope Orientation Influence Immunogenicity of Foot-and-Mouth Disease Peptide Vaccines. Clinical and Developmental Immunology, 2013, 2013: 1-9.

[16] CaiM, ZhuF, WuH, ShenP. A new recombinant hybrid polypeptide and its immunologic adjuvant activity for inactivated infectious bursal disease vaccine. Biotechnology Letters, 2014, 3671431-1437.

[17] CalisJJA, MaybenoM, GreenbaumJA, WeiskopfD, De SilvaAD, SetteA, KeşmirC, PetersB. Properties of MHC class I presented peptides that enhance immunogenicity. PLoS Computational Biology, 2013, 910. e1003266

[18] ChangC-Y, ChengI-C, ChangY-C, TsaiP-S, LaiS-Y, HuangY-L, JengC-R, PangVF, ChangH-W. Identification of Neutralizing Monoclonal Antibodies Targeting Novel Conformational Epitopes of the Porcine Epidemic Diarrhea Virus Spike Protein. Scientific Reports, 2019, 912529.

[19] ChenX, YangJ, WangL, LiuB. Personalized neoantigen vaccination with synthetic long peptides: Recent advances and future perspectives. Theranostics, 2020, 10136011-6023.

[20] Cifuentes-RiusA, DesaiA, YuenD, JohnstonAPR, VoelckerNH. Inducing immune tolerance with dendritic cell-targeting nanomedicines. Nature Nanotechnology, 2021, 16137-46.

[21] CornejoS, BarberC, ThoresenM, LawrenceM, SeoKS, WoolumsA. Synthetic antimicrobial peptides Bac-5, BMAP-28, and Syn-1 can inhibit bovine respiratory disease pathogens in vitro. Frontiers in Veterinary Science, 2024, 11: 1430919.

[22] DassanayakeRP, AtkinsonBM, MullisAS, FalkenbergSM, NicholsonEM, CasasE, NarasimhanB, BearsonSMD. Bovine NK-lysin peptides exert potent antimicrobial activity against multidrug-resistant Salmonella outbreak isolates. Scientific Reports, 2021, 11: 19276.

[23] DengZ, ZhangS, SunM, YangH, LuY, WangM, FangW, ShiF, HeF. Nanobodies against porcine CD163 as PRRSV broad inhibitor. International Journal of Biological Macromolecules, 2023, 253Pt 7. 127493

[24] DeyJ, MahapatraSR, SinghPK, PrabhuswamimathSC, MisraN, SuarM. Designing of multiepitope peptide vaccine against Acinetobacter baumannii through combined immunoinformatics and protein interaction-based approaches. Immunologic Research, 2023, 714639-662.

[25] DiamondG, BeckloffN, WeinbergA, KisichKO. The roles of antimicrobial peptides in innate host defense. Current Pharmaceutical Design, 2009, 15212377-2392.

[26] Dietze-SchwonbergK, GreweB, BroschS, KuharevJ, van ZandbergenG, RammenseeH-G, TenzerS, von StebutE. In silico prediction of Leishmania major-specific CD8+ epitopes. Experimental Dermatology, 2017, 269838-840.

[27] Duarte-MataDI, Salinas-CarmonaMC. Antimicrobial peptides´ immune modulation role in intracellular bacterial infection. Frontiers in Immunology, 2023, 14: 1119574.

[28] FengL, ChenL, YunJ, CaoX. Expression of recombinant classical swine fever virus E2 glycoprotein by endogenous Txnip promoter in stable transgenic CHO cells. Engineering in Life Sciences, 2020, 208320-330.

[29] FogaçaAC, AlmeidaIC, EberlinMN, TanakaAS, BuletP, DaffreS. Ixodidin, a novel antimicrobial peptide from the hemocytes of the cattle tick Boophilus microplus with inhibitory activity against serine proteinases. Peptides, 2006, 274667-674.

[30] FornerM, Cañas-ArranzR, DefausS, de LeónP, Rodríguez-PulidoM, GangesL, BlancoE, SobrinoF, AndreuD. Peptide-Based Vaccines: Foot-and-Mouth Disease Virus, a Paradigm in Animal Health. Vaccines, 2021, 95477.

[31] ForoutanM, GhaffarifarF, SharifiZ, DalimiA. Vaccination with a novel multiepitope ROP8 DNA vaccine against acute Toxoplasma gondii infection induces strong B and T-cell responses in mice. Comparative Immunology, Microbiology and Infectious Diseases, 2020, 69. 101413

[32] GanzT. The role of antimicrobial peptides in innate immunity. Integrative and Comparative Biology, 2003, 432300-304.

[33] Gessner, I., & Neundorf, I. (2020). Nanoparticles modified with cell-penetrating peptides: conjugation mechanisms, physicochemical properties, and application in cancer diagnosis and therapy. International Journal of Molecular Sciences, 21(7), Article 7. https://doi.org/10.3390/ijms21072536

[34] González-Stegmaier, R., Peña, A., Villarroel-Espíndola, F., Aguila, P., Oliver, C., MacLeod-Carey, D., Rozas-Serri, M., Enriquez, R., & Figueroa, J. (2021). Full recombinant flagellin B from Vibrio anguillarum (rFLA) and its recombinant D1 domain (rND1) promote a pro-inflammatory state and improve vaccination against P. salmonis in Atlantic salmon (S. salar). Developmental and Comparative Immunology, 117, 103988. https://doi.org/10.1016/j.dci.2020.103988

[35] GorkaM, CherepanovDA, SemenovAY, GolbeckJH. Control of electron transfer by protein dynamics in photosynthetic reaction centers. Critical Reviews in Biochemistry and Molecular Biology, 2020, 555425-468.

[36] HabermannSM, MurphyKP. Energetics of hydrogen bonding in proteins: A model compound study. Protein Science, 1996, 571229-1239.

[37] HamleyIW. Peptides for Vaccine Development. ACS Applied Bio Materials, 2022, 53905-944.

