Hydrogel of biocompatible carbomers as matrix for elimination of neural tissue defects

Abstract

Short-term effects of biocompatible materials were examined on functional posttraumatlc recovery of rat sciatic nerve and mice spinal cord. Transected nerve regeneration across 5 mm gaps within silicone tubes filled with synthetic hydrogels of sodium salts of 3%polyacrylic acid (PAA] or 1,8% Carbopol® 971P NF or 8% sodium salt of carboxymethylcellulose were investigated. Functional recovery was assessed using the sciatic functional index until 30 days after nerve injury. Supportive influence of PAA was shown on motor and sensory function recovery of nerve. At the 30^th the survival ofL5 DRG neurons Increased in group with Carbopol by 13,3%, in case of PAA by 30,3% to compare with negative control group [plain tube without matrix). By day 30 after the operation the number of middle size neurons in group with PAA exceeds by 10,8% in comparison with Carbopol group. In these conditions the number of myelinated fibers exceeded by 10,3%. Addition of patented composition M4 consisting of amino acids and microelements to the PAA or Carbopol hydrogels improved the sensory reactions from the hind paw skin. On the model of complete transection of mice spinal cord at T9 it has been shown Increase of locomotor repair assessed in Basso, Beathie, Bresnahan (1995) open field rating scale under the influence of mixture of Carbopol and M4 composition. The received results testify that synthetic biocompatible materials PAA and Carbopol differently influence regeneration in the central and peripheral nervous system. Thus, investigated scaffolds as suitable conduit could be useful for Improving regeneration of the peripheral nerve or spinal cord after an injury.

Keywords

nerve regeneration / spinal cord / hydrogel

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