Construction of an antibiotic-free orotic acid producer in Escherichia coli via modular molecular engineering combined with a plasmid stabilization system

Zhenhui Pan , Mi Tang , Keren Sun , Yixuan Zhao , Qisheng Sun , Peng Wen , Landuo Sui , Taowei Yang , Xuewei Pan , Zhiming Rao

Systems Microbiology and Biomanufacturing ›› 2026, Vol. 6 ›› Issue (2) : 50

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Systems Microbiology and Biomanufacturing ›› 2026, Vol. 6 ›› Issue (2) :50 DOI: 10.1007/s43393-026-00451-x
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Construction of an antibiotic-free orotic acid producer in Escherichia coli via modular molecular engineering combined with a plasmid stabilization system
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Orotic acid / Pyrimidine nucleotide / Metabolic engineering / Plasmid stabilization / Escherichia coli

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Zhenhui Pan, Mi Tang, Keren Sun, Yixuan Zhao, Qisheng Sun, Peng Wen, Landuo Sui, Taowei Yang, Xuewei Pan, Zhiming Rao. Construction of an antibiotic-free orotic acid producer in Escherichia coli via modular molecular engineering combined with a plasmid stabilization system. Systems Microbiology and Biomanufacturing, 2026, 6(2): 50 DOI:10.1007/s43393-026-00451-x

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Funding

Key Technologies Research and Development Program(2024YFF1106400)

National Natural Science Foundation of China(32470067)

Fundamental Research Funds for the Central Universities(JUSRP124018)

Jiangsu Provincial Frontier Technology Research and Development Program(BF2024012)

Basic Research Program of Jiangsu(BK20233003)

RIGHTS & PERMISSIONS

Jiangnan University

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