Ultrastructural Features of Rabbits’ Cornea in Phacoemulsification Using New Method of Adaptive Control of Infusion

Abstract

INTRODUCTION: Employees of the Department of Ophthalmology of Bashkir State Medical University, jointly with engineers of the Department of Microsurgical Equipment of Optimedservice, have developed a new method of adaptive control of infusion during phacoemulsification (PE) permitting to control the infusion flow depending on the changes in the rate of aspiration and infusion flows characteristic of a certain hydrodynamic situation, and also on the vacuum level in the aspiration line. An important aspect of safety of the new method is histological and electron microscopic examination of rabbits’ cornea in different periods after experimental PE.

AIM: To evaluate histological and electron microscopic features of the rabbits’ cornea after experimental PE using a new method of adaptive control of infusion.

MATERIALS AND METHODS: The experiment was conducted on 15 eyes of Chinchilla rabbits using a new method of adaptive control of infusion on the basis of Optimed Profi surgical system (Optimedservice, Russia). Histological and electron microscopic examinations of the cornea were performed on the 1^st, 7^th and 30^th day after surgery.

RESULTS: On the 1^st day after surgery, according to the study data, signs of moderate edema of the corneal stroma with mild separation of collagen fiber bundles were observed. In the cell cytoplasm, signs of dystrophic processes in the epithelial cells were noticeable in the form of vacuoles of different sizes. On the 7^th day after surgery, signs of alteration in the rabbits’ cornea reduced. In 30^th days after surgery, based on the data of histological and electron microscopic examinations, the cornea fully corresponded to norm.

CONCLUSION: The identified histological and electron microscopic alterations considerably diminish on the 7^th day after surgery, and on the 30^th day the ultrastructure of the cornea corresponds to the norm.

Keywords

phacoemulsification / post-occlusion surge / adaptive control of infusion / rabbits / ultrastructure of cornea

References

[1] Hashemi H, Pakzad R, Yekta A, et al. Global and regional prevalence of age-related cataract: a comprehensive systematic review and meta-analysis. Eye (Lond). 2020;34(8):1357–70. doi: 10.1038/s41433- 020-0806-3

[2] Hashemi H., Pakzad R., Yekta A., et al. Global and regional prevalence of age-related cataract: a comprehensive systematic review and meta-analysis // Eye (Lond). 2020. Vol. 34, No. 8. P. 1357–1370. doi: 10.1038/s41433-020-0806-3

[3] Boulter T, Bernhisel A, Mamalis C, et al. Phacoemulsification in review: Optimization of cataract removal in an invitro setting. Surv Ophthalmol. 2019;64(6):868–75. doi: 10.1016/j.survophthal.2019.06.007

[4] Boulter T., Bernhisel A., Mamalis C., et al. Phacoemulsification in review: Optimization of cataract removal in an invitro setting // Surv. Ophthalmol. 2019. Vol. 64, No. 6. P. 868–875. doi: 10.1016/j.survophthal.2019.06.007

[5] Benjamin L. Fluidics and rheology in phaco surgery: what matters and what is the hype? Eye (Lond). 2018;32(2):204–9. doi: 10.1038/eye.2017.299

[6] Benjamin L. Fluidics and rheology in phaco surgery: what matters and what is the hype? // Eye (Lond). 2018. Vol. 32, No. 2. P. 204–209. doi: 10.1038/eye.2017.299

[7] Kunishige T, Takahashi H. Effects of Combinations of Ophthalmic Viscosurgical Devices and Suction Flow Rates on the Corneal Endothelial Cell Damage Incurred during Phacoemulsification. J Ophthalmol. 2020;2020:2159363. doi: 10.1155/2020/2159363

[8] Kunishige T., Takahashi H. Effects of Combinations of Ophthalmic Viscosurgical Devices and Suction Flow Rates on the Corneal Endothelial Cell Damage Incurred during Phacoemulsification // J. Ophthalmol. 2020. Vol. 2020. P. 2159363. doi: 10.1155/2020/2159363

[9] Suzuki H, Igarashi T, Takahashi H. Effect of a New Phacoemul- sification and Aspiration Handpiece on Anterior Chamber Stability. J Cataract Refract Surg. 2023;49(1):91–6. doi: 10.1097/j.jcrs.0000000000001071

[10] Suzuki H., Igarashi T., Takahashi H. Effect of a New Phacoemul-sification and Aspiration Handpiece on Anterior Chamber Stability // J. Cataract Refract. Surg. 2023. Vol. 49, No. 1. P. 91–96. doi: 10.1097/j.jcrs.0000000000001071

[11] Sharif–Kashani P, Fanney D, Injev V. Comparison of occlusion break responses and vacuum rise times of phacoemulsification systems. BMC Ophthalmol. 2014;14:96. doi: 10.1186/1471-2415-14-96

[12] Sharif–Kashani P., Fanney D., Injev V. Comparison of occlusion break responses and vacuum rise times of phacoemulsification systems // BMC Ophthalmol. 2014. Vol. 14. P. 96. doi: 10.1186/1471-2415-14-96

[13] Vasavada V, Vasavada AR, Vasavada VA, et al. Real-time dynamic changes in intraocular pressure after occlusion break: comparing 2 phacoemulsification systems. J Cataract Refract Surg. 2021;47(9):1205–9. doi: 10.1097/j.jcrs.0000000000000666

[14] Vasavada V., Vasavada A.R., Vasavada V.A., et al. Real-time dynamic changes in intraocular pressure after occlusion break:comparing 2 phacoemulsification systems // J. Cataract Refract. Surg. 2021. Vol. 47, No. 9. P. 1205–1209. doi: 10.1097/j.jcrs.0000000000000666