[38] HanK, ZhaoD, LiuY, HuangX, YangJ, LiuQ, AnF, LiY. Design and evaluation of a polytope construct with multiple B and T epitopes against Tembusu virus infection in ducks. Research in Veterinary Science, 2016, 104: 174-180.

[39] HanagataN. CpG oligodeoxynucleotide nanomedicines for the prophylaxis or treatment of cancers, infectious diseases, and allergies. International Journal of Nanomedicine, 2017, 12: 515-531.

[40] HaoS, ShiW, ChenL, KongT, WangB, ChenS, GuoX. CATH-2-derived antimicrobial peptide inhibits multidrug-resistant Escherichia coli infection in chickens. Frontiers in Cellular and Infection Microbiology, 2024, 14: 1390934.

[41] HongY, TruongAD, LeeJ, LeeK, KimG-B, HeoK-N, LillehojHS, HongYH. Identification of duck liver-expressed antimicrobial peptide 2 and characterization of its bactericidal activity. Asian-Australasian Journal of Animal Sciences, 2019, 3271052-1061.

[42] HouJ, LiuZ, CaoS, WangH, JiangC, HussainMA, PangS. Broad-Spectrum Antimicrobial Activity and Low Cytotoxicity against Human Cells of a Peptide Derived from Bovine αS1-Casein. Molecules (Basel, Switzerland), 2018, 2351220.

[43] HuG, MiaoY, LuoX, ChuW, FuY. Identification of a novel cell-penetrating peptide derived from the capsid protein of chicken anemia virus and its application in gene delivery. Applied Microbiology and Biotechnology, 2020, 1042410503-10513.

[44] HuanY, KongQ, MouH, YiH. Antimicrobial Peptides: Classification, Design, Application and Research Progress in Multiple Fields. Frontiers in Microbiology, 2020, 11. 582779

[45] J, L., J, H., Dr, P., Jjh, C., A, G., Bw, S., R, M., E, A., & S, A. (2024). Discovery of a new class of cell-penetrating peptides by novel phage display platform. Scientific Reports, 14(1). https://doi.org/10.1038/s41598-024-64405-w

[46] JeanC, BoulianneM, BrittenM, RobitailleG. Antimicrobial activity of buttermilk and lactoferrin peptide extracts on poultry pathogens. The Journal of Dairy Research, 2016, 834497-504.

[47] JeffersonRE, OggierA, FüglistalerA, CamvielN, HijaziM, VillarrealAR, ArberC, BarthP. Computational design of dynamic receptor-peptide signaling complexes applied to chemotaxis. Nature Communications, 2023, 1412875.

[48] JiangY, HuJ, GuoY, YangW, YeL, ShiC, LiuY, YangG, WangC. Construction and immunological evaluation of recombinant Lactobacillus plantarum expressing HN of Newcastle disease virus and DC- targeting peptide fusion protein. Journal of Biotechnology, 2015, 216: 82-89.

[49] JiangN, ShrotriyaP, DassanayakeRP. NK-lysin antimicrobial peptide-functionalized nanoporous alumina membranes as biosensors for label-free bacterial endotoxin detection. Biochemical and Biophysical Research Communications, 2022, 636Pt 218-23.

[50] JinY-B, YangW-T, ShiC-W, FengB, HuangK-Y, ZhaoG-X, LiQ-Y, XieJ, HuangH-B, JiangY-L, et al. . Immune responses induced by recombinant Lactobacillus plantarum expressing the spike protein derived from transmissible gastroenteritis virus in piglets. Applied Microbiology and Biotechnology, 2018, 102198403-8417.

[51] JoshiVG, DigheVD, ThakuriaD, MalikYS, KumarS. Multiple antigenic peptide (MAP): A synthetic peptide dendrimer for diagnostic, antiviral and vaccine strategies for emerging and re-emerging viral diseases. Indian Journal of Virology: An Official Organ of Indian Virological Society, 2013, 243312-320.

[52] KabaSA, KarchCP, SethL, FerlezKMB, StormeCK, PesaventoDM, LaughlinPY, Bergmann-LeitnerES, BurkhardP, LanarDE. Self-assembling protein nanoparticles with built-in flagellin domains increases protective efficacy of a Plasmodium falciparum based vaccine. Vaccine, 2018, 366906-914.

[53] KajiwaraN, NomuraN, UkajiM, YamamotoN, KoharaM, YasuiF, SakodaY, KidaH, ShibasakiF. Cell-penetrating peptide-mediated cell entry of H5N1 highly pathogenic avian influenza virus. Scientific Reports, 2020, 10118008.

[54] KangQ, SunZ, ZouZ, WangM, LiQ, HuX, LiN. Cell-penetrating peptide-driven Cre recombination in porcine primary cells and generation of marker-free pigs. PLoS ONE, 2018, 131. e0190690

[55] KarPP, AravetiPB, KuriakoseA, SrivastavaA. Design of a multiepitope protein as a subunit vaccine against lumpy skin disease using an immunoinformatics approach. Scientific Reports, 2022, 12119411.

[56] Karch, C. P., Li, J., Kulangara, C., Paulillo, S. M., Raman, S. K., Emadi, S., Tan, A., Helal, Z. H., Fan, Q., Khan, M. I., & Burkhard, P. (2017). Vaccination with self-adjuvanted protein nanoparticles provides protection against lethal influenza challenge. Nanomedicine: Nanotechnology, Biology, and Medicine, 13(1), 241–251. https://doi.org/10.1016/j.nano.2016.08.030

[57] KarunnanithyV, Abdul RahmanNHB, AbdullahNAH, FauziMB, LokanathanY, Min HweiAN, MaarofM. Effectiveness of Lyoprotectants in Protein Stabilization During Lyophilization. Pharmaceutics, 2024, 16101346.

