NOVEL CALRETININ-POSITIVE CELLSWITH POLYMORPHOUS SPINES IN MOUSEFOREBRAIN DURING EARLY POSTNATALONTOGENESIS
A V Revishchin , V E Okhotin , G V Pavlova , A V Revishchin , V Ye Okhotin , G V Pavlova
Morphology ›› 2009, Vol. 136 ›› Issue (6) : 20 -27.
NOVEL CALRETININ-POSITIVE CELLSWITH POLYMORPHOUS SPINES IN MOUSEFOREBRAIN DURING EARLY POSTNATALONTOGENESIS
Using an immunocytochemical method for calretinin (CR)detection, we have earlier described (Morfologiya, 2009 v. 135, № 3, p. 7-19) the population of previously unknown mono- and bipolar cells with polymorphous spines (PS) covering their cellbodies and processes, in adult mice forebrain structures adjacentto anterior horn of lateral ventricle. CR-positive spiny (CR+PS)cells were negative to GAD67 and were detected in the whitematter and in layers V and VI of frontal area of dorsomedial cortexclose to the cingulum, in in rostro-dorsal part of the caudatenucleus-putamen complex, anterior olfactory nucleus and in subependymallayer of the dorso-lateral angle of the lateral ventricle.In this work, the distribution of these cells in 7-day-old mice was studied. Comparative topographical analysis of definitive and early CR+PS cells demonstrated that in 7-day-old mice CR+PScells were absent from the areas of their localization in adult animals- anterior olfactory nucleus, cortical plate and inner portionof neostriatum. Meanwhile, some CR+PS-like cells were detectedin 7-day-old mice inside the rostral migratory route, close to neostriatum anterior boundary, along the dorsal border betweenneostriatum and corpus callosum, subependymal layer of lateralwall of the lateral ventricle, and in the cingulum area. These findingsare indicative of the possible postnatal appearance of CR+PScells. To test this hypothesis, the experiments were conducted in which bromodeoxyuridine (BrdU) was administered to the miceon their postnatal days 2-4 with the subsequent study of the brainsections of these animals sacrificed on their postnatal day 20.Double immunolabeling of these sections for CR and BrdU has detected the presence of CR+PS cells that contained postnatallyadministered BrdU. These results strongly suggest that, at least, some portion of CR+PS cells have their mitosis postnatally. It may be assumed, that CR-PS cells migrate to the sites of theirdistribution in adult animals from day 7 till day 20 of postnataldevelpment.
spiny calretinin-positive cells / migration / forebrain / postnatal development / mouse
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