NEURONAL DIVISION OR ENUCLEATION

Abstract

In this work, using the classical neurohistological Bielschowsky-Gros method, all the morphological phenomena were reproducedthat were earlier interpreted by many authors as the signs of neuron division, budding and fission. It is suggested that thesephenomena are associated with the effect of enucleation demonstratedin many cells of other tissue types exposed to differentphysical and chemical factors. The experiments were conductedin tissue culture, using the isolated neurons of the molluskLymnaea stagnalis, in which the neural cells were treated withactin microfilament inhibitor cytochalasin B. Phase-contrasttime-lapse video recording during 4-8 hours demonstrated the effects of nucleus displacement, ectopy and bulging up to almostcomplete fission of neuronal body. These effects reproduce the images obtained in static fixed preparations under normaland various experimental conditions. Sometimes, at the earlyexperimental stages, the bulging of cytoplasm was also detected.Control experiments in which the neurons were treated withthe culture medium containing cytochalasin B solvent dimethylsulfoxide, showed no changes in neurons during 8-hour period.It is suggested that the images, interpreted earlier as neuron divisionor fission, could be explained by inhibition of actin microfilaments, which sometimes may develop spontaneously in cellsexperiencing individual metabolic changes compromising the cytoskeleton stability maintenance.

Keywords

neuron / enucleation / nuclear ectopy / cell division / cytochalasin B

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