THE EFFECT OF MILD HYPOBARICHYPOXIA IN PRECONDITIONING REGIMEON THE EXPRESSION OF PCREB AND NF-κBTRANSCRIPTION FACTORS IN RAT HIPPOCAMPUSBEFORE AND AFTER SEVEREHYPOXIA
A V Churilova , E A Rybnikova , T S Glushchenko , E I Tyul'kova , M O Samoylov , A V Churilova , Ye A Rybnikova , T S Glushchenko , Ye I Tiul'kova , M O Samoilov
Morphology ›› 2009, Vol. 136 ›› Issue (6) : 38 -42.
THE EFFECT OF MILD HYPOBARICHYPOXIA IN PRECONDITIONING REGIMEON THE EXPRESSION OF PCREB AND NF-κBTRANSCRIPTION FACTORS IN RAT HIPPOCAMPUSBEFORE AND AFTER SEVEREHYPOXIA
Preconditioning using threefold mild hypobaric hypoxia (HH)is known to increase the tolerance of vulnerable brain neurons to severe hypoxia and other damaging factors. In the present study, the changes of the expression of transcription factors NF-κB(nuclear factor kappa B) and CREB (cAMP response elementbinding protein) were studied in the hippocampus of rats preconditionedby mild hypoxia. Using immunocytochemical method, it was demonstrated that HH increased NF-κB and phosphorylatedCREB (pCREB) immunoreactivity in CA1-CA4 fields of the hippocampus and gyrus dentatus. It also contributed to the up-regulation of the expression of these transcription factors in the hippocampus of preconditioned rats 3-24 h following severeHH. These findings suggest that NF-κB and CREB are involvedin HH-activated mechanisms of brain tolerance development.
hippocampus / neurons / hypobaric hypoxia / preconditioning / transcription factors
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