«Niche - relief» conception for stem cells as a basis of biomimetic approach to boneand hemopoietic tissues engineering

Abstract

An affect of relief features and quantitative parametersof model mineral matrix on in vitro structure-functionalstatus of human lung prenatal stromal cells (HLPSC) andin vivo remodeling of mice bone/marrow system has beenstudied. According to data established, rough (Ra > 2 m)implants with calcium phosphate micro-arc coatings simulatethree-dimensional state of regenerating bone matrix. Suchsurfaces have own structure-functional sites (microregions)which were named by niche - relief and were necessaryfor maturation and differentiation of HLPSC to secretingosteoblasts. Maximum remodeling of mice bone/marrowsystem in heterotopic test in vivo was also observed underoptimal parameters of osteogenic niche in vitro (approximately43% average index of cellular alkaline phosphatase squareto artificial microregion area). A probable cell-molecularmechanism of influence experimental 3D-modeling of bonesurface on selection of quiescent or active state by endostealniche has been detected. It connects with activating cellularproduction of bone matrix components (alkaline phosphatase,osteocalcin, collagen, calcium phosphates) that dissociatedimensionally stromal and hemopoietic elements, and withparallel diminishing TNF secretion in intercellular medium.The data obtained develop our previous speculations aboutexistence and sizes of artificial endosteal niche for osteogenicdifferentiation of multipotent mesenchimal stromal cells.

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