Functional activity of p-glycoprotein in blood-brain barrier during experimental par-kinson's syndrome

Abstract

Background. P-glycoprotein (Pgp, ABCB1-protein) is a membrane transporter with broad substrate specificity that is localized in hepatocytes, enterocytes, epithelial renal tubules, and also in tissue barriers, including blood-brain barrier (BBB). Increased Pgp activity in BBB is one of the reasons for the pharmacoresistance of a number of CNS diseases.

Aim. Analysis of Pgp functional activity in BBB during experimental Parkinson's syndrome.

Materials and Methods. The work was performed on 90 Wistar rats, divided into 3 series (n=30 in each). The 1 series (control) was subcutaneously injected sunflower oil once a day for 7 days, and Pgp activity in BBB was assessed on the 8^th day. The 2 and 3 series (pathology control) − were administered rotenone at a dose of 2.5 mg/kg once a day for 7 and 28 days respectively to simulate parkin-sonism. At the end of the experiment Pgp activity was estimated. To confirm Parkinson's syndrome, in addition to the clinical picture, level of dopamine in midbrain and striatum was determined using enzyme-linked immunosorbent assay. Pgp functional activity in BBB was assessed by the degree of penetration of its marker substrate fexofenadine into the brain after its intravenous administration at a dose of 10 mg/kg. The content of fexofenadine in the blood plasma and in brain tissue was estimated by the area under pharmacokinetic curve of the substance (in the blood or brain tissues) − AUC_0-t(plasma) or AUC_0-t(brain) respectively. To assess the BBB permeability the ratio AUC_0-t(brain) / AUC_0-t(plasma) was calculated.

Results. Rotenone administration led to the development of parkinsonism typical picture: muscle stiffness, hypokinesia, gait instability. There was a decrease in dopamine level in the striatum after 7 days by 69.6% (p=0.095), after 28 days − by 93.9% (p=0.008), in midbrain − by 72.7% (p=0.095) and 68.7% (p=0.032) respectively. Fexofenadine AUC_0-t(plasma) and AUC_0-t(brain) after its intravenous administration to control rats were 266.2 (246.4; 285.6) μg/ml*min and 5.9 (5.8;6.6) µg/g*min respectively, AUC_0-t(brain) /AUC_0-t(plasma) − 0.020 (0.019; 0.022). When rotenone was for 7 days administered − fexofenadine AUC_0-t(brain) increased 2.02 times (p=0.0163), AUC_0-t(brain) / AUC_0-t(plasma) − 2.4 times (p=0.0283). 28 days administration of rotenone led to augmentation of AUC_0-t(brain) of fexofenadine by 1.75 times (p=0.0283), AUC_0-t(brain) / AUC_0-t(plasma) − by 2.27 times (p=0.0163).

Conclusions. The development of Parkinson’s syndrome, caused by the administration of rotenone, inhibits Pgp functional activity in BBB, which is confirmed by the accumulation in the brain marker substrate of the transporter − fexofenadine.

Keywords

Parkinson's disease / Parkinson's syndrome / P-glycoprotein / functional activity / blood-brain barrier

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RIGHTS & PERMISSIONS

Chernykh I.V., Shchulkin A.V., Mylnikov P.Y., Gatsanoga M.V., Gradinar M.M., Yesenina A.S., Yakusheva E.N.