Morphological and Molecular Characterization of Eimeria saudiensis From Arabian Oryx (Oryx leucoryx) Held in Captivity in the Sultanate of Oman

Abstract

An observational study was conducted to investigate Eimeria species prevalence, fecal shedding, morphometric, and molecular composition in captive Arabian oryx (Oryx leucoryx) bred at the Mammals Breeding Center in Oman. Oryx were sampled in the summer and winter seasons. Eimeria was detected at a prevalence of 26% in summer and 22% in winter, with higher shedding intensity observed during the summer among adult oryx. The isolated Eimeria displayed ellipsoidal oocysts and ovoidal sporocysts with average dimensions of 30.6 × 24.3 μm (ranging from 24.8 to 35.4 × 20.9–26.7) and 14.3 × 7.1 μm (ranging from 11.1 to 18.3 × 6.1–8.6), respectively. Molecularly (18S rRNA, ITS-1), E. saudiensis revealed its unique identity and closest relation (96.7%–99.3%) to E. bovis isolates of bovine origin (MK691697 and AB769578), respectively, in the absence of antelope-derived isolates. The findings serve as a precursor to incorporating Eimeria species derived from Arabian oryx into the molecular map.

Keywords

18S rRNA / Arabian oryx / Eimeria saudiensis / ITS-1 / Oman

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