Construction of an antibiotic-free orotic acid producer in Escherichia coli via modular molecular engineering combined with a plasmid stabilization system
Zhenhui Pan , Mi Tang , Keren Sun , Yixuan Zhao , Qisheng Sun , Peng Wen , Landuo Sui , Taowei Yang , Xuewei Pan , Zhiming Rao
Systems Microbiology and Biomanufacturing ›› 2026, Vol. 6 ›› Issue (2) : 50
Orotic acid / Pyrimidine nucleotide / Metabolic engineering / Plasmid stabilization / Escherichia coli
| [1] |
|
| [2] |
|
| [3] |
|
| [4] |
|
| [5] |
|
| [6] |
|
| [7] |
|
| [8] |
|
| [9] |
|
| [10] |
|
| [11] |
Takayama K. Process for producing orotic acid by fermentation. JUSTIA Pat. 1991. https://patents.justia.com/patent/5013656 |
| [12] |
|
| [13] |
|
| [14] |
|
| [15] |
|
| [16] |
|
| [17] |
Saleski TE, Chung MT, Carruthers DN, Khasbaatar A, Kurabayashi K, Lin XN. Optimized gene expression from bacterial chromosome by high-throughput integration and screening. Sci Adv. 2021;7(7). https://doi.org/10.1126/sciadv.abe1767. |
| [18] |
|
| [19] |
|
| [20] |
|
| [21] |
|
| [22] |
|
| [23] |
|
| [24] |
|
| [25] |
|
| [26] |
|
| [27] |
|
| [28] |
|
| [29] |
|
| [30] |
|
| [31] |
|
| [32] |
|
| [33] |
|
| [34] |
|
| [35] |
François JM, Lachaux C, Morin N. Synthetic biology applied to carbon conservative and carbon dioxide recycling pathways. Front Bioeng Biotech. 2020;7–2019. https://doi.org/10.3389/fbioe.2019.00446. |
| [36] |
|
| [37] |
|
| [38] |
|
| [39] |
|
| [40] |
|
| [41] |
|
| [42] |
|
| [43] |
|
| [44] |
|
| [45] |
|
| [46] |
|
| [47] |
|
| [48] |
|
| [49] |
|
| [50] |
|
| [51] |
|
| [52] |
|
| [53] |
|
| [54] |
|
| [55] |
|
| [56] |
|
| [57] |
|
Jiangnan University
/
| 〈 |
|
〉 |