[15] Miller KM, Dyk DW, Yalamanchili S. Experimental study of occlusion break surge volume in 3 different phacoemulsification systems. J Cataract Refract Surg. 2021;47(11):1466–72. doi: 10.1097/j.jcrs. 0000000000000651

[16] Miller K.M., Dyk D.W., Yalamanchili S. Experimental study of occlusion break surge volume in 3 different phacoemulsification systems // J. Cataract Refract. Surg. 2021. Vol. 47, No. 11. P. 1466–1472. doi: 10.1097/j.jcrs.0000000000000651

[17] Thorne A, Dyk DW, Fanney D, et al. Phacoemulsifier occlusion break surge volume reduction. J Cataract Refract Surg. 2018;44(12):1491–6. doi: 10.1016/j.jcrs.2018.01.032

[18] Thorne A., Dyk D. W., Fanney D., et al. Phacoemulsifier occlusion break surge volume reduction // J. Cataract Refract. Surg. 2018. Vol. 44, No. 12. P. 1491–1496. doi: 10.1016/j.jcrs.2018.01.032

[19] Aznabaev BM, Dibaev TI, Muhamadeev TR,et al. A method for adaptive infusion management during phacoemulsification. Patent RUS No. 2788289. 17.01.2023. (In Russ).

[20] Азнабаев Б.М., Дибаев Т.И., Мухамадеев Т.Р., и др. Способ адаптивного управления инфузией во время факоэмульсификации. Патент РФ № 2788289. 17.01.2023.

[21] European convention for the protection of vertebrate animals used for experimental and other scientific purposes. Explanatory Report — [1986] COETSER 1 (18 March 1986) [Internet]. Available at: http://www.worldlii.org/int/other/treaties/COETSER/1986/1.html. Accessed: 2023 July 14.

[22] European convention for the protection of vertebrate animals used for experimental and other scientific purposes. Explanatory Report — [1986] COETSER 1 (18 March 1986) [Интернет]. Доступно по: http://www.worldlii.org/int/other/treaties/COETSER/1986/1.html. Ссылка активна на 14.07.2023.

[23] Sarkisov DS, Perov YuL. Mikroskopicheskaya tekhnika. Moscow: Meditsina; 1996. (In Russ).

[24] Саркисов Д.С., Перов Ю.Л. Микроскопическая техника. М.: Медицина; 1996.

[25] Mencucci R, Ambrosini S, Ponchietti C, et al. Ultrasound thermal damage to rabbit corneas after simulated phacoemulsification. J Cataract Refract Surg. 2005;31(11):2180–6. doi: 10.1016/j.jcrs.2005.04.043

[26] Mencucci R., Ambrosini S., Ponchietti C., et al. Ultrasound thermal damage to rabbit corneas after simulated phacoemulsification // J. Cataract Refract. Surg. 2005. Vol. 31, No. 11. P. 2180–2186. doi: 10.1016/j.jcrs.2005.04.043

[27] Ungricht EL, Culp C, Qu P, et al. Effect of phacoemulsification fluid flow on the corneal endothelium: experimental study in rabbit eyes. J Cataract Refract Surg. 2022;48(4):481–6. doi: 10.1097/j.jcrs.0000000000000768

[28] Ungricht E.L., Culp C., Qu P., et al. Effect of phacoemulsification fluid flow on the corneal endothelium: experimental study in rabbit eyes // J. Cataract Refract. Surg. 2022. Vol. 48, No. 4. P. 481–486. doi: 10.1097/j.jcrs.0000000000000768

[29] Idrisova GM, Aznabaev BM, Musina LA, et al. Morphological changes of the rabbit’s cornea during experimental ultrasonic aspiration of lens cortex. Modern Problems of Science and Education. 2022;(3):113. Available at: https://science-education.ru/article/view?id=31767. Accessed: 2023 July 14. (In Russ). doi: 10.17513/spno.31767

[30] Идрисова Г.М., Азнабаев Б.М., Мусина Л.А., и др. Морфо- логические изменения роговицы при экспериментальной ультразвуковой аспирации кортикальных масс хрусталика кро- ликов // Современные проблемы науки и образования. 2022. № 3. С. 113. Доступно по: https://science-education.ru/article/view?id=31767. Ссылка активна на 14.07.2023. doi: 10.17513/spno.31767 16. Бикчураев Д.Р. Микроколлапсы передней камеры глаза при ультразвуковой факоэмульсификации. Дис. … канд. мед. наук. М.; 2015. Доступно по: https://search.rsl.ru/ru/record/01005562115?ysclid=lj6z3eflwt200466377. Ссылка активна на 14.07.2023.

[31] Bikchurayev DR. Mikrokollapsy peredney kamery glaza pri ul’trazvukovoy fakoemul’sifikatsii [dissertation]. Moscow; 2015. Available at: https://search.rsl.ru/ru/record/01005562115?ysclid=lj6z3eflwt200466377. Accessed: 2023 July 14. (In Russ).

[32] Gwon A. Chapter 13. The Rabbit in Cataract/IOL Surgery. In: Tsonis P.A., editor. Animal Models in Eye Research. Dayton, USA: Elsevier; 2008. P. 184–204. doi: 10.1016/B978-0-12-374169-1.00013-8

[33] Gwon A. Chapter 13. The Rabbit in Cataract/IOL Surgery. In: Tsonis PA, editor. Animal Models in Eye Research. Dayton, USA: Elsevier; 2008. P. 184–204. doi: 10.1016/B978-0-12-374169-1.00013-8

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