Changes in oral microbiota at different stages of active orthodontic treatment

Abstract

INTRODUCTION: There are no sufficient numbers of studies in the modern literature devoted to investigation of the relationship between the use of fixed orthodontic appliances and changes in the qualitative and quantitative bacterial composition at certain periods of orthodontic treatment.

AIM: To identify changes in the qualitative and quantitative composition of the microbiocenosis in the area of installed fixed appliances at different periods of orthodontic treatment.

MATERIALS AND METHODS: The study included 40 participants aged 18–44 years. All patients had a history of occlusion pathologies, dento-alveolar anomalies, and needed orthodontic treatment with use of fixed metal bracket systems. Before installing a fixed ligature vestibular metal bracket system (OC Orthodontics, USA), all patients underwent sanitation by a dental therapist, professional oral hygiene (removal of hard and soft dental deposits, tooth polishing), and, if necessary, extraction of wisdom teeth/wisdom tooth germs. The microbiocenosis was assessed in the area of intended placement of braces before orthodontic treatment, in the area of installation of braces in the period of adaptation to the fixed appliance (at 1 month after fixing the appliance) and in the period of active treatment (at 6 months after fixing the appliance).

RESULTS: On the initial examination, the frequency of detection of stabilizing microflora was 81.25%, periodontopathogenic microflora — 18.75%. In the period of adaptation, the frequency of detection of stabilizing microflora was 56.25%, the frequency of detection of periodontopathogens increased to 43.75% (p = 0.052). In the phase of active orthodontic treatment, a decrease in the frequency of detection of periodontopathogenic bacteria to 40.96% was observed, and the frequency of detection of stabilizing microflora was 59.04% (p = 0.007). The microbial number before the installation of fixed orthodontic appliances was 10⁵ ± 10² CFU/cm², in the adaptation phase — 10⁷ ± 10² CFU/cm², in the period of active treatment — 10⁵ ± 10² CFU/cm² (p = 0.008).

CONCLUSION: In the course of clinical and laboratory study, species and quantitative composition of the oral microbiocenosis was found to have characteristic features at different stages of the orthodontic treatment: before installation of fixed orthodontic appliances, the so-called ‘stabilizing’ microorganisms prevail over representatives of the group of periodontopathogens, in the period of adaptation, the proportion of periodontopathogens increases significantly with a decrease in the proportion of stabilizing microflora, in the phase of active orthodontic treatment their ratio normalizes.

Keywords

microbiocenosis / microbiota of oral cavity / fixed orthodontic appliances / periodontopathogens / adaptation to fixed orthodontic appliances / orthodontic treatment

References

[1] Simakova AA, Gorbatova LN, Gorbatova MA, et al. Systematic review of the prevalence of dentoalveolar anomalies in adults in Russia and other former soviet republics. Ortodontiya. 2022;(4):6–11. (In Russ).

[2] Симакова А.А., Горбатова Л.Н., Горбатова М.А., и др. Распространенность зубочелюстных аномалий у взрослого населения России и стран бывшего СССР: систематический обзор // Ортодонтия. 2022. № 4 (100). С. 6–11.

[3] Mitin NE, Perminov ES, Kalinovskiy SI, et al. Study of quality of life of dental patients using immediate protheses in the post-extraction period before implantation. Avicenna Bulletin. 2019;21(4):625–31. (In Russ). doi: 10.25005/2074-0581-2019-21-4-625-631

[4] Митин Н.Е., Перминов Е.С., Калиновский С.И., и др. Исследование качества жизни стоматологических больных, использующих иммедиат-протезы в период после экстракции зуба до проведения имплантации // Вестник Авиценны. 2019. Т. 21, № 4. С. 625–631. doi: 10.25005/2074-0581-2019-21-4-625-631

[5] Dudnik OV, Mamedov AA, Admakin OI, et al. Aspects of orthodontic protocol in cleft lip and palate patients. Pediatric Dentistry and Dental Prophylaxis. 2020;20(2):137–42. (In Russ). doi: 10.33925/1683-3031-2020-20-2-137-142

[6] Дудник О.В., Мамедов Ад.А., Адмакин О.И., и др. Особенности ортодонтического лечения пациентов с расщелиной губы и неба // Стоматология детского возраста и профилактика. 2020. Т. 20, № 2. С. 137–142. doi: 10.33925/1683-3031-2020-20-2-137-142

[7] Belousova AV, Emelina GV, Smolyaninov SI, et al. Impact of non-removable orthopedic devices effects on periodontal health. Modern Science: Actual Problems of Theory and Practice. Series of «Natural and Technical Sciences». 2020;(12):167–9. (In Russ). doi: 10.37882/2223–2966.2020.12.04

[8] Белоусова А.В., Емелина Г.В., Смольянинов С.И., и др. Влияние несъемных ортопедических конструкций на здоровье пародонта // Современная наука: актуальные проблемы теории и практики. Серия: Естественные и технические науки. 2020. № 12. С. 167–169. doi: 10.37882/2223–2966.2020.12.04