[58] KathayatD, ClossG, HelmyYA, LokeshD, RanjitS, RajashekaraG. Peptides Affecting the Outer Membrane Lipid Asymmetry System (MlaA-OmpC/F) Reduce Avian Pathogenic Escherichia coli (APEC) Colonization in Chickens. Applied and Environmental Microbiology, 2021, 8717. e0056721

[59] KayeshMEH, KoharaM, Tsukiyama-KoharaK. TLR agonists as vaccine adjuvants in the prevention of viral infections: An overview. Frontiers in Microbiology, 2023, 14: 1249718.

[60] KazemiM, AghamaaliMR, MadaniR, EmamiT, GolchinfarF. Evaluating the Immunogenicity of recombinant VP1 protein from the foot-and-mouth disease virus encapsulated in nanoliposome in guinea pig animal model. Veterinary Immunology and Immunopathology, 2022, 253. 110497

[61] KimJ-S, JoengJ-H, KimY. Design, Characterization, and Antimicrobial Activity of a Novel Antimicrobial Peptide Derived from Bovine Lactophoricin. Journal of Microbiology and Biotechnology, 2017, 274759-767.

[62] KopeikinPM, ZharkovaMS, KolobovAA, SmirnovaMP, SukharevaMS, UmnyakovaES, KokryakovVN, OrlovDS, MilmanBL, BalandinSV, et al. . Caprine Bactenecins as Promising Tools for Developing New Antimicrobial and Antitumor Drugs. Frontiers in Cellular and Infection Microbiology, 2020, 10. 552905

[63] KumarR, AliSA, SinghSK, BhushanV, MathurM, JamwalS, MohantyAK, KaushikJK, KumarS. Antimicrobial Peptides in Farm Animals: An Updated Review on Its Diversity, Function, Modes of Action and Therapeutic Prospects. Veterinary Sciences, 2020, 74206.

[64] LeNMT, ThereforeK-K, ChunJ, KimD-H. Expression of virus-like particles (VLPs) of foot-and-mouth disease virus (FMDV) using Saccharomyces cerevisiae. Applied Microbiology and Biotechnology, 2024, 108181.

[65] LeiJ, SunL, HuangS, ZhuC, LiP, HeJ, MackeyV, CoyDH, HeQ. The antimicrobial peptides and their potential clinical applications. American Journal of Translational Research, 2019, 1173919-3931

[66] LiW, JoshiMD, SinghaniaS, RamseyKH, MurthyAKPeptide Vaccine: Progress and Challenges. Vaccines, 2014, 23515-536.

[67] LiJ, HelalZH, KarchCP, MishraN, GirshickT, GarmendiaA, BurkhardP, KhanMI. A self-adjuvanted nanoparticle based vaccine against infectious bronchitis virus. PLoS ONE, 2018, 139. e0203771

[68] LiZ, HuY, YangY, LuZ, WangY. Antimicrobial resistance in livestock: Antimicrobial peptides provide a new solution for a growing challenge. Animal Frontiers: The Review Magazine of Animal Agriculture, 2018, 8221-29.

[69] LiD, WuJ, ChenJ, ZhangD, ZhangY, QiaoX, YuX, ZhengQ, HouJ. Optimized expression of classical swine fever virus E2 protein via combined strategy in Pichia pastoris. Protein Expression and Purification, 2020, 167. 105527

[70] LiH, NiuJ, WangX, NiuM, LiaoC. The Contribution of Antimicrobial Peptides to Immune Cell Function: A Review of Recent Advances. Pharmaceutics, 2023, 1592278.

[71] LiQ, WubshetAK, WangY, HeathL, ZhangJ. B and T-Cell Epitopes of the Incursionary Foot-and-Mouth Disease Virus Serotype SAT2 for Vaccine Development. Viruses, 2023, 153797.

[72] LiJ, WangH, WangL, YuD, ZhangX. Stabilization effects of saccharides in protein formulations: A review of sucrose, trehalose, cyclodextrins and dextrans. European Journal of Pharmaceutical Sciences, 2024, 192. 106625

[73] LiangW, ZhouG, LiuW, YangB, LiC, WangH, YangD, MaW, YuL. Identification of a conserved linear neutralizing epitope recognized by monoclonal antibody 9A9 against serotype A foot-and-mouth disease virus. Archives of Virology, 2016, 161102705-2716.

[74] Ligtenberg, A. J. M., Bikker, F. J., & Bolscher, J. G. M. (2021). LFchimera: A synthetic mimic of the two antimicrobial domains of bovine lactoferrin. Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire, 99(1), 128–137. https://doi.org/10.1139/bcb-2020-0285

[75] LindeY, OvadiaO, SafraiE, XiangZ, PortilloFP, ShalevDE, Haskell-LuevanoC, HoffmanA, GilonC. Structure-activity relationship and metabolic stability studies of backbone cyclization and N-methylation of melanocortin peptides. Peptide Science, 2008, 905671-682.

[76] LiuS, TuC, WangC, YuX, WuJ, GuoS, ShaoM, GongQ, ZhuQ, KongX. The protective immune response induced by B-cell epitope of classical swine fever virus glycoprotein E2. Journal of Virological Methods, 2006, 1341–2125-129.

[77] LiuS, YuX, WangC, WuJ, KongX, TuC. Quadruple antigenic epitope peptide producing immune protection against classical swine fever virus. Vaccine, 2006, 2449–507175-7180.

[78] LiuW, YangB, WangM, WangH, YangD, MaW, ZhouG, YuL. Identification of a conformational neutralizing epitope on the VP1 protein of type A foot-and-mouth disease virus. Research in Veterinary Science, 2017, 115: 374-381.

[79] LiuX, LvJ, FangY, ZhouP, LuY, PanL, ZhangZ, MaJ, ZhangY, WangY. Expression and Immunogenicity of Two Recombinant Fusion Proteins Comprising Foot-and-Mouth Disease Virus Structural Protein VP1 and DC-SIGN-Binding Glycoproteins. BioMed Research International, 2017, 2017: 7658970.

