Participation of Ito-cells in histogenesis and regeneration of the liver

Abstract

There have been a lot of reports about differentiation possibilities of hepaticytes from hematopoitec stem cells or mesenchymal cells in the past decade.

The potential of hepatic epithelium differentiation from other sources besides entoderm has been analysed in the present study. A wide variety of monoclonal antibodies of epithelial, mesenchymal and stem cell markers and apoptosis were used to study the main stages of differentiation of epithelial and sinusoidal cells of the liver during norma/ ontogenesis and regeneration in human and rat. The results obtained lead to the conclusion that desmin-positive mesenchymal cells might be one of the sources of hepatocyte differentiation, with perisinusoidal cells being the most probable stem cells of the liver.

Keywords

Ito-cells / liver / regeneration

References

[1] Le Douarin N.M. An experimental analysis of liver development. Med. Biol. 1975;53:427-55.

[2] Houssaint E. Differentiation of the mouse hepatic primordium. I. An analysis of tissue interactions in hepatocyte differentiation. Cell Differ. 1980; 9: 269-79.

[3] Desmet V.J. Embryology of the liver and intrahepatic biliary tract, and an overview of malformations of the bile duct. In: Oxford textbook of clinical hepatology. Oxford-NewbYork-Tokyo: Oxford University Press; 1992: 497-519.

[4] Van Eyken P., Sciot R., Desmet V. Intrahepatic bile duct development in the rat. Lab. Invest. 1988; 59: 52-9.

[5] Fausto N. Hepatocyte differentiation and liver progenitor cells. Curr. Opin. Cell Biol. 1990;2:1036-42.

[6] Theise N.D., Badve S., Saxena R. et al. Derivation of hepatocytes from bone marrow cells of mice after radiation-induced myeloablation. Hepatology. 2000a; 31: 235-40.

[7] Theise N.D., Nimmakayalu M., Gardner R. et al. Liver from bone marrow in humans. Hepatology. 2000b; 32:11-6.

[8] Petersen B.E., Grossbard B., Hatch H. et al. Mouse A6-positive hepatic oval cells also express several hematopoietic stem cell markers. Hepatology. 2003; 37: 632-40.

[9] Higgins G.M., Anderson R.M. Experimental pathology of the liver: I. Restoration of the liver of the white rat following partial surgical removal. Arch. Pathol. 1931; 12:186-202.

[10] Shinozuka H., Kubo Y., Katyal S.L. et al. Roles of growth factors and of tumor necrosis factor-a on liver cell proliferation induced in rats by lead nitrate Lab. Invest. 1994; 71(1): 35-41.

[11] Киясов А.П. Современные технологии морфологических исследований. Методическое пособие для студентов, аспирантов и врачей-патологов. Казань: КГМУ, 2001.

[12] Latza U., Niedobitek G., Schwarting R. et al. Ber-EP4: new monoclonal antybody which distinguishes epithelia from mesothelia. J. Clin. Pathol. 1990; 43: 213-19.

[13] Pinkus G.S, Kurtin P.J. Epithelial membrane antigen - a diagnostic discriminant in surgical pathology: immunohistochemical profile in epithelial, mesenchymal, and hematopoietic neoplasms using paraffin sections and monoclonal antibodies. Hum. Pathol. 1985; 16(9): 929-40.

[14] Kakar S., Muir T., Murphy L.M. et al. Immunoreactivity of Hep Par 1 in hepatic and extrahepatic tumors and its correlation with albumin in situ hybridization in hepatocellular carcinoma. Am. J. Clin. Pathol. 2003; 119(3): 361-6.

[15] Ramos-Vara J.A., Miller M.A., Johnson G.C. Immunohistochemical characterization of canine hyperplastic hepatic lesions and hepatocellular and biliary neoplasms with monoclonal antibody hepatocyte paraffin 1 and a monoclonal antibody to cytokeratin 7. Vet. Pathol. 2001; 38(6): 636-43.

[16] Kiassov A.P., Van Eyken P„ van Pelt J.F. et al. Desmin expressing nonhematopoietic liver cells during rat liver development: An immunohistochemical and morphometric study. Differentiation. 1995; 59: 253-8.

[17] Киясов А.П., Гумерова A.A., Титова M.A. Овальные клетки - предполагаемые стволовые клетки печени или гепатобласты? Клеточная трансплантология и тканевая инженерия. 2006; 2(4): 55—8.