[9] Gus'kov AV, Abdurakhmanova MA, Nikiforov AA, et al. Diagnostic Significance of Proinflammatory Cytokines in Planning Dental Implantation in Patients with General Somatic Pathologies. I. P. Pavlov Russian Medical Biological Herald. 2023;31(3):501–10. (In Russ). doi: 10.17816/PAVLOVJ108371

[10] Гуськов А.В., Абдурахманова М.А., Никифоров А.А., и др. Диагностическая значимость провоспалительных цитокинов при планировании дентальной имплантации у пациентов с общесоматическими патологиями // Российский медико-биологический вестник имени академика И.П. Павлова. 2023. Т. 31, № 3. C. 501–510. doi: 10.17816/PAVLOVJ108371

[11] Yablokova NA, Sevbitov AV. Role of radiation factor in pediatric dental morbidity. Rossiyskiy Vestnik Perinatologii i Pediatrii. 2012;(6):82–7. (In Russ).

[12] Яблокова Н.А., Севбитов А.В. Роль радиационного фактора в формировании стоматологической заболеваемости детского населения // Российский вестник перинатологии и педиатрии. 2012. № 6. С. 83–87.

[13] Kiryushin VA, Bobotina NA, Demchenko MA, et al. Influence of Atmospheric Air Pollution on Frequency of Congenital Anomalies (on an example of a region). I. P. Pavlov Russian Medical Biological Herald. 2023;31(1):29–36. (In Russ). doi: 10.17816/PAVLOVJ109333

[14] Кирюшин В.А., Боботина Н.А., Демченко М.А., и др. Влияние загрязнений атмосферного воздуха на частоту врожденных пороков развития (на примере региона) // Российский медико-биологический вестник имени академика И. П. Павлова. 2023. Т. 31, № 1. С. 29–36. doi: 10.17816/PAVLOVJ109333

[15] Rogozhnikov GI, Kazymov KP, Chetvertnykh VA, et al. Morpho-structural changes of hard tissues of teeth with varying degrees of abrasion. Rossiyskiy Stomatologicheskiy Zhurnal. 2014;(1):15–8. (In Russ).

[16] Рогожников Г.И., Казымов К.П., Четвертных В.А., и др. Морфоструктурные изменения твердых тканей зубов при различной степени стираемости // Российский стоматологический журнал. 2014. № 1. С. 15–18.

[17] Kosyuga SYu, Botova DI, Buhnin AV. Prediction of the development of caries in patients of the active period ortodonic treatment. Meditsinskiy Al’manakh. 2022;(4):68–73. (In Russ).

[18] Косюга С.Ю., Ботова Д.И., Бухнин А.В. Прогнозирование развития кариеса зубов у пациентов на активном этапе ортодонтического лечения // Медицинский альманах. 2022. № 4 (73). С. 68–73.

[19] Akhmedova MD, Grigor’yeva OV, Sokolova OA, et al. Mikrobiota polosti rta pri ortodonticheskom lechenii breket-sistemami. In: The First Bukhara International conference of medical students and youth; Bukhara, 23–25 May 2019. Bukhara; 2019;1:347–9. (In Russ).

[20] Ахмедова М.Д., Григорьева О.В., Соколова О.А., и др. Микробиота полости рта при ортодонтическом лечении брекет-системами. В сб.: Первая бухарская международная конференция студентов-медиков и молодежи; Бухара, 23–25 Мая 2019 г. Бухара; 2019. Т. 1. С. 347–349.

[21] Belyayev AF, Karpenko NA. Bolevoy sindrom pri adaptatsii k breket-sistemam. Russian Journal of Pain. 2013;(1):75–6. (In Russ).

[22] Беляев А.Ф., Карпенко Н.А. Болевой синдром при адаптации к брекет-системам // Российский журнал боли. 2013. № 1 (38). С. 75–76.

[23] Miyoshi T, Oge S, Nakata S, et al. Gemella haemolysans inhibits the growth of the periodontal pathogen Porphyromonas gingivalis. Sci Rep. 2021;11(1):11742. doi: 10.1038/s41598-021-91267-3

[24] Miyoshi T., Oge S., Nakata S., et al. Gemella haemolysans inhibits the growth of the periodontal pathogen Porphyromonas gingivalis // Sci. Rep. 2021. Vol. 11, No. 1. P. 11742. doi: 10.1038/s41598-021-91267-3

[25] Tsarev VN, Nikolaeva EN, Ippolitov EV. Periodontophatogenic bacteria of the main factors of emergence and development of periodontitis. Journal of Microbiology, Epidemiology and Immuno-biology. 2017;94(5):101–12. (In Russ). doi: 10.36233/0372-9311-2017-5-101-112

[26] Царев В.Н., Николаева Е.Н., Ипполитов Е.В. Пародонтопатогенные бактерии — основной фактор возникновения и развития пародонтита // Журнал микробиологии, эпидемиологии и иммунобиологии. 2017. Т. 94, № 5. С. 101–112. doi: 10.36233/0372-9311-2017-5-101-112

[27] Gordina J. Influence of fixed orthodontic technics оn a condition of microflora of an oral cavity on various phases of orthodontic treatment. In: IV Mezhdunarodnaya studencheskaya nauchnaya konferentsiya «Studencheskiy Nauchnyy Forum — 2012»; Moscow, 15 February – 31 March 2012. Moscow; 2012. P. 1–5. Available at: https://scienceforum.ru/2012/article/2012000852?ysclid=m3n2kk18rh588503429. Accessed: 2023 March 27. (In Russ).

