Study of tannic acid stress on bacteria and role of gal enzyme claster for gallic acid metabolism to higher extent through molecular docking with special reference to Stenotrophomonas maltophilia PKA14

Abstract

Tannic acid (TA), the secondary metabolite of plant, produced to protect themselves from predators. It forms chelat to precipitate out the dietary as well as extracellular protein molecules. Present study depicted the adaptation strategies of tannase producing Stenotrophomonas maltophilia PKA14 (Accession No.: KY921597) to avail nutrient in TA environment. A thick outer layer of bacteria composed of carbohydrate was observed during TA exposure which gradually become thinner with the production of tannase. Initially, non-reducing sugar concentration was high to protect the cell from TA stress. With time, the non-reducing sugar gradually converted to reducing sugar to supply carbon source to entrapped vegetative cell. Regulation of gallic acid, the product of tannase hydrolysis, using Gal cluster gene were also analysed through in silico molecular docking. This observation could be applicable for tannase producing microbiome study, growth optimization of tannase producing industrial culture and enhancement of industrial production of gallic acid.

Graphical abstract

Complete illustration of tannic acid stress management by tannase producing bacteria, tannic acid degradation and utilization of glucose and gallic acid.

Keywords

Bacteria / Tannic acid adaptation / Tannase / Gal enzyme cluster / Gallic acid metabolism / Molecular docking

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