[80] LiuA, KrushnamurthyPH, SubramanyaKS, MitchellDA, MahantaN. Enzymatic thioamidation of peptide backbones. Methods in Enzymology, 2021, 656: 459-494.

[81] LiuM, FangX, YangY, WangC. Peptide-Enabled Targeted Delivery Systems for Therapeutic Applications. Frontiers in Bioengineering and Biotechnology, 2021, 9. 701504

[82] LiuR, LiuZ, PengH, LvY, FengY, KangJ, LuN, MaR, HouS, SunW, et al. . Bomidin: An Optimized Antimicrobial Peptide With Broad Antiviral Activity Against Enveloped Viruses. Frontiers in Immunology, 2022, 13. 851642

[83] LiuZ-H, DengZ-F, LuY, FangW-H, HeF. A modular and self-adjuvanted multivalent vaccine platform based on porcine circovirus virus-like nanoparticles. Journal of Nanobiotechnology, 2022, 201493.

[84] LiuX, WangX, ShiX, WangS, ShaoK. The immune enhancing effect of antimicrobial peptide LLv on broilers chickens. Poultry Science, 2024, 1032. 103235

[85] LongQ, WeiM, WangY, PangF. Design of a multiepitope vaccine against goatpox virus using an immunoinformatics approach. Frontiers in Cellular and Infection Microbiology, 2023, 13: 1309096.

[86] LuchnerM, ReinkeS, MilicicA. TLR Agonists as Vaccine Adjuvants Targeting Cancer and Infectious Diseases. Pharmaceutics, 2021, 132142.

[87] LundO, KarosieneE, LundegaardC, LarsenMV, NielsenMBioinformatics Identification of Antigenic Peptide: Predicting the Specificity of Major MHC Class I and II Pathway Players., 2013, 960: 247-260.

[88] MaS, QiaoX, XuY, WangL, ZhouH, JiangY, CuiW, HuangX, WangX, TangL, et al. . Screening and Identification of a Chicken Dendritic Cell Binding Peptide by Using a Phage Display Library. Frontiers in Immunology, 2019, 10: 1853.

[89] MaX, ChenZ, LongM. Mechanisms of Action, Biological Characteristics, and Future Prospects: A Review of Antimicrobial Peptides (A Review). Applied Biochemistry and Microbiology, 2024.

[90] MadaniF, LindbergS, LangelÜ, FutakiS, GräslundA. Mechanisms of Cellular Uptake of Cell-Penetrating Peptides. Journal of Biophysics, 2011, 20111. 414729

[91] MahmoudMM, Al-HejinAM, AbujamelTS, GhetasAM, YacoubHA. Chicken β-defensin-1 peptide as a candidate anticoccidial agent in broiler chickens. Animal Biotechnology, 2023, 3473108-3125.

[92] MalonisRJ, LaiJR, VergnolleO. Peptide-Based Vaccines: Current Progress and Future Challenges. Chemical Reviews, 2020, 12063210-3229.

[93] MamaboloMV, TheronJ, MareeF, CramptonM. Production of foot-and-mouth disease virus SAT2 VP1 protein. AMB Express, 2020, 1012.

[94] Marques-BastosSLS, CoelhoMLV, de Sousa SantosIN, MorenoDSA, BarriasES, de MendonçaJFM, MendonçaLC, LangeCC, de Paiva BritoMAV, do Carmo de Freire Bastos, M. . Effects of the natural antimicrobial peptide aureocin A53 on cells of Staphylococcus aureus and Streptococcus agalactiae involved in bovine mastitis in the excised teat model. World Journal of Microbiology & Biotechnology, 2022, 3915.

[95] Matsuda, T., Misato, K., Tamiya, S., Akeda, Y., Nakase, I., Kuroda, E., Takahama, S., Nonaka, M., Yamamoto, T., Fukuda, M. N., et al. (2022). Efficient antigen delivery by dendritic cell-targeting peptide via nucleolin confers superior vaccine effects in mice. iScience, 25(11). https://doi.org/10.1016/j.isci.2022.105324

[96] Melgoza-GonzálezEA, Bustamante-CórdovaL, HernándezJ. Recent advances in antigen targeting to antigen-presenting cells in veterinary medicine. Frontiers in Immunology, 2023, 14: 1080238.

[97] Michel-TodóL, BigeyP, RechePA, PinazoM-J, GascónJ, Alonso-PadillaJ. Design of an Epitope-Based Vaccine Ensemble for Animal Trypanosomiasis by Computational Methods. Vaccines, 2020, 81130.

[98] MingfuN, QiangG, YangL, YingH, ChengshuiL, CuiliQ. The antimicrobial peptide MetchnikowinII enhances Ptfa antigen immune responses against avian Pasteurella multocida in chickens. The Journal of Veterinary Medical Science, 2023, 859964-971.

[99] MoorthyBS, IyerLK, ToppEM. Characterizing Protein Structure, Dynamics and Conformation in Lyophilized Solids. Current Pharmaceutical Design, 2015, 21405845-5853.

[100] Miller, S.M., R. J. Simon, S. Ng, R. N. Zuckermann, J. M. Kerr, & W. H. Moos. 1995. Comparison of the proteolytic susceptibilities of homologous L-amino acid, D-amino acid, and N-substituted glycine peptide and peptoid oligomers. Drug Development Research 35:20–32. https://doi.org/10.1002/ddr.430350105.

[101] Mustafa, G., Mahrosh, H. S., Salman, M., Ali, M., Arif, R., Ahmed, S., & Ebaid, H. (2023). In silico analysis of honey bee peptides as potential inhibitors of capripoxvirus DNA-directed RNA polymerase. Animals: An Open Access Journal from MDPI, 13(14), 2281. https://doi.org/10.3390/ani13142281

[102] NiuY, LiuY, YangL, QuH, ZhaoJ, HuR, LiJ, LiuW. Immunogenicity of multiepitope-based vaccine candidates administered with the adjuvant Gp96 against rabies. Virologica Sinica, 2016, 312168-175.

