Metabolic engineering of Klebsiella pneumoniae for enhanced 1,3-propanediol production

Abstract

1,3-propanediol (1,3-PDO) is an important monomer for polyester production with broad industrial applications. Klebsiella pneumoniae is currently recognized as a highly efficient producer of 1,3-PDO. However, the higher cost of glycerol substrate compared with glucose limits the economic feasibility of this route. In the present study, the K. pneumoniae strain FMME-KP was extensively engineered for efficient 1,3-PDO production from glucose. Strategies included the introduction of a glycerol synthetic pathway from glucose, balancing carbon distribution between biomass formation and product synthesis, alleviating carbon catabolite repression, and strengthening the 1,3-PDO biosynthetic route. The optimized strain, K. pneumoniae GZ31, produced 84.7 g/L of 1,3-PDO, achieving a yield of 0.51 g/g and a productivity of 1.74 g/L/h.

Keywords

1,3-Propanediol / Metabolic engineering / Klebsiella pneumoniae / Glucose substrate

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Funding

National Natural Science Foundation of China(22378164, 32470059)

RIGHTS & PERMISSIONS

Jiangnan University