Construction of computer model in tissue morphometry

Abstract

Background. In a microscopic study of animal and human tissues, researchers are faced with the problem of their objective assessment. Descriptive microscopy is subjective and does not allow to make exact conclusions. A partial counting of some selective elements of tissue is often not sufficiently informative. Use of high-grade morphometry is a very laborious procedure, which is difficult to conduct, repeat and recheck. Descriptive microscopy does not allow to make a model of research for a comprehensive assessment of the results, which complicates making conclusions.

Aim. To solve the problem of objective assessment of tissue condition in histomorphological studies and accelerate their implementation with the help of computer modeling.

Materials and Methods. The whole process from making micropreparations to the end of their full analysis was divided into 4 stages: photographing the entire area of micropreparations using a video eyepiece microscope, counting histomorphological elements in the photos, construction of a computer model, analysis of the obtained data.

Results. An interactive computer model of the experiment was constructed, in which all parameters were combined into a single set, and a change of any value influenced the entire model. It was possible to visualize the obtained results, calculate new parameters, find out the relationship between them and to use additional tools, as, for example, machine training for finding non-obvious relationships between components or for speeding up further calculations.

Conclusions. The advantages of computer modeling consist in that it significantly accelerates histomorphological examinations, improves the quality of their processing, makes the procedure more transparent and provides scientists with more opportunities for in-depth analysis. An important advantage is that this technique is suitable for any histomorphological studies.

Keywords

morphometry / computer modeling / cystic breast / histomorphological studies / mammary glands

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RIGHTS & PERMISSIONS

Vasin A.S., Davidov V.V., Svirina J.A.