Preparation and Characterization of Paclitaxel/Chitosan Nanosuspensions for Drug Delivery System and Cytotoxicity Evaluation In Vitro

Yongjia Liu; Fengren Wu; Yongle Ding; Bangshang Zhu; Yue Su; Xinyuan Zhu

doi:10.1007/s42765-019-00012-z

Advanced Fiber Materials ›› 2019, Vol. 1 ›› Issue (2) : 152 -162. DOI: 10.1007/s42765-019-00012-z

Research Article

Preparation and Characterization of Paclitaxel/Chitosan Nanosuspensions for Drug Delivery System and Cytotoxicity Evaluation In Vitro

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¹Instrumental Analysis Center, National Infrastructures for Translation Medicine (Shanghai), State Key Laboratory of Metal Matrix Composites, School of Chemistry and Chemical Engineering, Shanghai Jiao Tong University, 200240, Shanghai, China

²State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Donghua University, 201620, Shanghai, China

^d bshzhu@sjtu.edu.cn

Yongjia Liu

received her PhD from Shanghai Jiao Tong University supervised by Prof. Bangshang Zhu (2019); now, she is a teacher at the Instrumental Analysis Center of Shanghai Jiao Tong University, National Translational Medicine Platform. Currently, her work focuses on materials surface and property analysis, and her research focus on nanomedicine and biomaterials for treating cancers and tissue engineering.

Fengren Wu

is a Master candidate in Shanghai Jiao Tong University sypervised by Prof. Bangshang Zhu. His research work focuses on the synthesis and preparation of gold nanoparticles for biomedical application.

Yongle Ding

received his Bachelor’s degree from Shanghai Jiao Tong University in 2018. Now he is a Master student at School of Chemistry and Chemical Engineering, Shanghai Jiao Tong University. His research work focuses on the synthesis and self-assembly of nanomaterials.

Bangshang Zhu

Ph.D., Professor and Doctoral tutor, is engaged in research and teaching at the Instrumental Analysis Center & School of Chemistry and Chemical Engineering of Shanghai Jiao Tong University. His research work closely focuses on the synthesis of biomaterials, nanomedicine preparation and their application in biomedical engineering. He has published more than 70 SCI papers in international academic journals such as Angew. Chem. Int. Edit., Biomacromolecules, Biomaterials, Langmuir, Macromolecular, Carbon, etc. He also works as a reviewer for many international journals.

Yue Su

graduated from School of Life Sciences and Biotechnology at Hubei Engineering University in 1989. She received her master degree in Biology at Central China Normal University. After she got her Ph.D. degree in Biology at Wuhan University, she entered East China University of Science and Technology as a post-doctoral researcher. In 2013, she finished her post-doctoral research at Shanghai Jiao Tong University and joined SJTU as a faculty. Her research interests focus on cancer biology and chemistry biology.

Xinyuan Zhu

completed his BSc degree and MSc degree in Materials Science at Donghua University, and he obtained his Ph.D degree in Materials Science at Shanghai Jiao Tong University in China. Following academic appointments in Shanghai Jiao Tong University, he joined the BASF research laboratory at ISIS in Strasbourg as a post-doctoral researcher. He came back to Shanghai Jiao Tong University and became a full professor in 2005. His scientific interests focus on functional polymers.

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Abstract

In this study, we prepared paclitaxel/chitosan (PTX/CS) nanosuspensions (NSs) with different mass ratios of PTX and CS (1.5:2, 2:2, and 2.5:2), for controlled drug delivery purposes. For attachment and dispersion in water medium, a simple ultrasonic disruption technique was employed. The water-dispersed PTX/CS NSs exhibited a rod-shape morphology with an average diameter of 170–210 nm and average length of about 1–10 µm. Transmission electron microscopy, differential scanning calorimetry and X-ray diffraction indicated that the obtained PTX/CS NSs contain a nanocrystalline PTX phase. It was also inferred that presence of CS can promotes the crystalline nature of PTX up to 80%. In addition, efficiency of PTX loading reached over 85% in freeze-dried PTX/CS NSs, showing a slow rate of drug release in vitro for 8 days. The MTT and LDH assessments revealed that PTX/CS NSs significantly inhibit the growth of tumor cells (HeLa), while it is slightly toxic for the normal cells (NIH/3T3). Therefore, PTX/CS NSs is suggested as a potential nanodrug delivery system for cancer therapy.

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Yongjia Liu, Fengren Wu, Yongle Ding, Bangshang Zhu, Yue Su, Xinyuan Zhu. Preparation and Characterization of Paclitaxel/Chitosan Nanosuspensions for Drug Delivery System and Cytotoxicity Evaluation In Vitro. Advanced Fiber Materials, 2019, 1(2): 152-162 DOI:10.1007/s42765-019-00012-z