[103] OuyangJ, ShengY, WangW. Recent Advances of Studies on Cell-Penetrating Peptides Based on Molecular Dynamics Simulations. Cells, 2022, 11244016.

[104] OuyangR, OngenaeV, MuokA, ClaessenD, BriegelA. Phage fibers and spikes: A nanoscale Swiss army knife for host infection. Current Opinion in Microbiology, 2024, 77. 102429

[105] ParkH, ParkJ-H, KimMS, ChoK, ShinJ-M. In Silico Screening and Optimization of Cell-Penetrating Peptides Using Deep Learning Methods. Biomolecules, 2023, 133522.

[106] ParkerKC, ShieldsM, DiBrinoM, BrooksA, ColiganJE. Peptide binding to MHC class I molecules: Implications for antigenic peptide prediction. Immunologic Research, 1995, 14134-57.

[107] ParvizpourS, PourseifMM, RazmaraJ, RafiMA, OmidiY. Epitope-based vaccine design: A comprehensive overview of bioinformatics approaches. Drug Discovery Today, 2020, 2561034-1042.

[108] PastorY, ReynardO, IampietroM, SurenaudM, PicardF, El JahraniN, LefebvreC, HammoudiA, DupatyL, BrisebardÉ, et al. . A vaccine targeting antigen-presenting cells through CD40 induces protective immunity against Nipah disease. Cell Reports. Medicine, 2024, 53. 101467

[109] PimchanT, TianF, ThumanuK, RodtongS, YongsawatdigulJ. Isolation, identification, and mode of action of antibacterial peptides derived from egg yolk hydrolysate. Poultry Science, 2023, 1027. 102695

[110] Popitool, K., Wataradee, S., Wichai, T., Noitang, S., Ajariyakhajorn, K., Charoenrat, T., Boonyaratanakornkit, V., & Sooksai, S. (2022). Potential of Pm11 antimicrobial peptide against bovine mastitis pathogens. American Journal of Veterinary Research, 84(1), ajvr.22.06.0096. https://doi.org/10.2460/ajvr.22.06.0096

[111] QianF, GuoA, LiM, LiuW, PanZ, JiangL, WuX, XuH. Salmonella flagellin is a potent carrier-adjuvant for peptide conjugate to induce peptide-specific antibody response in mice. Vaccine, 2015, 33172038-2044.

[112] Rádis-BaptistaG. Cell-Penetrating Peptides Derived from Animal Venoms and Toxins. Toxins, 2021, 132147.

[113] RajasekaranG, KumarSD, YangS, ShinSY. The design of a cell-selective fowlicidin-1-derived peptide with both antimicrobial and anti-inflammatory activities. European Journal of Medicinal Chemistry, 2019, 182. 111623

[114] RaniNA, RobinTB, PromeAA, AhmedN, MoinAT, PatilRB, SikderMNA, BappyMNI, AfrinD, HossainFMA, et al. . Development of multi epitope subunit vaccines against emerging carp viruses Cyprinid herpesvirus 1 and 3 using immunoinformatics approach. Scientific Reports, 2024, 14111783.

[115] RaoJP, AgrawalP, MohammadR, RaoSK, ReddyGR, DechammaHJ, SuryanarayanaS, V. V. . Expression of VP1 protein of serotype A and O of foot-and-mouth disease virus in transgenic sunnhemp plants and its immunogenicity for guinea pigs. Acta Virologica, 2012, 560291-99.

[116] ReedSG, OrrMT, FoxCB. Key roles of adjuvants in modern vaccines. Nature Medicine, 2013, 19121597-1608.

[117] Rezaei, M., Rabbani-khorasgani, M., Zarkesh-Esfahani, S. H., Emamzadeh, R., & Abtahi, H. (n.d.). Prediction of the Omp16 Epitopes for the Development of an Epitope-based Vaccine Against Brucellosis. Infectious Disorders - Drug Targets, 19(1), 36–45. https://doi.org/10.2174/1871526518666180709121653

[118] RobledoSM, Pérez-SilanesS, Fernández-RubioC, PovedaA, MonzoteL, GonzálezVM, Alonso-ColladoP, CarriónJ. Neglected Zoonotic Diseases: Advances in the Development of Cell-Penetrating and Antimicrobial Peptides against Leishmaniosis and Chagas Disease. Pathogens (Basel, Switzerland), 2023, 127939.

[119] RodriguesG, Souza SantosL, FrancoOL. Antimicrobial Peptides Controlling Resistant Bacteria in Animal Production. Frontiers in Microbiology, 2022, 13. 874153

[120] Roque-BordaCA, PereiraLP, GuastalliEAL, SoaresNM, Mac-LeanPAB, SalgadoDD, MeneguinAB, ChorilliM, VicenteEF. HPMCP-Coated Microcapsules Containing the Ctx(Ile21)-Ha Antimicrobial Peptide Reduce the Mortality Rate Caused by Resistant Salmonella Enteritidis in Laying Hens. Antibiotics (Basel, Switzerland), 2021, 106616.

[121] Roque-BordaCA, SilvaHRL, Crusca JuniorE, SerafimJA, MeneguinAB, ChorilliM, MacedoWC, TeixeiraSR, GuastalliEAL, SoaresNM, et al. . Alginate-based microparticles coated with HPMCP/AS cellulose-derivatives enable the Ctx (Ile21)-Ha antimicrobial peptide application as a feed additive. International Journal of Biological Macromolecules, 2021, 183: 1236-1247.