[18] Evarts R.P., Nakatsukasa Н., Marsden E.R. et al. Cellular and molecular changes in the early stages of chemical hepatocarcinogenesis in the rat. Cancer Res. 1990; 50: 3439-44.

[19] Paku S., Schnur J., Nagy P. et al. Origin and structural evolution of the early proliferating oval cells in rat liver. Am. J. Hepathol. 2001; 158:1313-23.

[20] Chagraoui J., Lepage-Noll A., Anjo A. et al. Fetal liver consists of cells in epithelial-to-mesenchimal transition. Blood. 2003; 101: 2973-82.

[21] Lim Y.S., Lee H.S. The expression of E-cadherin in human and rat hepatic stellate cells: evidence of epithelial-mesenchymal transition. Taechan. Kan. Hakhoe. Chi. 2002; 8: 90-9.

[22] Lim Y.S., Kim K.A., Jung J.O. et al. Modulation of cytokeratin expression during in vitro cultivation of human hepatic stellate cells: evidence of transdifferentiation from epithelial to mesenchymal phenotype. Histochem. Cell Biol. 2002;118:127-36.

[23] Senda T., Nomura R. The expression of cytokeratin in hepatic stellate cells of the cod. Arch. Histochem. Cytochem. 2003; 66:437-44.

[24] Киясов А.П., Гумерова A.A., Титова M.A. Мезенхимальноэпителиальная трансформация клеток Ито in vitro. Клеточные технологии в биологии и медицине. 2006; 3: 150-3.

[25] Ljubimova J.Y., Petrovic L.M., Wilson S.E et al. Expression of HGF, its receptor c-met, c-myc, and albumin in cirrhotic and neoplastic human livertissue. J. Histochem. Cytochem. 1997; 45(1): 79-87.

[26] Borowiak M., Garratt A.N., Wbstefeld T. et al. Met provides essential signals for liver regeneration. Proc. Natl. Acad. Soi. USA. 2004; 101(29): 10608-13.

[27] Huh C.G., Factor V.M., S6nchez A. et al. Hepatocyte growth factor/c-met signaling pathway is required for efficient liver regeneration and repair. Proc. Natl. Acad. Soi. USA. 2004; 101(13): 4477-82.

[28] Corcelle V., Stieger B., Gjinovci A. et al. Characterization of two distinct liver progenitor cell subpopulations of hematopoietic and hepatic origins. Exp.Cell Res. 2006; 312(15): 2826-36.

[29] Suskind D.L., Muench M.O. Searching for common stem cells of the hepatic and hematopoietic systems in the human fateal liver: CD34+cytokeratin 7/8+ cells express markers for stellate cells. J. Hepatol. 2004; 40: 261-8.

[30] Seo M.J., Suh S.Y., Bae Y.C. et al. Differentiation of human adipose stromal cells into hepatic lineage in vitro and in vivo. Biochem. Biophys. Res. Commun. 2005; 328: 258-64.

[31] Lee K.D., Kuo T.K., Whang-Peng J. et al. In vitro hepatic differentiation of human mesenchymal stem cells. Hepatology. 2004; 40:1256-9.

[32] Lange C., Bruns H., Kluth D. et al. Hepatocytic differentiation of mesenchymal stem cells in cocultures with fetal liver cells. World J. Gastroenterol. 2006; 12(15): 2394-97.

[33] Petersen B.E., Bowen W.C., Patrene K.D. et al. Bone marrow as a potential source of hepatic oval cells. Science. 1999; 284:1168-70.

[34] Lagasse E., Connors H., Al-Dhalimy M. et al. Purified hematopoietic stem cells can differentiate into hepatocytes in vivo. Nat. Med. 2000; 6:1229-34.

[35] Krause D.S., Theise N.D., Collector M.l. et al. Multiorgan, multi-lineage engraftment by a single bone marrow-derived stem cell. Cell. 2001; 105: 369-77.

[36] Baba S., Fujii H., Hirose T. et al. Commitment of bone marrow cells to hepatic stellate cells in mouse. J. Hepatol. 2004; 40: 255-60.

[37] Kordes C., Sawitza I., Msller-Marbach A. et al. CD133+ hepatic stellate cells are progenitor cells. Biochem. Biophys. Res. Commun. 2007; 352[2): 410-17.

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