Personalized cell therapy in ophthalmology. I. Method for obtaining and phenotype of autologous product
S E Avetisov , A M Subbot , A I Antochin , E A Kasparova , A A Kasparov , A S Pavliuk
Genes & Cells ›› 2011, Vol. 6 ›› Issue (2) : 38 -42.
Personalized cell therapy in ophthalmology. I. Method for obtaining and phenotype of autologous product
The method for obtaining activated in vitro by poly A:Uautologous leucocytes from peripheral blood for cornealdiseases treatment is developed. Method allows us to obtaina product with sufficient content of viable nucleated cells (upto 3106 cell/ml). Cellular composition of the product wasdetermined. Mononuclear fraction decreased in 1,3 times andgranulocyte portion is increased. Phenotype of lymphocytesin the cell product is the same as in peripheral blood, withselective loss of B-lymphocytes. The presence of CD14+/34lowcells, which are considered to be progenitors, is defined in theproduct. Using of cell product, obtained by designed technique,may be promising in clinic, because of it minimal traumatismfor the patient and simplicity.
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