[122] Rossino, G., Marchese, E., Galli, G., Verde, F., Finizio, M., Serra, M., Linciano, P., & Collina, S. (2023). Peptides as therapeutic agents: Challenges and opportunities in the green transition Era. Molecules, 28(20), Article 20. https://doi.org/10.3390/molecules28207165

[123] RuY, HaoR, WuC, LiY, LuB, LiuH, TianH, LiD, ShiZ, LuoJ, et al. . Identification of Potential Novel B-Cell Epitopes of Capsid Protein VP2 in Senecavirus A. Microbiology Spectrum, 2023, 114. e0447222

[124] Saeed, S. I., Mergani, A., Aklilu, E., & Kamaruzzaman, N. F. (2022). Antimicrobial peptides: Bringing solution to the rising threats of antimicrobial resistance in livestock. Frontiers in Veterinary Science, 9. https://doi.org/10.3389/fvets.2022.851052

[125] SengkhuiS, KlubthaweeN, AunpadR. A novel designed membrane-active peptide for the control of foodborne Salmonella enterica serovar Typhimurium. Scientific Reports, 2023, 1313507.

[126] Shen, S., Ren, F., He, J., Wang, J., Sun, Y., & Hu, J. (2023). Recombinant antimicrobial peptide OaBac5 mini alleviates inflammation in pullorum disease chicks by modulating TLR4/MyD88/NF-κB pathway. Animals: An Open Access Journal from MDPI, 13(9), 1515. https://doi.org/10.3390/ani13091515

[127] ShiS-H, YangW-T, YangG-L, ZhangX-K, LiuY-Y, ZhangL-J, YeL-P, HuJ-T, XingX, QiC, et al. . Lactobacillus plantarum vaccine vector expressing hemagglutinin provides protection against H9N2 challenge infection. Virus Research, 2016, 211: 46-57.

[128] SilvaFD, RossiDCP, MartinezLR, FrasesS, FonsecaFL, CamposCBL, RodriguesML, NosanchukJD, DaffreS. Effects of microplusin, a copper-chelating antimicrobial peptide, against Cryptococcus neoformans. FEMS Microbiology Letters, 2011, 324164-72.

[129] SitinjakMC, ChenJ-K, WangC-Y. Characterization of novel cell-penetrating peptides derived from the capsid protein of beak and feather disease virus. Virus Research, 2023, 330. 199109

[130] St PaulM, BrisbinJT, BarjestehN, VillaneuevaAI, ParviziP, ReadLR, NagyE, SharifS. Avian influenza virus vaccines containing Toll-like receptors 2 and 5 ligand adjuvants promote protective immune responses in chickens. Viral Immunology, 2014, 274160-166.

[131] StephensAJ, Burgess-BrownNA, JiangS. Beyond Just Peptide Antigens: The Complex World of Peptide-Based Cancer Vaccines. Frontiers in Immunology, 2021, 12. 696791

[132] SunY, QianJ, XuX, TangY, XuW, YangW, JiangY, YangG, DingZ, CongY, et al. . Dendritic cell-targeted recombinantLactobacilli induce DC activation and elicit specific immune responses against G57 genotype of avian H9N2 influenza virus infection. Veterinary Microbiology, 2018, 223: 9-20.

[133] SunY, LiuK, YunT, NiZ, ZhuY, ChenL, BaoH, YeW, HuaJ, HuoS, et al. . High expression of the classical swine fever virus (CSFV) envelope protein E2 by a single amino acid mutation and its embedded in the pseudorabies virus (PRV) vector for immunization. Virus Research, 2023, 331. 199111

[134] SunY, GaoY, SuT, ZhangL, ZhouH, ZhangJ, SunH, BaiJ, JiangP. Nanoparticle Vaccine Triggers Interferon-Gamma Production and Confers Protective Immunity against Porcine Reproductive and Respiratory Syndrome Virus. ACS Nano, 2025, 191852-870.

[135] TaiH-M, YouM-F, LinC-H, TsaiT-Y, PanC-Y, ChenJ-Y. Scale-up production of and dietary supplementation with the recombinant antimicrobial peptide tilapia piscidin 4 to improve growth performance in Gallus gallus domesticus. PLoS ONE, 2021, 166. e0253661

[136] Takagi, S., Hayashi, S., Takahashi, K., Isogai, H., Bai, L., Yoneyama, H., Ando, T., Ito, K., & Isogai, E. (2012). Antimicrobial activity of a bovine myeloid antimicrobial peptide (BMAP-28) against methicillin-susceptible and methicillin-resistant Staphylococcus aureus. Animal Science Journal = Nihon Chikusan Gakkaiho, 83(6), 482–486. https://doi.org/10.1111/j.1740-0929.2011.00979.x

[137] TalapkoJ, MeštrovićT, JuzbašićM, TomasM, ErićS, Horvat AleksijevićL, BekićS, SchwarzD, MatićS, NeubergM, et al. . Antimicrobial Peptides-Mechanisms of Action, Antimicrobial Effects and Clinical Applications. Antibiotics (Basel, Switzerland), 2022, 11101417.

[138] TanL, ZhangY, LiuF, YuanY, ZhanY, SunY, QiuX, MengC, SongC, DingC. Infectious bronchitis virus poly-epitope-based vaccine protects chickens from acute infection. Vaccine, 2016, 34445209-5216.

[139] TanhaieanA, AzghandiM, RazmyarJ, MohammadiE, SekhavatiMH. Recombinant production of a chimeric antimicrobial peptide in E. coli and assessment of its activity against some avian clinically isolated pathogens. Microbial Pathogenesis, 2018, 122: 73-78.

[140] TesfagaberW, LanD, WangW, ZhaoR, YinL, YangM, ZhuY, SunE, LiuR, LinW, et al. . Identification of two novel B-cell epitopes on E184L protein of African swine fever virus using monoclonal antibodies. Virus Research, 2024, 346. 199412

[141] Thueng-InK, TheerawatanasirikulS, MeechanP, LekcharoensukP, ChaicumpaW. Cell-penetrating porcine single-chain antibodies (transbodies) against nonstructural protein 1β (NSP1β) of porcine reproductive and respiratory syndrome virus inhibit virus replication. Archives of Virology, 2023, 1685133.