[28] Гордина Ю.А. Влияние несъемной ортодонтической техники на состояние микрофлоры полости рта на различных фазах ортодонтического лечения. В сб.: IV Международная студенческая научная конференция «Студенческий научный форум — 2012»; Москва, 15 февраля по 31 марта 2012 г. М.; 2012. С. 1–5. Доступно по: https://scienceforum.ru/2012/article/2012000852?ysclid=m3n2kk18rh588503429. Ссылка активна на 27.03.2023.

[29] Arab S, Nouhzadeh Malekshah S, Abouei Mehrizi E, et al. Effect of Fixed Orthodontic Treatment on Salivary Flow, pH and Microbial Count. J Dent (Tehran). 2016;13(1):18–22.

[30] Arab S., Nouhzadeh Malekshah S., Abouei Mehrizi E., et al. Effect of Fixed Orthodontic Treatment on Salivary Flow, pH and Microbial Count // J. Dent. (Tehran). 2016. Vol. 13, No. 1. P. 18–22.

[31] Shukla C, Maurya R, Singh V, et al. Evaluation of role of fixed orthodontics in changing oral ecological flora of opportunistic microbes in children and adolescent. J Indian Soc Pedod Prev Dent. 2017;35(1):34–40. doi: 10.4103/0970-4388.199226

[32] Shukla C., Maurya R., Singh V., et al. Evaluation of role of fixed orthodontics in changing oral ecological flora of opportunistic microbes in children and adolescent // J. Indian Soc. Pedod. Prev. Dent. 2017. Vol. 35, No. 1. P. 34–40. doi: 10.4103/0970-4388.199226

[33] Shukla C, Maurya RK, Singh V, et al. Evaluation of changes in Streptococcus mutans colonies in microflora of the Indian population with fixed orthodontics appliances. Dent Res J (Isfahan). 2016; 13(4):309–14. doi: 10.4103/1735-3327.187876

[34] Shukla C., Maurya R.K., Singh V., et al. Evaluation of changes in Streptococcus mutans colonies in microflora of the Indian population with fixed orthodontics appliances // Dent. Res. J. (Isfahan). 2016. Vol. 13, No. 4. P. 309–314. doi: 10.4103/1735-3327.187876

[35] Ghijselings E, Coucke W, Verdonck A, et al. Long-term changes in microbiology and clinical periodontal variables after completion of fixed orthodontic appliances. Orthod Craniofac Res. 2014;17(1):49–59. doi: 10.1111/ocr.12031

[36] Ghijselings E., Coucke W., Verdonck A., et al. Long-term changes in microbiology and clinical periodontal variables after completion of fixed orthodontic appliances // Orthod. Craniofac. Res. 2014. Vol. 17, No. 1. P. 49–59. doi: 10.1111/ocr.12031

[37] Tsarev VN, Makeyeva IM, Yagodina EA, et al. Herpesand papillomaviruses as components of microbiome in diseases of oral mucosa and periodontium. Dental Forum. 2017;(3):46-49. (In Russ).

[38] Царёв В.Н., Макеева И.М., Ягодина Е.А., и др. Герпес и папилломавирусы как компоненты микробиома при патологии слизистой оболочки полости рта и пародонта // Dental Forum. 2017. № 3. С. 46-49.

[39] Kamma JJ, Slots J. Herpesviral-bacterial interactions in aggressive periodontitis. J Clin Periodontol. 2003;30(5):420–6. doi: 10.1034/j.1600-051x.2003.20002.x

[40] Kamma J.J., Slots J. Herpesviral-bacterial interactions in aggressive periodontitis // J. Clin. Periodontol. 2003. Vol. 30, No. 5. P. 420–426. doi: 10.1034/j.1600-051x.2003.20002.x

[41] Samusenkov VO, Tsarev VN, Ippolitov EV, et al. Results of clinical and laboratory research in the complex treatment of mild chronic periodontitis using photodynamic therapy. International Dental Revue. 2021;(2):38–41. (In Russ). doi: 10.35556/idr-2021-2(95)38-41

[42] Самусенков В.О., Царев В.Н., Ипполитов Е.В., и др. Результаты клинико-лабораторных исследований при комплексном лечении хронического пародонтита легкой степени с использованием фотодинамической терапии // Стоматология для всех. 2021. № 2 (95). С. 38–41. doi: 10.35556/idr-2021-2(95)38-41

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