Comparative analysis of osteogenic potential of multipotent mesenchymal stromal cells derived from oral mucosa and bone marrow

Abstract

This article is focused on comparative analysis of osteogenic potential of multipotent mesenchymal stromal cells (MMSC) derived from different sources: gingival mucosa and bone marrow. MMSC immunophenotype, proliferation potential and osteogenic differentiation capacity were investigated in this work. Ability of MMSC adhesion to p-tricalcium phosphate was also examined. Osteoinductive potential of tissue-engineered bone grafts (MMSCs + p-tricalcium phosphate) was shown in experiments carried out on NOD-SCID mice in heterotopic bone formation test. Results of this research demonstrated that both type of MMSC had similar surface markers and osteogenic differentiation capacity. However MMSC derived from gingival mucosa were characterized by higher proliferative activity and higher adhesion level to p-tricalcium phosphate. Taking into consideration that MMSC could be obtained easier from gingival mucosa, this source of stem cells is probably the most promising for tissue engineering of bone grafts for stomatology and maxillofacial surgery (at least in case of investigated scaffold).

Keywords

multipotent mesenchymal stromal cells / gingival mucosa / bone marrow / p-tricalcium phosphate / heterotopic bone formation test

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