[142] TiwariPM, ErogluE, BawageSS, VigK, MillerME, PillaiS, DennisVA, SinghSR. Enhanced intracellular translocation and biodistribution of gold nanoparticles functionalized with a cell-penetrating peptide (VG-21) from vesicular stomatitis virus. Biomaterials, 2014, 35359484-9494.

[143] TodaroB, OttalaganaE, LuinS, SantiM. Targeting Peptides: The New Generation of Targeted Drug Delivery Systems. Pharmaceutics, 2023, 1561648.

[144] TopuzoĞullari, M., Acar, T., Pelİt Arayici, P., UÇar, B., UĞurel, E., Abamor, E. Ş., ArasoĞlu, T., Turgut-Balik, D., & Derman, S. (2020). An insight into the epitope-based peptide vaccine design strategy and studies against COVID-19. Turkish Journal of Biology = Turk Biyoloji Dergisi, 44(3), 215–227. https://doi.org/10.3906/biy-2006-1

[145] TrabuloS, CardosoAL, ManoM, De LimaMCP. Cell-Penetrating Peptides-Mechanisms of Cellular Uptake and Generation of Delivery Systems. Pharmaceuticals (Basel, Switzerland), 2010, 34961-993.

[146] Uddin, M. B., Tanni, F., Hoque, S. F., Sajib, E. H., Faysal, M. A., Rahman, M., Galib, A., Emon, A. A., Hossain, M. M., Hasan, M., et al. (2022). A candidate multiepitope vaccine against Lumpy Skin Disease. Transboundary and Emerging Diseases, 69. https://doi.org/10.1111/tbed.14718

[147] Valdez-MiramontesCE, De Haro-AcostaJ, Aréchiga-FloresCF, Verdiguel-FernándezL, Rivas-SantiagoB. Antimicrobial peptides in domestic animals and their applications in veterinary medicine. Peptides, 2021, 142. 170576

[148] VasilchenkoAS, DymovaVV, KartashovaOL, SychevaMV. Morphofunctional reaction of bacteria treated with antimicrobial peptides derived from farm animal platelets. Probiotics and Antimicrobial Proteins, 2015, 7160-65.

[149] WangC, WenW-Y, SuC-X, GeF-F, DangZ-G, DuanX-G, CaoR-B, ZhouB, ChenP-Y. Bursin as an adjuvant is a potent enhancer of immune response in mice immunized with the JEV subunit vaccine. Veterinary Immunology and Immunopathology, 2008, 1223–4265-274.

[150] WangF, FengX, ZhengQ, HouH, CaoR, ZhouB, LiuQ, LiuX, PangR, ZhaoJ, et al. . Multiple linear epitopes (B cell, CTL and Th) of JEV expressed in recombinant MVA as multiple epitope vaccine induces a protective immune response. Virology Journal, 2012, 9: 204.

[151] WangY, LiJ, DaiX, WangZ, NiX, ZengD, ZengY, ZhangD, PanK. Effects of Antimicrobial Peptides Gal-13 on the Growth Performance, Intestinal Microbiota, Digestive Enzyme Activities, Intestinal Morphology, Antioxidative Activities, and Immunity of Broilers. Probiotics and Antimicrobial Proteins, 2023, 153694-705.

[152] WangD, YuP, SheR, WangK. Protective effects of rabbit sacculus-derived antimicrobial peptides on SPF chicken against infection with very virulent infectious bursal disease virus. Poultry Science, 2024, 1037. 103797

[153] WeiJ, HuangY, ZhongD, KangL, IshagH, MaoX, CaoR, ZhouB, ChenP. Design and evaluation of a multiepitope peptide against Japanese encephalitis virus infection in BALB/c mice. Biochemical and Biophysical Research Communications, 2010, 3964787-792.

[154] WeiX, ZhangL, ZhangR, WuR, PetitteJN, HouY, SiD, AhmadB, GuoH, ZhangM, et al. . Targeting the TLR2 Receptor With a Novel Thymopentin-Derived Peptide Modulates Immune Responses. Frontiers in Immunology, 2021, 12. 620494

[155] WenY, ChenR, YangJ, YuE, LiuW, LiaoY, WenY, WuR, ZhaoQ, DuS, et al. . Identification of potential SLA-I-specific T-cell epitopes within the structural proteins of porcine deltacoronavirus. International Journal of Biological Macromolecules, 2023, 251. 126327

[156] XiaT, WangN, TangY, GaoY, GaoC, HaoJ, JiangY, WangX, ShanZ, LiJ, et al. . Delivery of antigen to porcine dendritic cells by fusing antigen with porcine dendritic cells targeting peptide. Frontiers in Immunology, 2022, 13. 926279

[157] XiaT, LuX, KongD, GuoT, GaoY, XinL, JiangY, WangX, ShanZ, LiJ, et al. . Screening optimal DC-targeting peptide to enhance the immune efficacy of recombinant Lactobacillus expressing RHDV VP60. Virulence, 2024, 1512368080.

[158] XiongD, SongL, ZhaiX, GengS, PanZ, JiaoX. A porcine reproductive and respiratory syndrome virus (PRRSV) vaccine candidate based on the fusion protein of PRRSV glycoprotein 5 and the Toll-like Receptor-5 agonist Salmonella Typhimurium FljB. BMC Veterinary Research, 2015, 11: 121.

[159] XuY-G, GuanX-T, LiuZ-M, TianC-Y, CuiL-C. Immunogenicity in Swine of Orally Administered Recombinant Lactobacillus plantarum Expressing Classical Swine Fever Virus E2 Protein in Conjunction with Thymosin α-1 as an Adjuvant. Applied and Environmental Microbiology, 2015, 81113745-3752.

[160] XuY, DongM, WangQ, SunY, HangB, ZhangH, HuJ, ZhangG. Soluble Expression of Antimicrobial Peptide BSN-37 from Escherichia coli by SUMO Fusion Technology. The Protein Journal, 2023, 425563-574.

