Cultures of malarial plasmodium. V. V. Yakovlev (Leningrad Med. J., 1926, No. 10)
Kazan medical journal ›› 1927, Vol. 23 ›› Issue (3) : 349 -349.
The author tried to obtain these cultures according to Bass' and Johns' method, namely, after the seizure he took 10 cc of blood from v. mediana with a syringe, quickly transferred it to a graduated test tube with 0.1 50% dextrose at the bottom, and defibrinated by stirring with a glass rod. After defibrination, the stick and clot were removed, the test tube was sealed with a new sterile cotton plug, and placed in a thermostat at 40°-41° C. For further study, the author used red blood cells from the upper layer of sediment under the serum column - these red blood cells were carefully collected in a Pastenr tube and smears were made on slides.
Board E.
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