Application of enterosorbent on the basis of montmorillonite in acute diarrhea (experimental study)

Abstract

Aim. To study the antidiarrheal activity of enterosorbent on the basis of montmorillonite on the model of serotonin-induced diarrhea.

Materials and Methods. The study was conducted on laboratory mice of both sexes, acute diarrhea was induced by intraperitoneal injection of serotonin hydrochloride at the dose of 0.32 mg/kg, enterosorbent was introduced orally in the form of aqueous suspension in a wide range of doses 30 minutes before introduction of serotonin. Within 4 hours, the time of onset of diarrhea, the number of defecations, the fluid content in fecal masses were taken into account. Morphological examination of the small intestine was carried out.

Results. introduction of serotonin led to diarrhea in 100% of animals within 12.8±1.2 minutes, the number of defecations increased 2.5 times as compared to the intact animals and was 19.5±0.5 times in 4 hours, the fecal masses were predominantly semi-liquid and liquid. Use of enterosorbent coded Crim_04 at the maximum dose led to a delay in diarrhea up to 73.6±4.1 minutes, the number of defecations decreased to 9.4±0.1 with a significant solidification of feces. The antidiarrheal effect of enterosorbent was confirmed morphologically.

Conclusions. Enterosorbent on the basis of montmorillonite with laboratory code Crim_04 possesses a dose-dependent antidiarrheal effect in modeling of serotonin-induced diarrhea.

Keywords

enterosorbent / montmorillonite / diarrhea / antidiarrheal activity

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RIGHTS & PERMISSIONS

Tishin A.N., Pokrovskii M.V., Tishina O.М., Sernov L.N., Stepchenko A.A.