[161] YangT, WangH-N, WangX, TangJ-N, LuD, ZhangY-F, GuoZ-C, LiY-L, GaoR, KangR-M. The protective immune response against infectious bronchitis virus induced by multiepitope based peptide vaccines. Bioscience, Biotechnology, and Biochemistry, 2009, 7371500-1504.

[162] YangD, ZhangC, ZhaoL, ZhouG, WangH, YuL. Identification of a conserved linear epitope on the VP1 protein of serotype O foot-and-mouth disease virus by neutralizing monoclonal antibody 8E8. Virus Research, 2011, 1551291-299.

[163] YangL, LuX, FangW. Expression and purification of classical swine fever virus E2 protein from Sf9 cells using a modified vector. Biotechnology Letters, 2017, 39121821-1825.

[164] YangS, ZhangX, CaoY, LiS, ShaoJ, SunS, GuoH, YinS. Identification of a new cell-penetrating peptide derived from the african swine fever virus CD2v protein. Drug Delivery, 2021, 281957-962.

[165] YangH, SunM, QiuH, XuH, DengZ, GuH, WangN, DuL, ShiF, ZhouJ, et al. . Nanobody peptide conjugate: A novel CD163 based broad neutralizing strategy against porcine reproductive and respiratory syndrome virus. Journal of Nanobiotechnology, 2024, 221388.

[166] YaoY, WangJ, LiuY, QuY, WangK, ZhangY, ChangY, YangZ, WanJ, LiuJ, et al. . Variants of the adeno-associated virus serotype 9 with enhanced penetration of the blood-brain barrier in rodents and primates. Nature Biomedical Engineering, 2022, 6111257-1271.

[167] YinQ, LuoW, MallajosyulaV, BoY, GuoJ, XieJ, SunM, VermaR, LiC, ConstantzCM, et al. . A TLR7-nanoparticle adjuvant promotes a broad immune response against heterologous strains of influenza and SARS-CoV-2. Nature Materials, 2023, 223380-390.

[168] YuW, ZhanY, XueB, DongY, WangY, JiangP, WangA, SunY, YangY. Highly efficient cellular uptake of a cell-penetrating peptide (CPP) derived from the capsid protein of porcine circovirus type 2. The Journal of Biological Chemistry, 2018, 2933915221-15232.

[169] ZeiglerDF, GageE, RoqueR, CleggCH. Epitope targeting with self-assembled peptide vaccines. NPJ Vaccines, 2019, 4: 30.

[170] ZhangG, LiuW, GaoZ, YangS, ZhouG, ChangY, MaY, LiangX, ShaoJ, ChangH. Antigenicity and immunogenicity of recombinant proteins comprising African swine fever virus proteins p30 and p54 fused to a cell-penetrating peptide. International Immunopharmacology, 2021, 101Pt A. 108251

[171] ZhangQ-Y, YanZ-B, MengY-M, HongX-Y, ShaoG, MaJ-J, ChengX-R, LiuJ, KangJ, FuC-Y. Antimicrobial peptides: Mechanism of action, activity and clinical potential. Military Medical Research, 2021, 8148.

[172] ZhangY, GuoP, MaZ, LuP, KebebeD, LiuZ. Combination of cell-penetrating peptides with nanomaterials for the potential therapeutics of central nervous system disorders: A review. Journal of Nanobiotechnology, 2021, 191255.

[173] ZhangR, XuL, DongC. Antimicrobial Peptides: An Overview of their Structure, Function and Mechanism of Action. Protein and Peptide Letters, 2022, 298641-650.

[174] ZhangH, ZhangY, ZhangC, YuH, MaY, LiZ, ShiN. Recent Advances of Cell-Penetrating Peptides and Their Application as Vectors for Delivery of Peptide and Protein-Based Cargo Molecules. Pharmaceutics, 2023, 1582093.

[175] ZhangY, NaD, ZhangW, LiuX, MiaoS, TanW-S, ZhaoL. Development of stable HEK293T cell pools expressing CSFV E2 protein: A potential antigen expression platform. Vaccine, 2023, 4191573-1583.

[176] ZhaoT, CaiY, JiangY, HeX, WeiY, YuY, TianX. Vaccine adjuvants: Mechanisms and platforms. Signal Transduction and Targeted Therapy, 2023, 81283.

[177] Zhao, Y., Yu, J., Su, Y., Shu, Y., Ma, E., Wang, J., Jiang, S., Wei, C., Li, D., Huang, Z., et al. (2025). A unified deep framework for peptide–major histocompatibility complex–T-cell receptor binding prediction. Nature Machine Intelligence, 1–11. https://doi.org/10.1038/s42256-025-01002-0

[178] ZhongD, LuZ, XiaY, WuH, ZhangX, LiM, SongX, WangY, MoonA, QiuH-J, et al. . Ferritin Nanoparticle Delivery of the E2 Protein of Classical Swine Fever Virus Completely Protects Pigs from Lethal Challenge. Vaccines, 2024, 126629.

[179] Zhou, B., Liu, K., Jiang, Y., Wei, J.-C., & Chen, P.-Y. (2011). Multiple linear B-cell epitopes of classical swine fever virus glycoprotein E2 expressed in E.coli as multiple epitope vaccine induces a protective immune response. Virology Journal, 8, 378. https://doi.org/10.1186/1743-422X-8-378

[180] ZhuJ, HeX, BernardD, ShenJ, SuY, WolekA, IssacsB, MishraN, TianX, GarmendiaA, et al. . Identification of New Compounds against PRRSV Infection by Directly Targeting CD163. Journal of Virology, 2023, 975. e0005423

[181] ZhuJ, LiangZ, YaoH, WuZ. Identifying Cell-Penetrating Peptides for Effectively Delivering Antimicrobial Molecules into Streptococcus suis. Antibiotics (Basel, Switzerland), 2024, 138725.

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