Bacillus species: potential methanol bioconversion platform

Abstract

Methanol has emerged as promising single-carbon and sustainable feedstocks with no association with food production. Methanol bioconversion by methylotrophic fermentations has been practiced for decades, but their applications are still limited to natural methylotrophs due to the lack of synthetic biology tools and suitable classis. Bacillus species generally has good resistance and safety, and are potential chassis in industry. Moreover, some Bacillus species are natural methylotrophs, which means that Bacillus species employed as methanol bioconversion classis may be feasible. This review systematically analyzes methanol metabolic pathways in natural methylotrophs, focusing on three critical biochemical processes: methanol oxidation, formaldehyde assimilation, and dissimilation pathways. Additionally, the article provides a comprehensive discussion of applications existing challenges in methylotrophic Bacillus engineering, including those related to methanol oxidation, formaldehyde toxicity, cofactor balance, and fermentation equipment, while proposing potential strategies to address these technical bottlenecks in the construction of Bacillus methanol classis. This review seeks to demonstrate the potential of the Bacillus species in next-generation methanol-based applications and provide a practical reference for researchers selecting methylotrophic strains.

Keywords

Methanol metabolism / Methanol bioconversion / Bacillus species / Methylotroph / Classis

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Funding

the National Key Research & Development Program of China(2020YFA0907700)

the Basic Research Program of Jiangsu and supported by the Jiangsu Basic Research Center for Synthetic Biology(BK20233003)

Wuxi Industrial Innovation Research Institute Pilot Technology Pre-research Project(XD24024)

the National Natural Foundation of China(32172174)

the Jiangsu Funding Program for Excellent Postdoctoral Talent (2024ZB371)

RIGHTS & PERMISSIONS

Jiangnan University